Ụdị Ọrịa Iku Oku Iri na Anọ Ejikọtara
Aha ngwaahịa
HWTS-RT159B Ụdị Ọrịa Ikuku Iri na Anọ Ngwa Nchọpụta Asịd Nucleic (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa iku ume bụ ọrịa kachasị na-efe efe n'ime mmadụ, nke nwere ike ime n'ụdị nwoke na nwanyị, afọ na mpaghara ọ bụla. Ọ bụ otu n'ime ihe ndị kacha akpata ọrịa na ọnwụ n'etiti ndị mmadụ n'ụwa niile.[1]Ọrịa ndị a na-ahụkarị n'ikuku ume gụnyere coronavirus ọhụrụ, nje Influenza A, nje Influenza B, nje respiratory syncytial, Adenovirus, nje metapneumovirus mmadụ, nje rhinovirus, ụdị nje Parainfluenza I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, na Streptococcus pneumoniae, wdg.[2,3].
Ọwa
| Ọnọdụ Ọkụ | Aha Ngwọta Mmeghachi Omume | Ihe ndị na-akpata ọrịa a ga-achọpụta |
| 1 | Ngwakọta Ukwu A | SARS-CoV-2, IFV A, IFV B |
| 2 | Ngwakọta Ukwu B | Nkwado, hMPV, MP, Cpn |
| 3 | Ngwakọta Ukwu C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Ngwakọta Ukwu D | CoV, EV, SP, njikwa dị n'ime |
| 5 | Ngwakọta Ukwu A | SARS-CoV-2, IFV A, IFV B |
| 6 | Ngwakọta Ukwu B | Nkwado, hMPV, MP, Cpn |
| 7 | Ngwakọta Ukwu C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Ngwakọta Ukwu D | CoV, EV, SP, njikwa dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | Swab nke imi, swab nke imi |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Mpempe akwụkwọ/mL |
| Nkọwapụta | Nnwale nnwale mmeghachi omume gosiri na enweghị mmeghachi omume mmegharị ahụ n'etiti ngwa a na Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, ụdị Mycobacterium tuberculosis attenuated, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivaryus, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiraveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii na asịd nucleic genomic nke mmadụ. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.)Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR BioRad CFX96 n'oge nkịtị, Sistemụ PCR BioRad CFX Opus 96 n'oge nkịtị |
Usoro Ọrụ
Ihe na-eme ka a na-ewepụta ihe a tụrụ aro: Ngwakọta Macro & Micro-Test Viral DNA/RNA (HWTS-3017) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Olu ihe nlele ewepụtara bụ 200µL. A ga-eme usoro ndị na-esote dịka ntuziaka maka ojiji nke ihe na-eme ka a na-ewepụta ihe a si dị. Olu ihe na-eme ka a na-ewepụta ihe a dị na ya bụ80µL.
