Ụdị 14 nke ọrịa iku ume jikọtara ọnụ
Aha ngwaahịa
HWTS-RT159B 14 Ụdị nke Ọrịa iku ume Ngwakọta Ngwa Nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Ọrịa iku ume bụ ọrịa a na-ahụkarị na ụmụ mmadụ, nke nwere ike ime na okike, afọ na mpaghara ọ bụla. Ọ bụ otu n'ime ihe na-ebute ọrịa na-anwụ anwụ na ọnụ ọgụgụ mmadụ n'ụwa niile[1]. Ọrịa ndị na-akpata iku ume na-agụnye novel coronavirus, Influenza A virus, Influenza B virus, Respiratory syncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia, pneumoniae, pneumoniae na ndị ọzọ.[2,3].
Ọwa
| Ọnọdụ ọma | Aha azịza mmeghachi omume | Ọrịa ndị a ga-achọpụta |
| 1 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, njikwa ime |
| 5 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Oropharyngeal swab, nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200 mbipụta/ml |
| Nkọwapụta | Nsonaazụ nyocha nke cross-reactivity gosiri na enweghị mmeghachi omume n'etiti kit a na Cytomegalovirus, ụdị nje virus Herpes simplex 1, nje Varicella-zoster, nje Epstein-Barr, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella, Molisinshala, Molisinhala, Molisella, Molisophila, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella, Straxella, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella, Molisella. Ụkwara nta nke mycobacterium, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas Burkholder maltophilia, Corynebacteria. Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Klebsiella pneumoniae, Klebsiella pneumoniae. |
| Ngwa ndị ọdabara | SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.)Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ Nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 ezigbo oge PCR Sistemu |
Usoro ọrụ
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (nke ike-eji na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) site Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The amịpụtara sample olu bụµL 200. Ekwesịrị ịme usoro ndị na-esote dịka ntuziaka maka ojiji nke reagent mmịpụta a. Olu elution akwadoro bụ80µL.
