Ụdị 28 nke Nje Virus papilloma mmadụ nwere nnukwu ihe ize ndụ (16/18 typing) Nucleic acid
Aha ngwaahịa
Ụdị HWTS-CC006A-28 nke Nje Virus papilloma mmadụ nwere nnukwu ihe ize ndụ (16/18) Ngwa nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Ọrịa kansa cervical bụ otu n'ime etuto ahụ jọgburu onwe ya na-esikarị n'okirikiri ọmụmụ nwanyị.Nnyocha egosiwo na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa na-efe efe bụ otu n'ime isi ihe na-ebute ọrịa kansa cervical.Ugbu a, ọgwụgwọ ndị a ma ama dị irè ka enweghị maka ọrịa kansa cervical nke HPV kpatara, yabụ n'oge nchọpụta na mgbochi nke ọrịa cervical nke HPV kpatara bụ isi ihe na-egbochi ọrịa kansa cervical.Ọ dị oke mkpa iji guzobe ule nyocha ọrịa etiology dị mfe, akọwapụtara na ngwa ngwa maka nyocha ụlọ ọgwụ na ọgwụgwọ ọrịa kansa cervical.
Ọwa
| Ngwakọta mmeghachi omume | Ọwa | Ụdị |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Njikwa ime | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Njikwa ime |
Nka nka
| Nchekwa | Mmiri: ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Cervical swab, ikpu swab, mmamịrị |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300 oyiri/ml |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke nwere ike iji na Macro & Micro-Test). Akpaaka Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 200μL nke saline nkịtị iji kwughachi pellet ahụ na nzọụkwụ 2.1, mgbe ahụ, a ga-eme mmịpụta ahụ dịka ọ dị. na ntuziaka maka ojiji nke a mmịpụta reagent.Oke elution akwadoro bụ 80μL.
Reagenti mmịpụta akwadoro: QIAamp DNA Mini Kit (51304) ma ọ bụ kọlụm Macro & Micro-Test Viral DNA/RNA (HWTS-3020-50).Tinye 200μL nke saline nkịtị ka ịmaliteghachi pellet ahụ na nzọụkwụ 2.1, mgbe ahụ, a ga-eme mmịpụta ahụ dịka ntuziaka maka ojiji nke reagent mmịpụta a.The amịpụtara sample olu nke sample bụ niile 200μL, na akwadoro elution olu bụ 100μL.
