Ụdị 28 nke Nje Virus papilloma mmadụ nwere nnukwu ihe ize ndụ (16/18 typing) Nucleic acid

Ụdị 28 nke Nje Virus papilloma mmadụ nwere nnukwu ihe ize ndụ (16/18 pịnye) Foto Nucleic Acid gosipụtara

Nkọwa dị mkpirikpi:

Ngwa a dabara adaba maka nchọpụta qualitative in vitro nke ụdị nje papilloma mmadụ 28 (HPV) (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 45, 5, 5, 5, 5, 5, 5, 5, 45, 45, 5, 5, 5, 5, 5, 5, 5, 5, 45, 45, 45, 45, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5). 61, 66, 68, 73, 81, 82, 83) nucleic acid n'ime mmamịrị nwoke / nwanyị na sel nwanyị exfoliated cervical. Enwere ike ịdepụta HPV 16/18, ụdị ndị fọdụrụ enweghị ike ịdepụta kpamkpam, na-enye ụzọ enyemaka maka nyocha na ọgwụgwọ nke ọrịa HPV.

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Ụdị HWTS-CC006A-28 nke Nje Virus papilloma mmadụ nwere nnukwu ihe ize ndụ (16/18) Ngwa nchọpụta Nucleic Acid (Fluorescence PCR)

Epidemiology

Ọrịa kansa cervical bụ otu n'ime etuto ahụ jọgburu onwe ya na-esikarị n'okirikiri ọmụmụ nwanyị. Nnyocha egosiwo na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa na-efe efe bụ otu n'ime isi ihe na-ebute ọrịa kansa cervical. Ugbu a, ọgwụgwọ ndị a ma ama dị irè ka enweghị maka ọrịa kansa cervical nke HPV kpatara, yabụ n'oge nchọpụta na mgbochi nke ọrịa cervical nke HPV kpatara bụ isi ihe na-egbochi ọrịa kansa cervical. Ọ dị oke mkpa iji guzobe ule nyocha ọrịa etiology dị mfe, akọwapụtara na ngwa ngwa maka nyocha ụlọ ọgwụ na ọgwụgwọ ọrịa kansa cervical.

Ọwa

Ngwakọta mmeghachi omume	Ọwa	Ụdị
PCR-Mix1	FAM	18
	VIC(HEX)	16
	ROX	31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68
	CY5	Njikwa ime
PCR-Mix2	FAM	6, 11, 54, 83
	VIC(HEX)	26, 44, 61, 81
	ROX	40, 42, 43, 53, 73, 82
	CY5	Njikwa ime

Nka nka

Nchekwa	Mmiri: ≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	Cervical swab, ikpu swab, mmamịrị
Ct	≤28
CV	<5.0%
LoD	300 oyiri/ml
Ngwa ndị ọdabara	Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio^®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler^®480 ezigbo oge PCR usoro Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke ike ga-eji na Macro & Micro-West akpaaka Nucleic Acid Extractor,30H6). HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 200μL nke saline nkịtị ka ọ kwụsịtụ pellet ahụ na nzọụkwụ 2.1, mgbe ahụ, a ga-eduzi mmịpụta dị ka ntuziaka maka ojiji nke reagent mmịpụta a. Oke elution akwadoro bụ 80μL.
Reagent mmịpụta akwadoro: QIAamp DNA Mini Kit (51304) ma ọ bụ Kọlụm Macro & Micro-Test Viral DNA/RNA (HWTS-3020-50). Tinye 200μL nke saline nkịtị ka ọ kwụsịtụ pellet na nzọụkwụ 2.1, mgbe ahụ, a ga-eduzi mmịpụta ahụ dịka ntuziaka maka ojiji nke reagent mmịpụta a. The amịpụtara sample olu nke sample bụ niile 200μL, na akwadoro elution olu bụ 100μL.

Nke gara aga: Enterococcus na-eguzogide Vancomycin na Gene na-eguzogide ọgwụ

Osote: Papillomavirus mmadụ (ụdị 28) Genotyping

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