Isedị abụọ nke ihe egwu dị egwu nke papalloma (16/18 na-egosi) nucleic acid
Aha
HWT-CC006A-28 ofdị Vapilloma nje na-eme ihe nkiri (16/18 Acting) Nucleic Acid Kit (Fluorescence Pc)
Mpidermiology
Certical cancer bụ otu n'ime ihe ndị na-ewu ewu na-arụ ọrụ nke nwanyị. Nnyocha e mere egosila na ọrịa hpv na ọtụtụ ọrịa bụ otu n'ime isi ihe na-akpata ọrịa cancer. Ugbu a, ọgwụgwọ dị irè a amatabeghị na HPV, yabụ nchọpụta na mgbochi na mgbochi nke HPV bụ igbochi ọrịa kansa. Ọ bụ ihe dị mkpa iji guzobe ụdị nyocha dị mfe, nke akọwapụtara nke ọma maka nyocha ọrịa na ọgwụgwọ nke ọrịa kansa.
Chaneelu
Ngwakọta mmeghachi omume | Chaneelu | Udi |
Pcr-Mix1 | M | 18 |
Vic (hex) | 16 | |
Rox | 31, 35, 35, 39, 45, 51, 52, 56, 58, 58, 59, 59, 66, 66, 68 | |
Pur5 | Nchịkwa ime | |
Pcr-Mix2 | M | 6, 11, 54, 83 |
Vic (hex) | 26, 44, 61, 81 | |
Rox | 40, 42, 43, 53, 73, 82 | |
Pur5 | Nchịkwa ime |
Nrụta Nka na ụzụ
Storaji | Mmiri: ≤-18 ℃ |
Ebe ndu | 12 ọnwa |
Ụdị ụdị | Cervic swab, swab ma ọ bụ mmamiri |
Ct | ≤28 |
CV | <5.0% |
Loo | 300copies / ml |
Ngwá agha | Ngwa Biosystems na-etinye 7500 ezigbo oge pcr Ngwa Biosystems na-etinye 7500 ngwa ngwa usoro pcr Nke quagruo®5 ezigbo oge pcr Slan-96p ezigbo oge pcr Lightcycler®480 ezigbo oge pcr sistemụ Linegene 9600 Pụnyere Real Chemical System Ma-6000 Real-Oge lyr Beired cfx96 ezigbo oge pcr sistemụ Beired cfx opus 96 Real System |
Na-arụ ọrụ
A na-atụ aro mmịpụta ntụgharị: macro & Micro-ule (HWT-3017-50, HWT-3017-97-96) (nke enwere ike iji ya macro na nwale Ackatic ackic acid (hwts-3006c, hwts-3006B)) site na Jiangsu Macro & micro-nnwale med-tech Co. Tinye 200μL tinye 200μL tinye ya na Pellet na Nzọụkwụ 2.1, mgbe ahụ, a ga-eduzi mmịpụta ya dị ka ndị mmịpụta maka ihe ndozi a. Olu akwadoro aro bụ 80μL.
A na-atụ aro mmịpụta ntụgharị: Qiap DNA MI (51304) ma ọ bụ kọlụm / RNA-3020-50). Tinye 200μL nke saline zuru oke iji wepụta Pellet na Nzọụkwụ 2.1, mgbe ahụ, a ga-eduzi mmịpụta dị ka ntuziaka maka ihe ndozi a. Olu nlele nke ihe nlele ahụ na-enye bụ ihe niile 200μL, na olu ure a tụrụ aro bụ 100μl.