Ụdị 28 nke Nje Virus Papilloma Mmadụ Dị Oke Njọ (16/18 Types) Nucleic Acid

Ụdị 28 nke Nje Virus Papilloma Mmadụ nke Nwere Ike Ịdị n'Ihe Ize Ndụ Dị Ukwuu (Ntinye 16/18) Foto A Na-egosipụta

Nkọwa Dị Mkpirikpi:

Ngwa a dabara adaba maka nchọpụta njirimara in vitro nke ụdị nje papilloma mmadụ iri abụọ na asatọ (HPV) (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) nucleic acid n'ime mmamịrị nwoke/nwanyị na mkpụrụ ndụ nwanyị a na-ewepụ akpụkpọ ahụ. Enwere ike ịpịpụta HPV 16/18, enweghị ike ịpịpụta ụdị ndị fọdụrụ kpamkpam, na-enye ụzọ enyemaka maka nchọpụta na ọgwụgwọ nke ọrịa HPV.

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Nkọwa Ngwaahịa

Akara Ngwaahịa

Aha ngwaahịa

Ụdị HWTS-CC006A-28 nke nje virus papilloma mmadụ dị oke egwu (16/18 typing) Ngwa nchọpụta nucleic acid (Fluorescence PCR)

Ọmụmụ ihe gbasara ọrịa na-efe efe

Ọrịa kansa olu bụ otu n'ime etuto ọjọọ kachasị na-eme n'ime ụmụ nwanyị. Nnyocha egosila na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa bụ otu n'ime ihe ndị bụ isi na-akpata ọrịa kansa olu. Ugbu a, a ka nwere ọgwụgwọ dị irè a ma ama maka kansa olu nke HPV kpatara, yabụ nchọpụta mbụ na mgbochi nke ọrịa olu nke HPV kpatara bụ isi ihe na-egbochi kansa olu. Ọ dị oke mkpa ịmepụta ule nchọpụta etiology dị mfe, kpọmkwem na ngwa ngwa maka nchọpụta ahụike na ọgwụgwọ nke kansa olu.

Ọwa

Ngwakọta Mmeghachi Omume	Ọwa	Ụdị
PCR-Mix1	FAM	18
	VIC (HEX)	16
	ROX	31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68
	CY5	Njikwa Dị n'ime
PCR-Mix2	FAM	6, 11, 54, 83
	VIC (HEX)	26, 44, 61, 81
	ROX	40, 42, 43, 53, 73, 82
	CY5	Njikwa Dị n'ime

Usoro Nkà na ụzụ

Nchekwa	Mmiri mmiri: ≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ihe atụ	Swab nke dị n'ime ikpu, swab nke dị n'ime ikpu, mmamịrị
Ct	≤28
CV	<5.0%
LoD	300Mpempe akwụkwọ/mL
Ngwa Ndị Dị Mkpa	Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio^®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler^®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96

Usoro Ọrụ

Ihe na-eme ka a na-ewepụta ihe: Ngwa DNA/RNA nke Macro & Micro-Test Viral (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 200μL nke saline nkịtị iji weghachite pellet ahụ na nzọụkwụ 2.1, wee mee mwepụ ahụ dịka ntuziaka maka ojiji nke ihe na-eme ka a na-ewepụta ihe a si dị. Olu mwepụta akwadoro bụ 80μL.
Ihe na-eme ka a na-ewepụta ihe a tụrụ aro: QIAamp DNA Mini Kit (51304) ma ọ bụ Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Tinye 200μL nke saline nkịtị iji weghachite pellet ahụ na nzọụkwụ 2.1, wee mee mwepụ ahụ dịka ntuziaka maka ojiji nke ihe na-eme ka a na-ewepụta ihe a si dị. Olu ihe nlele ahụ niile bụ 200μL, olu ihe nlele ahụ akwadoro bụ 100μL.

Nke gara aga: Enterococcus na-eguzogide ọgwụ na mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ ọjọọ nke Vancomycin

Osote: Mkpụrụ ndụ ihe nketa nke mmadụ (ụdị 28)

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