Ụdị 4 nke Nje Virus Nucleic Acid

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative nke SARS-CoV-2, nje influenza A, nje influenza B na nje syncytial nje nucleic acid n'ime ihe nlele swab nke oropharyngeal mmadụ.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-RT075-Freeze-Firidị 4 Ụdị Nje Virus Nchọpụta Nucleic Acid (Fluorescence PCR)

Epidemiology

Ọrịa Corona Virus 2019, nke akpọrọ "COVID-19", na-ezo aka na oyi baa nke ọrịa SARS-CoV-2 kpatara.SARS-CoV-2 bụ coronavirus nke β genus.COVID-19 bụ ọrịa na-efe efe na-efe efe na-efe efe na-efe efe, ndị mmadụ na-enwekarịkwa ike.Ka ọ dị ugbu a, isi ihe butere ọrịa bụ ndị ọrịa SARS-CoV-2 butere, yana ndị butere ọrịa asymptomatic nwekwara ike bụrụ isi iyi butere ọrịa.Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7.Ahụ ọkụ, ụkwara akọrọ na ike ọgwụgwụ bụ isi ihe ngosi.Ndị ọrịa ole na ole nwere ihe mgbaàmà dị ka imi imi, imi imi, akpịrị akpịrị, myalgia na afọ ọsịsa, wdg.

Influenza, nke a na-akpọkarị "flu", bụ nnukwu ọrịa na-efe efe nke iku ume nke nje influenza kpatara.Ọ na-efe efe nke ukwuu.A na-ebutekarị ya site na ụkwara na imi.Ọ na-apụtakarị na mmiri na oyi.A na-ekewa nje virus influenza n'ime influenza A (IFV A), influenza B (IFV B), na influenza C (IFV C) ụdị atọ, ha niile bụ nje virus na-egbuke egbuke, na-ebute ọrịa mmadụ karịsịa maka nje influenza A na B, ọ bụ otu. - stranded, nke RNA nje.Influenza A bụ nnukwu ọrịa iku ume, gụnyere H1N1, H3N2 na ụdị ndị ọzọ, nke na-enwekarị mmụgharị na ntiwapụ n'ụwa nile."Shift" na-ezo aka na ngbanwe nke nje influenza A, na-ebute mpụta nke nje ọhụrụ "subtype".A na-ekewa nje virus influenza B ụzọ abụọ, Yamagata na Victoria.Nje virus influenza B nwere naanị ihe mgbochi antigenic, ọ na-ezere nleba anya na mkpochapụ nke sistemu ahụ ji alụso ọrịa ọgụ site na ngbanwe ya.Otú ọ dị, evolushọn ọsọ nke nje influenza B dị nwayọọ karịa nke nje influenza A mmadụ.Influenza B nwekwara ike bute ọrịa iku ume mmadụ ma bute ọrịa na-efe efe.

Nje virus syncytial iku ume (RSV) bụ nje RNA, nke sitere na ezinụlọ paramyxoviridae.A na-ebute ya site na ụmụ irighiri mmiri ikuku na nso nso a na ọ bụ isi ihe na-akpata ọrịa iku ume n'ime ụmụ ọhụrụ.Ụmụaka nwere ọrịa RSV nwere ike ịmalite bronchiolitis siri ike na oyi baa, nke metụtara ụkwara ume ọkụ na ụmụaka.Ụmụaka na-enwe mgbaàmà siri ike, gụnyere nnukwu ahụ ọkụ, rhinitis, pharyngitis na laryngitis, na mgbe ahụ bronchiolitis na oyi baa.Ole na ole ụmụaka na-arịa ọrịa nwere ike mgbagwoju anya na otitis media, pleurisy na myocarditis, wdg. elu akụkụ okuku ume na tract ọrịa bụ isi ihe mgbaàmà nke ọrịa na ndị okenye na ndị okenye ụmụaka.

Ọwa

FAM SARS-CoV-2
VIC(HEX) RSV
CY5 IFV A
ROX IFV B
NED

Njikwa ime

Nka nka

Nchekwa 2-8 Celsius C
Ndụ nchekwa Ọnwa iri na abụọ
Ụdị ụdị Oropharyngeal swab
Ct ≤38
LoD SARS-CoV-2: 150 oyiri/ml

Influenza A virus/Influenza B nje/Nje virus syncytial iku ume: 300Copy/mL

Nkọwapụta Ọ dịghị cross-reactivity na mmadụ coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza nje ụdị 1, 2, 3, rhinovirus A, B, C, chlamydia. oyi baa, mmadụ metapneumovirus, enterovirus A, B, C, D, mmadụ pulmonary virus, epstein-barr virus, measles virus, mmadụ cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae. staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium ụkwara nta, anwụrụ ọkụ aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci na amụrụ ọhụrụ cryptococcus na mmadụ genomic nucleic acid.
Ngwa ndị ọdabara Ngwa ngwa Biosystems 7500 Real-Time PCR Sistemu, Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa, QuantStudio®5 Sistemụ PCR ezigbo oge

Usoro ọrụ

Nhọrọ 1.

Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test akpaka Nucleic Acid Extractor (HWTS-3006).

Nhọrọ 2.

Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech(Beijing) Co., Ltd.


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