ALDH Mkpụrụ ndụ ihe nketa Polymorphism
Aha ngwaahịa
HWTS-GE015Ngwa Nchọpụta Mkpụrụ Ndụ Ihe Mkpụrụ Ndụ Ihe nketa ALDH (ARMS -PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Mkpụrụ ndụ ALDH2 (acetaldehyde dehydrogenase 2), dị na kromozom mmadụ 12. ALDH2 nwere ọrụ esterase, dehydrogenase na reductase n'otu oge. Ọmụmụ ihe egosila na ALDH2 bụ enzyme metabolic nke nitroglycerin, nke na-agbanwe nitroglycerin ka ọ bụrụ nitric oxide, si otú a na-eme ka arịa ọbara dị jụụ ma na-emeziwanye nsogbu ọbara. Agbanyeghị, enwere polymorphisms na mkpụrụ ndụ ALDH2, nke a na-ahụkarị na East Asia. Ụdị ALDH2*1/*1 GG nke ọhịa nwere ikike metabolic siri ike, ebe ụdị heterozygous nwere naanị 6% nke ọrụ enzyme nke ụdị ọhịa, ụdị homozygous mutant enweghị ihe fọrọ nke nta ka ọ bụrụ ọrụ enzyme efu, yana metabolism adịghị ike nke ukwuu ma enweghị ike iru mmetụta achọrọ, si otú a na-emerụ ahụ mmadụ ahụ.
Ọwa
| FAM | ALDH2 |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Ọbara mgbochi ikpụkọ ọbara EDTA |
| CV | <5.0% |
| LoD | 103Mbipụta/mL |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Ihe ndị a tụrụ aro maka mwepụta: jiri Ngwa Mwepụta DNA nke Blood Genome (DP318) nke Tiangen Biotech (Beijing) Co.,Ltd. ma ọ bụ Ngwa Mwepụta Mkpụrụ Ndụ nke Blood Genome (A1120) nke Promega wepụtara iji wepụta ọbara EDTA nke ejiri ọgwụ mgbochi coagulated mee ihe.
Ihe ndị a tụrụ aro maka mwepụta: Ngwakọta DNA/RNA nke Macro & Micro-Test General (HWTS-3019) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị ime mwepụta ahụ dịka ntuziaka si dị. Olu mwepụta akwadoro bụ100μL.
