ALDH Genetic Polymorphism
Aha ngwaahịa
HWTS-GE015Ngwa ALDH Genetic Polymorphism Nchọpụta (ARMS -PCR)
Epidemiology
ALDH2 gene (acetaldehyde dehydrogenase 2), na-achọta na chromosome mmadụ 12. ALDH2 nwere esterase, dehydrogenase na reductase ọrụ n'otu oge. Nnyocha egosiwo na ALDH2 bụ enzyme metabolic nke nitroglycerin, nke na-agbanwe nitroglycerin n'ime nitric oxide, si otú ahụ na-eme ka arịa ọbara dị jụụ ma na-eme ka nsogbu na-erugharị ọbara dịkwuo mma. Agbanyeghị, enwere polymorphisms na mkpụrụ ndụ ALDH2, nke etinyere na East Asia. Ụdị ALDH2 * 1/* 1 GG nke anụ ọhịa nwere ikike metabolic siri ike, ebe ụdị heterozygous nwere nanị 6% nke ụdị enzyme anụ ọhịa, na ụdị homozygous mutant nwere ihe fọrọ nke nta ka ọ bụrụ ọrụ enzyme efu, na metabolism na-esighi ike na enweghị ike imezu ihe a chọrọ, si otú a na-emerụ ahụ mmadụ.
Ọwa
| FAM | ALDH2 |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | EDTA anticoagulated ọbara |
| CV | <5.0 nke |
| LoD | 103Mpempe akwụkwọ/ml |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®480 ezigbo oge PCR usoro Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Ihe nrụpụta mmịpụta akwadoro: jiri Blood Genome DNA Extraction Kit (DP318) nke Tiangen Biotech (Beijing) Co., Ltd. ma ọ bụ Blood Genome Extraction Kit (A1120) nke Promega wepụta EDTA anticoagulated ọbara Genomic DNA.
Na-atụ aro mmịpụta reagents: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (nke ike ga-eji na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) site Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The mmịpụta ga-eduzi nke ọma dị ka ntuziaka si dị. Olu elution akwadoro bụ100μL.
