Borrelia Burgdorferi Nucleic Acid
Aha ngwaahịa
HWTS-OT068 Borrelia Burgdorferi Nucleic Acid Detection Kit (Fluorescence PCR)
Asambodo
CE
Epidemiology
Ọrịa Lyme na-ebute site na ọrịa Borrelia burgdorferi ma na-ebutekarị ya n'etiti ndị ọbịa anụmanụ, n'etiti anụmanụ ndị ọbịa na ụmụ mmadụ site na akọrọ siri ike.The pathogen Borrelia burgdorferi nwere ike ime ka mmadụ Erythema chronicum migrans, yana ọrịa ndị metụtara ọtụtụ usoro dị ka obi, akwara, na nkwonkwo, wdg, na ngosipụta ụlọ ọgwụ dị iche iche.Dị ka ndị na-emepe emepe nke ọrịa N'ezie, ọ nwere ike kewaa n'oge localized ọrịa, n'etiti disseminated ọrịa na mbubreyo nọgidere na-efe efe, nke bụ oké njọ na ahụ ike nke ndị bi na.Ya mere, na nyocha ụlọ ọgwụ nke Borrelia burgdorferi, ọ dị oke mkpa ịmepụta usoro dị mfe, kpọmkwem na ngwa ngwa maka nchọpụta etiological nke Borrelia burgdorferi.
Ọwa
| FAM | DNA nke Borrelia burgdorferi |
| VIC/HEX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Ihe nlele ọbara dum |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 oyiri/ml |
| Ngwa ndị ọdabara | Sistemụ PCR ABI 7500 ezigbo oge ABI 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
QIAamp DNA Blood Midi Kit nke Qiagen (51185).It kwesiri ibuputan'usoro siri ikena ntụziaka, na akwadoro elution olu bụ100μL.
Nhọrọ 2.
ỌbaraGDNA enomicEngwa ndọpụ (DP318,Mba.: JingchangNdekọ ngwaọrụ20210062) nke Tiangen Biochemical Technology (Beijing) Co., Ltd mepụtara.. It kwesiri ibuputan'usoro siri ikena ntụziaka, na akwadoro elution olu bụ100μL.
Nhọrọ 3.
Ngwa Wizard® Genomic DNA dị ọcha (A1120) nke Promega.It kwesiri ibuputan'usoro siri ikena ntụziaka, na akwadoro elution olu bụ100μL.
