Ngwa Nchọpụta Ọrịa IgM/IgG nke Chikungunya Virus (ọla edo colloidal)
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Ngwa Nchọpụta Ọrịa IgM/IgG nke Chikungunya Virus (ọla edo colloidal)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Chikungunya bụ ọrịa na-efe efe nke nje virus chikungunya (CHIKV) na-akpata, nke anwụnta Aedes na-ebufe, a na-ejikwa ahụ ọkụ, ihe ọkụ na mgbu nkwonkwo mara ya. E gosipụtara na Chikungunya jupụtara na Tanzania na 1952, e kewapụkwara nje ahụ na 1956. Ọrịa a na-efe efe nke ukwuu.n'Africa na Ndịda Ọwụwa Anyanwụ Eshia, ọ kpatakwara nnukwu ọrịa na mpaghara Oké Osimiri India n'afọ ndị na-adịbeghị anya [1]. Nje virus chikungunya nwere otu ụdị ma enwere ike kewaa ya n'ime ụdị mkpụrụ ndụ ihe nketa atọ, ya bụ ụdị West Africa, ụdị Central-East-Southern Africa na ụdị Eshia [2]. Achọpụtala nje Thechikungunya ugbu a n'ihe karịrị mba 110 na Eshia, Afrịka, Yuropu na Amerịka [3]. Ihe mgbaàmà ahụike nke ọrịa ahụ yiri nke ahụ ọkụ dengue, ọ dịkwa mfe ịchọpụta ya nke ọma. Ọ bụ ezie na ọnụọgụ ọnwụ dị obere, ọ dị mfe ịmepụta nnukwu ọrịa na ọrịa na mpaghara nwere oke njupụta anwụnta [4]. IgM na IgG antibodies na ọbara mmadụ dum, serum, ma ọ bụ plasma samples, enwere ike iji ya dị ka ngwa nchọpụta enyemaka maka ọrịa nje chikungunya n'oge na omume ahụike. Ngwa a dabara adaba maka ịchọpụta nje Chikungunyaan na ndị nwere ụfọdụ mgbaàmà
Usoro Nkà na ụzụ
| Nchekwa | 4 ~ 30℃ |
| Ndụ nchekwa | Ọnwa iri abụọ na anọ |
| Ụdị ihe atụ | Ọbara ọbara, plasma, na ihe nlele ọbara dum |
| LoD | Maka nchọpụta IgM: mmetụta uche 97.56%, nkọwapụta98.74%, ngụkọta ọnụego ihe ndabara 98.50%. Maka nchọpụta IgG: mmetụta uche 97.67%, nkọwapụta 98.73%, ọnụego ndakọrịta zuru oke 98.50%. |
| Nkọwapụta | Ahụghị mmeghachi omume ọ bụla na nnwale mmeghachi omume nke ngwa a nanje encephalitis nke Japan, nje encephalitis nke akọrọ na-ebute, ahụ ọkụ siri ike nke nwere nje ọrịa thrombocytopenia, nje ọrịa ahụ ọkụ Xinjiang, Hantavirus, Dengue virus, Hepatitis C virus, Influenza A virus na Influenza Ọrịa nje B. |
Usoro ọrụ:
Biko gụọ akwụkwọ ntuziaka tupu ị nwalee ya nke ọma. Tupu ị nwalee ya, wepụ ihe ndị e ji eme ihe na ihe nlele niile ka e wee nwalee ha site na ọnọdụ nchekwa ma hapụ ha ka ha laghachi na okpomọkụ ụlọ. A ga-eme ule ahụ n'okpomọkụ ụlọ.
Ihe nlele ọbara, plasma, na ọbara dum:
1. Wepụ kaadị nnwale ahụ n'ime akpa foil aluminom wee tinye ya n'elu ala dị larịị ma kpọọ nkụ;
2. Jiri pipette tinye otu ntapụ nke serum, plasma, ma ọ bụ ọbara dum (ihe dị ka 10µL) na "S" nke ihe nlele ahụ;
3. Tinye ntapụ abụọ nke ihe nlele mmiri (ihe dị ka 70 µL) n'ime "S" nke ihe nlele ahụ;
4. Mee mkpebi n'ime nkeji iri na ise ruo iri abụọ. Nsonaazụ egosiri mgbe nkeji iri abụọ gachara adabaghị.
