Chlamydia Trachomatis, Neisseria Gonorrhoeae na Trichomonas vaginalis
Aha ngwaahịa
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae na Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Chlamydia trachomatis (CT) bụ ụdị nje prokaryotic nke na-akpata nje bacteria n'ime mkpụrụ ndụ eukaryotic. A na-ekewa Chlamydia trachomatis n'ime ụdị AK dịka usoro serotype si dị. Ọrịa urogenital tract na-abụkarị site na ụdị trachoma biological variant DK serotypes, a na-egosipụtakarịkwa ụmụ nwoke dị ka urethritis, nke enwere ike iwepụ na-enweghị ọgwụgwọ, mana ọtụtụ n'ime ha na-adịte aka, na-aka njọ mgbe ụfọdụ, enwere ike ijikọ ya na epididymitis, proctitis, wdg.
Ọwa
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Ọgwụgwọ nke trichomonal vaginitis |
| VIC/HEX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | -18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | swab nke akpanwa nwanyị,swab nke nwanyị,Swab nke urethra nwoke |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Mbipụta/mL |
| Nkọwapụta | Enweghị mmeghachi omume na nje ndị ọzọ na-ebute ọrịa STD n'èzí usoro nchọpụta nke ngwa nnwale ahụ, dịka Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, wdg. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1.
Ihe na-eme ka a na-ewepụta ihe: Pipette 1mL nke ihe nlele ahụ a ga-anwale na tube centrifuge DNase/RNase na-enweghị 1.5mL, centrifuge na 12000rpm maka nkeji atọ, tụfuo ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe ghara ịdị irè. Tinye 200µL nke saline nkịtị na ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe ghara ịdị irè ọzọ. Ngwakọta DNA/RNA nke Macro & Micro-Test General (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị ime mwepụ ahụ dịka ntuziaka maka ojiji si dị. Olu ihe nlele ahụ a na-ewepụta bụ 200µL, olu ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe dị nro bụ 80µL.
Nhọrọ nke abụọ.
Ihe a na-atụ aro maka mwepụta: Ngwa mwepụta Nucleic Acid ma ọ bụ Ngwa nhicha (YDP302). A ga-eme mwepụta ahụ nke ọma dịka ntuziaka maka ojiji si dị. Olu mwepụta akwadoro bụ 80µL.
