Chlamydia trachomatis, Neisseria Gonorrhea na Trichomonas vaginalis.
Aha ngwaahịa
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae na Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Chlamydia trachomatis (CT) bụ ụdị prokaryotic microorganism nke na-akpachapụ anya parasitic na sel eukaryotic.Chlamydia trachomatis na-ekewa n'ime AK serotypes dịka usoro serotype siri dị.Ọrịa urogenital tract na-ebutekarị site na trachoma biological variant DK serotypes, na ụmụ nwoke na-egosipụtakarị dị ka urethritis, nke nwere ike ịwepụ ya na-enweghị ọgwụgwọ, ma ọtụtụ n'ime ha na-aghọ nke na-adịghị ala ala, na-akawanye njọ mgbe ụfọdụ, a pụkwara ijikọta ya na epididymitis, proctitis, wdg.
Ọwa
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhea |
| CY5 | Trichomonal vaginitis |
| VIC/HEX | Njikwa ime |
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Ọkpụkpụ cervical nwanyị,Ọkpụkpụ ikpu nwanyị,Nwoke urethra swab |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Mpempe akwụkwọ/ml |
| Nkọwapụta | Ọ dịghị cross-reactivity na ndị ọzọ STD nje pathogens n'èzí nchọpụta nso nke ule kit, dị ka Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus ụdị 1, Herpes simplex virus ụdị 2, Candida albicans, wdg. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Reagement mmịpụta akwadoro: Pipette 1mL nke ihe nlele a ga-anwale ka ọ bụrụ 1.5mL nke DNA centrifuge tube na-enweghị RNase, centrifuge na 12000rpm maka nkeji 3, tụfuo ihe dị elu wee dobe mmiri ozuzo.Tinye 200µL nke saline nkịtị ka ọ na-ebuli elu ka ọ kwụsịlata.Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor). (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị ịme mmịpụta dịka ntuziaka maka ojiji.Olu nlele ewepụtara bụ 200µL, na olu elution akwadoro bụ 80µL.
Nhọrọ 2.
Reagent mmịpụta akwadoro: Mwepụta Nucleic Acid ma ọ bụ Ngwa Ọcha (YDP302).Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka maka ojiji.Olu elution akwadoro bụ 80µL.
