Nje Virus Coxsackie A16 Nucleic Acid

Ngwa a na-anabata Enzymatic Probe Isothermal Amplification (EPIA), ma chepụta primers kpọmkwem na RNA base-nwere nyocha (rProbe) maka mpaghara Cox A16 echekwara nke ọma, ma na-agbakwunye Bst enzyme na RNaseH enzyme n'otu oge, nke aka ekpe na A na-akpọ akụkụ aka nri nke isi RNA nke rProbe na otu fluorescent na quencher, n'otu n'otu.Jiri ọrụ DNA polymerase na ọrụ mbugharị strand nke enzyme Bst iji kwalite ebumnuche a ga-anwale na okpomọkụ na-adị mgbe niile, enzyme RNaseH nwere ike kewaa ntọala RNA na agbụ-nyocha ngwakọ, nke mere na otu fluorescent na onye na-ekpochapụ rProbe bụ. kewapụrụ si otú fluorescing.Na mgbakwunye, enwere ike iji mpempe aka ekpe nke ntọala rProbe RNA residual dị ka primer iji gbasaa n'ihu ịmepụta ngwaahịa, nke na-akwakọba ngwaahịa ahụ.A na-akwakọba mgbaàmà fluorescent na-aga n'ihu site n'ichepụta ngwaahịa ahụ, si otú a na-achọpụta nchọpụta nke nucleic acid.