Nje Virus Dengue, Nje Virus Zika na Chikungunya Virus Multiplex
Aha ngwaahịa
HWTS-FE040 Nje Virus Dengue, Nje Virus Zika na Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ahụ ọkụ dengue (DF), nke ọrịa dengue virus (DENV) na-ebute, bụ otu n'ime ọrịa arbovirus na-efe efe na-efe efe. Usoro mgbasa ya gụnyere Aedes aegypti na Aedes albopictus. DF na-emekarị na mpaghara okpomọkụ na ebe okpomọkụ. DENV bụ nke flavivirus n'okpuru flaviviridae, enwere ike kewaa ya na serotypes 4 dịka antigen elu. Ngosipụta ụlọ ọgwụ nke ọrịa DENV gụnyere isi ọwụwa, ahụ ọkụ, adịghị ike, mmụba nke oghere lymph, leukopenia na ihe ndị ọzọ, na ọbara ọgbụgba, ujo, imeju imeju ma ọ bụ ọbụna ọnwụ n'ọnọdụ ndị siri ike. N'ime afọ ndị na-adịbeghị anya, mgbanwe ihu igwe, ime obodo, mmepe ngwa ngwa nke njem nlegharị anya na ihe ndị ọzọ enyela ngwa ngwa na ọnọdụ dị mma maka mgbasa ozi na ịgbasa DF, na-eduga na mgbasawanye nke mpaghara ọrịa DF mgbe niile.
Ọwa
| FAM | DENV nucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Ọhụrụ ọbara |
| Ct | ≤38 |
| CV | .5% |
| LoD | 500 oyiri/ml |
| Nkọwapụta | Nsonaazụ nnyonye anya na-egosi na mgbe ntinye nke bilirubin na serum na-erughị 168.2μmol / ml, mkpokọta hemoglobin nke hemolysis na-emepụta abụghị ihe karịrị 130g/L, ntinye nke lipid ọbara abụghị ihe karịrị 65mmol/ml, mkpokọta IgG na serum abụghị ihe karịrị 5mg/mL, ọ dịghị ihe na-akpata nje virus dengue ma ọ bụ Zikun. A na-ahọrọ nje ịba ọcha n'anya, nje ịba ọcha n'anya B, nje ịba ọcha n'anya C, nje Herpes, nje virus nke Eastern equine encephalitis, Hantavirus, Bunya virus, West Nile virus na mkpụrụ ndụ ihe nketa mkpụrụ ndụ mmadụ ka a na-ahọrọ maka ule mmeghachi omume cross-reactivity, na nsonaazụ ya gosiri na ọ dịghị mmeghachi omume dị n'etiti ngwa a na ọrịa nje ndị a kpọtụrụ aha n'elu. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge LightCycler®480 ezigbo oge PCR usoro Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
TIANAmp Nje Virus DNA/RNA Kit (YDP315-R), na mmịpụta kwesịrị ịbụ nke e mere n'ụzọ siri ike dabere na ntuziaka maka ojiji. Ihe nlele nke ewepụtara bụ 140μL, na olu elution akwadoro bụ 60μL.
Nhọrọ 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C) na Macro-3006C. Micro-Test Med-Tech Co., Ltd., na mmịpụta kwesịrị ka eme ya dịka ntuziaka maka ojiji. Ihe nlele nke ewepụtara bụ 200μL, na olu elution akwadoro bụ 80μL.
