Nje Vikae, Zika na Chikunya Virusx
Aha
Nje Vikung-Fe040 Durgee, Zika na Chikunya Volumn Nucleic nucleic acid Kit (Fluorescence Pc)
Mpidermiology
Ọrịa DENGEE (DF), nke na-emetụta nje virus (Denv) ọrịa, bụ otu n'ime ọrịa arbovarus na-efe efe. Onye na-ebugharị ya gụnyere Aegas Aegypti na Ages Allopictus. DF bụ ebe a na-ekpo ọkụ na mpaghara ebe okpomọkụ. Denv bụ nke flavivirus n'okpuru flaviviridae, enwere ike ịkepụta ya n'ime 4 cootypes dị ka Antigen dị elu. Ọrịa nke Denv na-ebunye oke isi ọwụwa na-agụnye isi ọwụwa, adịghị ike, agụba na ọgbụgba, mmerụ ahụ ma ọ bụ ọbụna mmerụ ahụ dị njọ. N'afọ ndị na-adịbeghị anya, mgbanwe ihu igwe, mmeju, mmepe nke ihe ndị ọzọ enyerela aka na ọnọdụ ndị ọzọ maka mbugharị na ịgbasa ọdịda DF, na-eduga na mgbasawanye nke DF.
Chaneelu
| M | Denv nucleic acid |
| Rox | Nchịkwa ime |
Nrụta Nka na ụzụ
| Storaji | -18 ℃ |
| Ebe ndu | Ọnwa 9 |
| Ụdị ụdị | Ọhụrụ ọbara |
| Ct | ≤38 |
| CV | <5% |
| Loo | 500 mbipụta / ml |
| Nnoshi | Nsonaazụ Ntụgharị aka na-egosi na mgbe ịta nke biliruubin na ọbara ọ bụghị karịa 138.2μμμ isl intration na-emepụta ihe karịrị 65mmol / ml, mkpokọta IGG itinye uche na Serm abụghị karịa 5mg / ml, enweghị mmetụta na nje virus dinue, nke Zika ma ọ bụ nchọpụta virus Chikungunya. Nje virus, hepatitis b virus, nje herpatis c virus, Hantavianine na-ahọpụta, na-egosi nje virus na-egosi na ọ dịghị Cross Mmeghachi n'etiti Kit na nje ndị a kpọtụrụ aha n'elu. |
| Ngwá agha | Etinyere Biosystems 7500 ezigbo oge pcr sistemụ Ngwa Biosystems na-etinye 7500 ngwa ngwa usoro pcr Nke quagruo®5 ezigbo oge pcr Lightcycler®480 ezigbo oge pcr sistemụ Linegene 9600 Pụnyere Real Chemical System Ma-6000 Real-Oge lyr Beired cfx96 ezigbo oge pcr sistemụ Beired cfx opus 96 Real System |
Na-arụ ọrụ
Nke 1.
TIANPPM virus virus dit / RNA KIT (YDP315-R), a ga-eduzi mmịpụta ya n'ụzọ doro anya na ntuziaka maka iji. Olu nke ewepụtara bụ 140μL, na olu ure a tụrụ aro ya bụ 60μL.
Nke 2.
Macro & Micro-ule General DNA / RWT-3017-50, HWT-3017-28, HWT-3017-97) (nke nwere ike iji ya macro & Micro-3017) (HWT-3006C, HWT-3006B)) site n'aka Jiangsu Macro & Micro-Nnwale Met-Tech Co., ltd., na a ga-eduzi ya dịka ntuziaka si dị. Olu nke eweputara bu 200μL, na olu urora a tụrụ aro bụ 80μL.
