Ọrịa Dengue, Zika Virus na Chikungunya Virus Multiplex
Aha ngwaahịa
Nje Virus HWTS-FE040 Dengue, Zika Virus na Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Dengue (DF), nke ọrịa Dengue virus (DENV) na-akpata, bụ otu n'ime ọrịa ndị na-efe efe kachasị na ọrịa Arbovirus. Usoro mbufe ya gụnyere Aedes aegypti na Aedes albopictus. DF na-adịkarịkarị n'ebe okpomọkụ na mpaghara ndị dị n'okpuru okpomọkụ. DENV bụ nke flavivirus n'okpuru flaviviridae, enwere ike kewaa ya n'ime ụdị anọ dịka antigen dị n'elu si dị. Ngosipụta ahụike nke ọrịa DENV gụnyere isi ọwụwa, ahụ ọkụ, adịghị ike, mmụba nke lymph node, leukopenia na wdg, na ọbara ọgbụgba, ujo, mmerụ ahụ imeju ma ọ bụ ọbụna ọnwụ n'ọnọdụ ndị siri ike. N'afọ ndị na-adịbeghị anya, mgbanwe ihu igwe, mmepe obodo, mmepe ngwa ngwa nke njem nlegharị anya na ihe ndị ọzọ enyela ọnọdụ ngwa ngwa na dị mfe maka izipu na mgbasa nke DF, na-eduga na mmụba mgbe niile nke mpaghara ọrịa DF.
Ọwa
| FAM | Asịd nucleic DENV |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | -18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | Serọm ọhụrụ |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | Mbipụta 500/mL |
| Nkọwapụta | Nsonaazụ nnwale mgbochi na-egosi na mgbe ntinye bilirubin na serum erughị 168.2μmol/ml, ntinye hemoglobin nke hemolysis mepụtara erughị 130g/L, ntinye lipid n'ime ọbara erughị 65mmol/ml, mkpokọta ntinye IgG na serum erughị 5mg/mL, enweghị mmetụta ọ bụla na nje dengue, nje Zika ma ọ bụ nchọpụta nje chikungunya. A na-ahọpụta nje Hepatitis A, nje Hepatitis B, nje Hepatitis C, nje Herpes, nje Eastern equine encephalitis, nje Hantavirus, nje Bunya, nje West Nile na ihe nlele serum genomic mmadụ maka ule cross-reactivity, nsonaazụ ya na-egosikwa na enweghị mmeghachi omume cross n'etiti kit a na nje ndị a kpọtụrụ aha n'elu. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1.
Ngwa DNA/RNA nke nje TIAmp Virus (YDP315-R), a ga-emekwa mwepụ ya dịka ntuziaka maka ojiji si dị. Olu ihe nlele ahụ ewepụtara bụ 140μL, olu mgbapụta ahụ akwadoro bụ 60μL.
Nhọrọ nke abụọ.
Ngwa Nnwale DNA/RNA nke Ngwa ...
