SARS-CoV-2 Spike RBD mgbochi
Aha ngwaahịa
HWTS-RT055A-Enzyme-njikọ Immunosorbent Assay maka ịchọpụta SARS-CoV-2 Spike RBD Antibody
Asambodo
CE
Epidemiology
Ọrịa Coronavirus 2019 (COVID-19) bụ oyi baa n'ihi ọrịa coronavirus ọhụrụ akpọrọ dị ka nnukwu ọrịa iku ume iku ume coronavirus 2 (SARS-CoV-2).SARS-CoV-2 bụ nje dị na nje beta-CoV kpuchiri akụkụ dị okirikiri ma ọ bụ elliptical nwere dayameta ihe dịka 60nm-140nm.COVID-19 bụ ọrịa na-efe efe na-efe efe na-efe efe na-efe efe, ndị mmadụ na-enwekarịkwa ike.Ka ọ dị ugbu a, isi mmalite ọrịa COVID-19 butere ọrịa COVID-19 na onye na-ebu asymptomatic nke SARS-CoV-2.Onu ogugu nke ogwu SARS-CoV-2 butere nwere ike weputa ogwu mgbochi RBD ma obu S antibody nke SARS-CoV-2 nke a na-achọpụta na serum na plasma, nke nwere ike gosiputara iji nyochaa oru oma nke inoculating ogwu SARS-CoV-2.
Nka nka
Nchekwa | 2-8 ℃ |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ụdị ụdị | ọbara mmadụ, plasma, samples nwere anticoagulant nke EDTA, heparin sodium na sodium citrate. |
CV | ≤15.0% |
LoD | Emere ngwa ahụ site na ntụnye aka LOD nke onye nrụpụta yana ọnụego nkwekọrịta 100%. |
Nkọwapụta | Ihe ndị dị elu na-etinye aka na ụdị anaghị emetụta arụmọrụ nke ngwa ahụ maka ịchọpụta SARS-CoV-2 spike RBD mgbochi.Ihe ndị a nwalere na-etinye aka gụnyere haemoglobin (500mg / dL), bilirubin (20mg / dL), triglyceride (1500 mg / dL), heterophil antibody (150U / ml), ihe rheumatoid (100U / ml), 10% (v / v) ọbara mmadụ, phenylephrine (2mg / ml), oxymetazoline (2mg / mL), sodium chloride (ihe nchebe gụnyere) (20mg / ml), beclomethasone (20mg / ml), dexamethasone (20mg / ml), flunisolide (20μg / ml), Triamcinolone (2mg/mL), budesonide (2mg/mL), Mometasone (2mg/mL), fluticasone (2mg/mL), histamine Dihydrochloride (5mg/mL), ainterferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), Peramivir (1mg/mL) lopinavir (500mg/mL), ritonavir (1mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (1 mg/mL). 40μg / ml) na meropenem (200 mg / ml).Levofloxacin (10 μg / ml), tobramycin (0.6mg / ml), EDTA (3mg / ml), Heparin sodium (25U / ml), na sodium citrate (12mg / ml). |
Ngwa ngwa: | Ihe na-agụ microplate zuru ụwa ọnụ na ogologo wavelength 450nm/630nm. |
Usoro ọrụ
Nhọrọ 1.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test akpaka Nucleic Acid Extractor (HWTS-3006).
Nhọrọ 2.
Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech(Beijing) Co., Ltd.