Enterovirus Universal, EV71 na CoxA16
Aha ngwaahịa
HWTS-EV026B-Enterovirus Universal, EV71 na CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-EV020Y/Z-Enterovirus Universal akpọnwụwo, EV71 na CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
Asambodo
CE/MDA (HWTS-EV026)
Epidemiology
Ọrịa ọnụ ụkwụ-ụkwụ (HFMD) bụ ọrịa na-efe efe na-efe efe na ụmụaka. Ọ na-emekarị n'ime ụmụaka na-erubeghị afọ 5, ọ pụkwara ịkpata herpes na aka, ụkwụ, ọnụ na akụkụ ndị ọzọ, na ọnụ ọgụgụ dị nta nke ụmụaka nwere ike ịkpata nsogbu dị ka myocarditis, pulmonary edema, aseptic meningoencephalitis, wdg. Ụmụaka n'otu n'otu nwere ọrịa siri ike na-akawanye ngwa ngwa ma na-adị mfe ịnwụ ma ọ bụrụ na a gwọọghị ya ngwa ngwa.
Ugbu a, a chọtara 108 serotypes nke enteroviruses, nke kewara ụzọ anọ: A, B, C na D. Enteroviruses na-akpata HFMD dị iche iche, ma enterovirus 71 (EV71) na coxsackievirus A16 (CoxA16) bụ ndị kasị nkịtị na na mgbakwunye na HFMD, nwere ike ime ka oké Central ụjọ usoro, na-akpata ọrịa na-adịghị mma, dị ka ọrịa meningitis.
Ọwa
| FAM | Ọrịa enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Njikwa ime |
Nka nka
| Nchekwa | Mmiri: ≤-18℃ N'ọchịchịrịLyophilization: ≤30 ℃ |
| Ndụ nchekwa | Mmiri: ọnwa 9Lyophilization: ọnwa 12 |
| Ụdị ụdị | Akpịrị swab sample, Herpes mmiri mmiri |
| Ct | ≤38 |
| CV | ≤5.0 nkeji |
| LoD | 500 oyiri/ml |
| Ngwa ndị ọdabara | Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa.Sistemụ PCR ABI 7500 ezigbo oge ABI 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time QuantStudio®5 LightCycler®480 PCR ozugbo Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Ngwọta PCR zuru oke
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke ike ga-eji na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) site Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The mmịpụta kwesịrị rụrụ dị ka. Ihe nlele nke ewepụtara bụ 200μL, na olu elution akwadoro bụ 80μL.
Reagent mmịpụta akwadoro: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Ekwesịrị ime mmịpụta ahụ dịka ntuziaka ntuziaka si dị. Ihe nlele nke mmịpụta bụ swabs oropharyngeal ma ọ bụ ihe nlele mmiri herpes nke ndị ọrịa anakọtara na saịtị. Tinye swabs anakọtara ozugbo na Macro & Micro-Test Sample Release Reagent, vortex na mix ọma, tinye n'ime ụlọ okpomọkụ maka nkeji 5, wepụ wee tụgharịa ma gwakọta nke ọma iji nweta RNA nke nlele ọ bụla.
Reagent mmịpụta akwadoro: QIAamp Viral RNA Mini Kit (52904) nke QIAGEN ma ọ bụ Mwepụta Acid Nucleic ma ọ bụ Reagent Ọcha (YDP315-R). Ekwesịrị ime mmịpụta ahụ n'ụzọ siri ike dabere na ntuziaka ntuziaka.
