Enterovirus Universal, EV71 na CoxA16
Aha Ngwaahịa
Ngwa Nchọpụta Acid Nucleic HWTS-EV026B-Enterovirus Universal, EV71 na CoxA16 (Fluorescence PCR)
HWTS-EV020Y/Z-Ngwa Nchọpụta Acid Nucleic nke a kpọrọ nkụ nke a kpọrọ nkụ nke a kpọrọ nkụ nke a kpọrọ nkụ (Fluorescence PCR)
Asambodo
CE/MDA(HWTS-EV026)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa aka-ụkwụ-ọnụ (HFMD) bụ ọrịa na-efe efe nke a na-ahụkarị n'ime ụmụaka. Ọ na-emekarị n'ime ụmụaka na-erubeghị afọ ise, ọ nwekwara ike ibute herpes n'aka, ụkwụ, ọnụ na akụkụ ahụ ndị ọzọ, obere ụmụaka nwekwara ike ibute nsogbu dịka myocarditis, pulmonary edema, aseptic meningoencephalitis, wdg. Ụmụaka nwere ọrịa dị oke njọ na-aka njọ ngwa ngwa ma na-anwụ ma ọ bụrụ na a gwọọghị ha ozugbo.
Ugbu a, achọpụtala ụdị nje enterovirus 108, nke e kewara n'ime otu anọ: A, B, C na D. Enteroviruses ndị na-akpata HFMD dị iche iche, mana enterovirus 71 (EV71) na coxsackievirus A16 (CoxA16) bụ ndị a na-ahụkarị, tinyere HFMD, nwere ike ibute nsogbu siri ike na sistemụ akwara etiti dịka meningitis, encephalitis, na ọrịa mkpọnwụ acute.
Ọwa
| FAM | Ọrịa Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Njikwa dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | Mmiri mmiri: ≤-18℃ N'ọchịchịrịNkwụsị ọrụ: ≤30℃ |
| Ndụ nchekwa | Mmiri mmiri: ọnwa 9Nkwụsị ahụ ọkụ: ọnwa 12 |
| Ụdị ihe atụ | Ihe nlele akpịrị swab, mmiri ọgwụ herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500Mpempe/mL |
| Ngwa Ndị Dị Mkpa | Ọ nwere ike ịha nhata ngwa PCR fluorescent kachasị ewu ewu n'ahịa.Sistemụ PCR ABI 7500 n'oge dị adị Sistemụ PCR ABI 7500 Ngwa Ngwa Ngwa Oge Sistemụ PCR SLAN-96P Oge-N'ezie Sistemụ PCR nke QuantStudio®5 n'oge dị adị Sistemụ PCR nke LightCycler®480 n'oge dị adị Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Ngwọta PCR zuru oke
Ihe na-eme ka a na-ewepụta ihe a tụrụ aro: Ngwakọta Macro & Micro-Test Viral DNA/RNA (HWTS-3017) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. A ga-eme mwepụta ahụ dịka ntuziaka ntuziaka si dị. Olu ihe nlele ewepụtara bụ 200μL, olu mwepụta akwadoro bụ 80μL.
Ihe na-eme ka a na-ewepụta ihe: Ihe na-eme ka a na-ewepụta ihe nlele Macro & Micro-Test (HWTS-3005-8). A ga-eme mwepụ ahụ dịka ntuziaka ntuziaka si dị. Ihe na-eme ka a na-ewepụta ihe nlele ahụ bụ ihe na-eme ka mmiri ọgwụ n'ọnụ maọbụ ihe ndị na-eme ka mmiri ọgwụ herpes dị n'ahụ ndị ọrịa chịkọtara n'ebe ahụ. Tinye ihe ndị a na-anakọta ozugbo na ihe na-eme ka a na-ewepụta ihe nlele Macro & Micro-Test, vortex wee gwakọta ya nke ọma, tinye ya n'ọnọdụ okpomọkụ ụlọ maka nkeji ise, wepụ ya wee tụgharịa ya ma gwakọta ya nke ọma iji nweta RNA nke ihe nlele ọ bụla.
Ihe eji ewepụta ihe a tụrụ aro: QIAamp Viral RNA Mini Kit (52904) nke QIAGEN ma ọ bụ Nucleic Acid Extraction or Purification Reagent (YDP315-R). A ga-eme mwepụ ahụ dịka ntuziaka ntuziaka si dị.
