Nje Virus nke Forest Encephalitis
Aha ngwaahịa
Ngwa Nchọpụta Asịd Nucleic nke Nje Virus HWTS-FE006 Forest Encephalitis (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa encephalitis ọhịa (FE), nke a makwaara dị ka ọrịa encephalitis nke a na-ebute site na tick-borne (TBE), bụ ọrịa na-efe efe nke sistemụ akwara etiti nke nje encephalitis ọhịa na-akpata. Ọrịa encephalitis ọhịa bụ nke ụdị Flavivirus nke ezinụlọ Flaviviridae. Mkpụrụ nje ahụ dị okirikiri ma nwee dayameta nke 40-50nm. Ibu molekul dị ihe dị ka 4×106Da, na mkpụrụ ndụ ihe nketa nje ahụ bụ RNA nwere otu eriri dị mma.[1]N'ọgwụgwọ, a na-amata ya site na oke ahụ ọkụ, isi ọwụwa, nkụchi obi, mmalite ngwa ngwa nke mkpasu iwe meningeal, na mkpọnwụ akwara nke olu na aka na ụkwụ, ọ na-enwekwa oke ọnwụ. Nchọpụta ngwa ngwa na ngwa ngwa nke nje encephalitis ọhịa bụ isi ihe dị mkpa maka ọgwụgwọ nke encephalitis ọhịa, na ịmepụta usoro nchọpụta etiological dị mfe, kpọmkwem na ngwa ngwa dị oke mkpa na nchọpụta ahụike nke encephalitis ọhịa.[1,2].
Ọwa
| FAM | nje virus nke oke ọhịa nke akpọrọ "forest encephalitis" nke akpọrọ nucleic acid |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | serum ọhụrụ |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500Mpempe/mL |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Ihe a na-atụ aro maka mwepụta: Ngwa mwepụta ma ọ bụ ihe nhicha Nucleic Acid (YDP315-R) nke Tiangen Biotech(Beijing) Co., Ltd., a ga-eme mwepụta ahụ dịka ntuziaka si dị. Olu ihe nlele akwadoro bụ 140μL na olu mwepụta akwadoro bụ 60μL.
Ihe na-eme ka a na-ewepụta ihe: Ngwakọta DNA/RNA nke Macro & Micro-Test General (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). A ga-eme mwepụ ahụ dịka ntuziaka si dị. Olu ihe nlele akwadoro bụ 200μL na olu mwepụ ahụ akwadoro bụ 80μL.
