Nje Virus Encephalitis Forest
Aha ngwaahịa
HWTS-FE006 Forest Encephalitis Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Encephalitis ọhịa (FE), nke a makwaara dị ka akọrọ-borne encephalitis (Tick-borne encephalitis, TBE), bụ nnukwu ọrịa na-efe efe nke sistemu ụjọ nke etiti nke nje encephalitis ọhịa kpatara. Nje virus encephalitis ọhịa bụ nke genus Flavivirus nke ezinụlọ Flaviviridae. Ihe nje virus ahụ nwere okirikiri nwere dayameta nke 40-50nm. Arọ molekụla dị ihe dịka 4 × 106Ee, na genome nje bụ ezi uche, RNA nwere otu eriri[1]. N'ụlọ ọgwụ, a na-eji ya ahụ ọkụ dị elu, isi ọwụwa, coma, mgbakasị ahụ nke meningeal ngwa ngwa, na akwara ahụ mkpọnwụ n'olu na akụkụ ahụ, ma nwee ọnụ ọgụgụ ọnwụ dị elu. Nchọpụta nke mbụ na ngwa ngwa nke nje encephalitis ọhịa bụ isi ihe na-agwọ ọrịa encephalitis ọhịa, na nguzobe usoro nyocha nke dị mfe, kpọmkwem na ngwa ngwa etiological dị oke mkpa na nyocha ụlọ ọgwụ nke ọrịa encephalitis ọhịa.[1,2].
Ọwa
| FAM | nje encephalitis ọhịa nucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | ọbara ọhụrụ |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 oyiri/ml |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®480 ezigbo oge PCR usoro Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Na-atụ aro mmịpụta reagent: Nucleic Acid mmịpụta ma ọ bụ Ọcha Kit (YDP315-R) site Tiangen Biotech(Beijing) Co., Ltd., mmịpụta ga-eduzi dị ka ntuziaka siri ike. Olu ihe nlele ewepụtara akwadoro bụ 140μL yana olu elution akwadoro bụ 60μL.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test akpaka Nucleic Acid Extractor (HWTS-6W0-300). HWTS-3006B). a ga-eduzi mmịpụta dịka ntuziaka siri dị. Olu ihe nlele ewepụtara akwadoro bụ 200μL yana olu elution akwadoro bụ 80μL.
