Gardnerella vaginalis nucleic acid
Aha ngwaahịa
HWTS-UR042-Gardnerella Vaginalis Nucleic Acid Nchọpụta Ngwa (Fluorescence PCR)
Epidemiology
Ihe na-ebutekarị vaginitis na ụmụ nwanyị bụ nje vaginosis, na nje bacteria dị mkpa nke nje vaginosis bụ Gardnerella vaginalis. Gardnerella vaginalis (GV) bụ nje na-akpata ohere nke na-adịghị ebute ọrịa mgbe ọ dị obere. Otú ọ dị, mgbe a na-ebelata ma ọ bụ kpochapụ nje bacteria Lactobacilli kasịnụ, na-eme ka ahụ ghara ịdị na mpaghara ikpu, Gardnerella vaginalis na-amụba n'ọtụtụ buru ibu, na-eduga na vaginosis nje. N'otu oge ahụ, nje ndị ọzọ (dị ka Candida, Neisseria gonorrhoeae, Chlamydia trachomatis, wdg) nwere ike ịwakpo ahụ mmadụ, na-ebute vaginitis na cervicitis agwakọta. Ọ bụrụ na achọpụtaghị vaginitis na cervicitis ma gwọọ ya n'oge na nke dị irè, enwere ike inwe ọrịa na-arị elu site na nje ndị na-efe efe n'akụkụ mucosa nke ịmụ nwa, na-eduga n'ụzọ dị mfe na ọrịa traktị dị elu dị ka endometritis, salpingitis, tubo-ovarian abscess (TOA), na pelvic peritonitis, nke nwere ike ịkpata nsogbu dị njọ na ọbụna ime ime.
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | nwoke urethral swab, nwanyị cervical swabs, nwanyị ikpu swab. |
| Ct | ≤38 |
| CV | 5.0% |
| LoD | 400 oyiri/ml |
| Ngwa ndị ọdabara | Ọdabara na ụdị I nchọpụta reagent:Ngwa Biosystem 7500 ezigbo oge PCR, QuantStudio®Sistemụ PCR 5 ezigbo oge, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 PCR ezigbo oge. Ọdabara maka ụdị II reagent nchọpụta: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro ọrụ
Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-8 nwere ike iji E).TM AIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ihe nlele nke ewepụtara bụ 200μL yana olu elution akwadoro bụ 150μL.
