Nje Virus Nucleic
Aha ngwaahịa
Ngwa HWTS-FE005 Hantaan Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Hantavirus bụ ụdị nje RNA kpuchiri ekpuchi, kewara ekewa, na-adịghị mma. A na-ekewa Hantavirus ụzọ abụọ: otu na-ebute ọrịa Hantavirus pulmonary syndrome (HPS), nke ọzọ na-ebute Hantavirus hemorrhagic fever with renal syndrome (HFRS). Nke mbụ bụ nke a na-ahụkarị na Europe na United States, nke ikpeazụ bụ ahụ ọkụ hemorrhagic na ọrịa gbasara akụrụ nke nje Hantaan kpatara, bụ nke a na-ahụkarị na China. Ihe mgbaàmà nke hantavirus hantaan ụdị na-egosipụtakarị dị ka ahụ ọkụ hemorrhagic na ọrịa gbasara akụrụ, nke a na-eji oke ahụ ọkụ, hypotension, ọbara ọgbụgba, oliguria ma ọ bụ polyuria na arụ ọrụ gbasara akụrụ ndị ọzọ. Ọ bụ ọrịa na-efe efe nye ụmụ mmadụ na ekwesịrị ka a kwụọ ya nke ọma.
Ọwa
| FAM | hantavirus hantaan ụdị |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | ọbara ọhụrụ |
| Ct | ≤38 |
| CV | 5.0% |
| LoD | 500 oyiri/μL |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Na-atụ aro mmịpụta reagents: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke ike ga-eji na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) site Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The mmịpụta ga-eduzi dị ka IFU. Olu nke mmịpụta bụ 200 μl. Oke elution akwadoro bụ 80μL.
Reagents mmịpụta akwadoro: Mwepụta Nucleic Acid ma ọ bụ Ngwa Ọcha (YDP315-R). Ekwesịrị ịme mmịpụta ahụ dịka IFU si kwuo. Olu nke mmịpụta bụ 140 μl. Oke elution akwadoro bụ 60μL.
