Ọrịa Hepatitis A
Aha ngwaahịa
Ngwa Nchọpụta Adịgide nke Nje Virus HWTS-HP005 (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Hepatitis A (HAV) bụ isi ihe na-akpata ọrịa hepatitis dị oke njọ. Ọrịa a bụ nje RNA nke nwere otu eriri RNA dị mma ma bụrụkwa nke sitere na ụdị Hepadnavirus nke ezinụlọ Picornaviridae. Ọrịa Hepatitis A, nke a na-ebutekarị site na ụzọ fecal-oral, nke na-eguzogide okpomọkụ, asịd, na ọtụtụ ihe ndị na-agbaze ihe dị ndụ, nwere ike ịdị ndụ ogologo oge na azụ̀ shellfish, mmiri, ala, ma ọ bụ sedimenti dị n'oké osimiri[1-3]. A na-ebute ya site na inwe nri ma ọ bụ mmiri ruru unyi, ma ọ bụ ozugbo ọ na-ebufe ya site na mmadụ gaa na onye. Nri ndị metụtara HAV gụnyere oysters na clams, strọberị, raspberries, blueberries, date, akwụkwọ nri akwụkwọ ndụ akwụkwọ ndụ, na tomato kpọrọ nkụ[4‒6].
Ọwa
| FAM | Asịd nucleic HAV |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Mmiri mmiri: ọnwa 9, Lyophilized: ọnwa 12 |
| Ụdị ihe atụ | Ọbara/nsị |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Mbipụta abụọ/μL |
| Nkọwapụta | Jiri ngwa ndị a nwalee nje ndị ọzọ dịka hepatitis B, C, D, E, enterovirus 71, coxsackie virus, Epstein-Barr virus, norovirus, HIV na genome mmadụ. Enweghị mmeghachi omume cross-reactivity. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR oge-oge LightCycler®480 Sistemụ Nchọpụta PCR LineGene 9600 Plus nke Oge-N'ezie (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Onye Na-agba Okpomọkụ Oge-N'Eziokwu Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Ihe nlele ọbara
Nhọrọ nke 1.
Ngwa Nnwale Ngwa DNA/RNA nke Ngwa Macro na Micro (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). A ga-ewepụta ya dịka ntuziaka si dị. Olu mgbapụta akwadoro bụ 80µL.
Nhọrọ nke abụọ.
Ngwa DNA/RNA nke TIAnamp Virus (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd mepụtara. Ekwesịrị iwepụta ya dịka ntuziaka si dị. Olu ihe nlele ewepụtara bụ 140μL. Olu mmiri akwadoro bụ 60µL.
2.Ihe Nlereanya Ihi Ụkwụ
Ngwa Nnwale Ngwa DNA/RNA nke Ngwa Macro na Micro (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). A ga-ewepụta ya dịka ntuziaka si dị. Olu mgbapụta akwadoro bụ 80µL.
