Ọrịa ịba ọcha n'anya A
Aha ngwaahịa
HWTS-HP005 Hepatitis A Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ọrịa ịba ọcha n'anya A (HAV) bụ isi ihe na-ebute oke ịba ọcha n'anya.Nje virus bụ nje RNA nwere ezi uche nke nwere otu eriri ma bụrụ nke Hepadnavirus genus nke ezinụlọ Picornaviridae.Ọrịa ịba ọcha n'anya, nke a na-ebutekarị site na fecal-oral ụzọ, nke na-eguzogide okpomọkụ, acid, na ọtụtụ ihe mgbaze organic, nwere ike ịdị ndụ ruo ogologo oge na azụ azụ, mmiri, ala, ma ọ bụ sedimenti oke osimiri[1-3].A na-ebute ya site n'inwe nri ma ọ bụ mmiri emetọọ, ma ọ bụ na-ebufe ya ozugbo site na mmadụ gaa na onye.Nri ejikọrọ na HAV gụnyere oporo na clams, strawberries, raspberries, blueberries, date, akwụkwọ ndụ akwụkwọ ndụ akwụkwọ ndụ, na tomato ndị a mịrị amịkpọ [4-6].
Ọwa
| FAM | HAV nucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Mmiri mmiri: ọnwa 9, Lyophilized: ọnwa iri na abụọ |
| Ụdị ụdị | Serum/stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 2 mbipụta/μL |
| Nkọwapụta | Jiri ngwa ahụ nwalee nje ịba ọcha n'anya ndị ọzọ dị ka ịba ọcha n'anya B, C, D, E, enterovirus 71, nje coxsackie, nje Epstein-Barr, norovirus, HIV na genome mmadụ. Ọ dịghị cross-reactivity. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR Real-Time LightCycler®480 LineGene 9600 Plus Real-Time PCR Nchọpụta Sistemụ (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Ihe nlele ọbara
Nhọrọ 1.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ekwesịrị ịwepụta ya dịka ntuziaka ahụ si dị.Olu elution akwadoro bụ 80µL.
Nhọrọ 2.
TIANAmp Virus DNA/RNA Kit (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd rụpụtara. Ekwesịrị ịwepụta ya dịka ntuziaka.Ihe nlele nke ewepụtara bụ 140 μl.Olu elution akwadoro bụ 60µL.
2.Ihe nlele stool
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ekwesịrị ịwepụta ya dịka ntuziaka ahụ si dị.Olu elution akwadoro bụ 80µL.
