Ịba ọcha n'anya B Virus Genotyping

Ihe onyonyo a na-egosi ịba ọcha n'anya B nje Genotyping

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative typing nke ụdị B, ụdị C na ụdị D na nti serum/plasma sample nke ịba ọcha n'anya B nje (HBV)

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-HP002-Ngwunye Nchọpụta Ọrịa ịba ọcha n'anya B (Fluorescent PCR)

Epidemiology

Ka ọ dị ugbu a, a chọpụtala ụdị genotype iri sitere na A ruo J nke HBV n'ụwa niile.HBV genotypes dị iche iche nwere ọdịiche dị na njirimara ọrịa na-efe efe, mgbanwe nje, ngosipụta ọrịa na nzaghachi ọgwụgwọ, wdg, nke ga-emetụta ọnụọgụ HBeAg seroconversion, ịdị njọ nke imeju imeju, na ọrịa cancer imeju ruo n'ókè ụfọdụ, ma na-emetụta ụlọ ọgwụ. prognosis nke ọrịa HBV na ọgwụgwọ ọgwụgwọ nke ọgwụ mgbochi ruo n'ókè ụfọdụ.

Ọwa

ỌwaAha	Nchekwa mmeghachi omume 1	Nchekwa mmeghachi omume 2
FAM	HBV-C	HBV-D
VIC/HEX	HBV-B	Njikwa ime

Nka nka

Nchekwa	≤-18℃ N'ọchịchịrị
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	Plasma, ọbara
Ct	≤38
CV	≤5.0 nkeji
LoD	1 × 10²IU/ml
Nkọwapụta	Ọ dịghị cross-reactivity na ịba ọcha n'anya C, mmadụ cytomegalovirus, Epstein-Barr virus, mmadụ immunodeficiency nje, ịba ọcha n'anya A virus, syphilis, herpes virus, influenza A virus, propionibacterium acnes (PA), wdg.
Ngwa ndị ọdabara	Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. Sistemụ PCR ABI 7500 ezigbo oge ABI 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR ezigbo oge QuantStudio5 LightCycler®480 ezigbo oge PCR Systems Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge