Ịba ọcha n'anya B Virus Genotyping
Aha ngwaahịa
HWTS-HP002-Ngwunye Nchọpụta Ọrịa ịba ọcha n'anya B (Fluorescent PCR)
Epidemiology
Epidemiology
Ọwa
| ỌwaAha | Nchekwa mmeghachi omume 1 | Nchekwa mmeghachi omume 2 |
| FAM | HBV-C | HBV-D |
| VIC/HEX | HBV-B | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ N'ọchịchịrị |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Plasma, ọbara |
| Ct | ≤38 |
| CV | ≤5.0 nkeji |
| LoD | 1 × 102IU/ml |
| Nkọwapụta | Ọ dịghị cross-reactivity na ịba ọcha n'anya C, mmadụ cytomegalovirus, Epstein-Barr virus, mmadụ immunodeficiency virus, ịba ọcha n'anya A virus, syphilis, herpes virus, influenza A virus, propionibacterium acnes (PA), wdg. |
| Ngwa ndị ọdabara | Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. Sistemụ PCR ABI 7500 ezigbo oge ABI 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time QuantStudio®5 LightCycler®480 PCR ozugbo Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) nke nwere ike ji mee ihe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) site Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The mmịpụta ga-nlere dị ka wepụ akwụkwọ ntuziaka ga-nlere dị ka wepụ akwụkwọ ntuziaka na-n'ebe a ga-rụrụ dị ka akwụkwọ ntuziaka.200μL, na olu elution akwadoro bụ80μL.
Reagent mmịpụta akwadoro: Mwepụta Nucleic Acid ma ọ bụ Reagent Ọcha (YDP315). Ekwesịrị ime mmịpụta ahụ n'ụzọ siri ike dabere na ntuziaka. Olu nlele ewepụtara bụ 200µL, na olu elution akwadoro bụ 100µL.
