Ọrịa ịba ọcha n'anya B RNA
Aha ngwaahịa
HWTS-HP007 Ịba ọcha n'anya B Virus RNA Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
QNchọpụta na-adịghị mma nke ọkwa HBV-RNA na serum nwere ike nyochaa ọkwa HBV cccDNA nke ọma na hepatocytes, ma n'otu oge ahụ zere ịkpata mmebi nke ndị ọrịa.Ọ dị oke mkpa maka nchọpụta inyeaka nke ọrịa ịba ọcha n'anya B (HBV) yana nleba anya arụmọrụ yana amụma mwepu ọgwụ nke NAs antiviral therapy na ndị ọrịa CHB.
Ọwa
| FAM | HBV-RNA |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | ọbara |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 100 akwụkwọ/ml |
| Nkọwapụta | Nsonaazụ nnyonye anya na-egosi na ọ nweghị mmetụta na mkpebi siri ike nke HBV RNA mgbe ịta nke bilirubin n'ime ọbara abụghị ihe karịrị 168.2μmol / ml, mkpokọta hemoglobin nke hemolysis mepụtara abụghị ihe karịrị 130g / L, yana ntinye nke lipids ọbara erughị 65 mmol / ml;Enweghị mmetụta ọ bụla na mkpebi siri ike nke HBV RNA mgbe mkpokọta IgG dị na serum abụghị ihe karịrị 5mg/mL.Nsonaazụ ule reactivity na-egosi na ọ nweghị mmeghachi omume dị n'etiti ngwa a na nje virus ndị ọzọ ma ọ bụ nje bacteria nke nucleic acid chọpụtara na nlele ọbara (nje ịba ọcha n'anya C, nje cytomegalovirus mmadụ, nje epstein-barr, nje immunodeficiency mmadụ, nje ịba ọcha n'anya A, syphilis). , ọrịa herpes mmadụ ụdị 6, herpes simplex virus ụdị 1, herpes simplex virus ụdị 2, influenza nje, propionibacterium acnes, staphylococcus aureus, candida albicans) na ụmụ mmadụ genomes;Mgbe ndị ọrịa were lamivudine, telbivudine, adefovir dipivoxil na entecavir, mgbe ibu nje nke ịba ọcha n'anya B n'ime ahụ karịrị LoD kacha nta nke ngwa ahụ, a ka nwere ike ịchọpụta ngwa ahụ nke ọma. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCRNgwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) |
Usoro ọrụ
Nhọrọ 1.
Viral DNA/RNA Isolation Kit (NA007-2) nke GenMag Biotechnology Co., Ltd. Usoro mmịpụta akọwapụtara, biko soro ntuziaka ntuziaka.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor). (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. A ga-eme mmịpụta dị ka ntuziaka ntuziaka si dị, ihe atụpụtara amịpụtara bụ 200μL, na olu elution akwadoro bụ. 80μl.
