Ọrịa ịba ọcha n'anya E
Aha ngwaahịa
HWTS-HP006 Hepatitis E Virus Nchọpụta Nchọpụta Acid (Fluorescence PCR)
Epidemiology
Ọrịa ịba ọcha n'anya E (HEV) bụ nje RNA na-ebute nsogbu ahụike zuru ụwa ọnụ.Ọ nwere nnukwu nnabata nnabata ma nwee akụrụngwa nke ịgafe ihe mgbochi interspecies.Ọ bụ otu n'ime ọrịa nje zoonotic kacha mkpa na-ebute oke mmerụ ahụ mmadụ na anụmanụ.A na-ebunye HEV tumadi site na mbufe fecal-oral, a pụkwara ibunye ya kwụ ọtọ site na embrayo ma ọ bụ ọbara.N'ime ha, n'okporo ụzọ nnyefe nke fecal-oral, mmiri na nri ndị na-emerụ emerụ na-agbasa ebe niile, ihe ize ndụ nke ibute ọrịa HEV na ụmụ mmadụ na anụmanụ dị elu[1-2].
Ọwa
| FAM | HEV nucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Akpịrị swab |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 oyiri/μL |
| Nkọwapụta | Ọrịa ịba ọcha n'anya E (HEV) bụ nje RNA na-ebute nsogbu ahụike zuru ụwa ọnụ.Ọ nwere nnukwu nnabata nnabata ma nwee akụrụngwa nke ịgafe ihe mgbochi interspecies.Ọ bụ otu n'ime ọrịa nje zoonotic kacha mkpa na-ebute oke mmerụ ahụ mmadụ na anụmanụ.A na-ebunye HEV tumadi site na mbufe fecal-oral, a pụkwara ibunye ya kwụ ọtọ site na embrayo ma ọ bụ ọbara.N'ime ha, n'okporo ụzọ nnyefe nke fecal-oral, mmiri na nri ndị na-emerụ emerụ na-agbasa ebe niile, ihe ize ndụ nke ibute ọrịa HEV na ụmụ mmadụ na anụmanụ dị elu[1-2]. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystems 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ekwesịrị ịwepụta ya dịka ntuziaka ahụ si dị.Olu elution akwadoro bụ 80µL.
Nhọrọ 2
TIANAmp Virus DNA/RNA Kit (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd rụpụtara. Ekwesịrị ịwepụta ya nke ọma dịka ntuziaka siri dị.Ihe nlele nke ewepụtara bụ 140 μl.Olu elution akwadoro bụ 60µL.v
