Ọrịa Hepatitis E
Aha ngwaahịa
Ngwa Nchọpụta Asịd Nucleic nke Nje Virus HWTS-HP006 (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Hepatitis E (HEV) bụ nje RNA nke na-akpata nsogbu ahụike zuru ụwa ọnụ. Ọ nwere ọtụtụ ihe mgbochi ma nwee ikike ịgafe ihe mgbochi ụdịdị dị iche iche. Ọ bụ otu n'ime nje ndị kacha mkpa na-efe efe ma na-emerụ mmadụ na anụmanụ ahụ nke ukwuu. A na-ebute HEV site na mbufe fecal-oral, a pụkwara ibufe ya site na embrayo ma ọ bụ ọbara. N'ime ha, n'ụzọ mbufe fecal-oral, mmiri na nri ndị metụtara HEV gbasaara ebe niile, ihe egwu nke ibute HEV n'ime mmadụ na anụmanụ dịkwa elu[1-2].
Ọwa
| FAM | Asịd nucleic HEV |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Swab akpịrị |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Mbipụta 500/μL |
| Nkọwapụta | Ọrịa Hepatitis E (HEV) bụ nje RNA nke na-akpata nsogbu ahụike zuru ụwa ọnụ. Ọ nwere ọtụtụ ihe mgbochi ma nwee ikike ịgafe ihe mgbochi ụdịdị dị iche iche. Ọ bụ otu n'ime nje ndị kacha mkpa na-efe efe ma na-emerụ mmadụ na anụmanụ ahụ nke ukwuu. A na-ebute HEV site na mbufe fecal-oral, a pụkwara ibufe ya site na embrayo ma ọ bụ ọbara. N'ime ha, n'ụzọ mbufe fecal-oral, mmiri na nri ndị metụtara HEV gbasaara ebe niile, ihe egwu nke ibute HEV n'ime mmadụ na anụmanụ dịkwa elu[1-2]. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa Ngwa nke 7500 Ejiri Mee Ihe Sistemụ PCR nke QuantStudio®5 n'oge dị adị Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR oge-oge LightCycler®480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1
Ngwa Nnwale Ngwa DNA/RNA nke Ngwa Macro na Micro (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). A ga-ewepụta ya dịka ntuziaka si dị. Olu mgbapụta akwadoro bụ 80µL.
Nhọrọ nke abụọ
Ngwa DNA/RNA nke TIAnamp Virus (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd mepụtara. Ekwesịrị iwepụta ya nke ọma dịka ntuziaka si dị. Olu ihe nlele ewepụtara bụ 140μL. Olu mmiri akwadoro bụ 60µL.v
