Ụdị nje virus Herpes Simplex 1/2 (HSV1/2) Nucleic Acid

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta in vitro qualitative nke Herpes Simplex Virus Type 1 (HSV1) na Herpes Simplex Virus Type 2 (HSV2) iji nyere aka chọpụta na ịgwọ ndị ọrịa nwere ọrịa HSV a na-enyo enyo.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-UR018A-Herpes simplex nje ụdị 1/2, (HSV1/2) nucleic acid nchọpụta ngwa (Fluorescence PCR)

Epidemiology

Ọrịa ndị a na-ebute site ná mmekọahụ (STD) ka bụ otu n'ime ihe iyi egwu na nchekwa ahụike ọha zuru ụwa ọnụ.Ọrịa ndị dị otú ahụ nwere ike iduga na-amụghị nwa, nwa ebu n'afọ akaghi aka, etuto na nsogbu dị egwu dị iche iche.Enwere ọtụtụ ụdị ọrịa STD, gụnyere nje bacteria, nje, chlamydia, mycoplasma na spirochetes, n'ime ha Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, na Ureaplasma urealyticum na-adịkarị.

Herpes Genital bụ ọrịa a na-ebutekarị site na mmekọahụ site na HSV2, nke na-efe efe nke ukwuu.N'afọ ndị na-adịbeghị anya, ọnụ ọgụgụ nke herpes genital amụbawo nke ukwuu, na n'ihi mmụba nke omume mmekọahụ dị ize ndụ, ọnụọgụ nchọpụta maka HSV1 na herpes genital abawanyela ma kwuo na ọ dị elu dịka 20%-30%.Ọrịa mbụ nke nje herpes genital na-agbachikarị nkịtị na-enweghị akara ngosi ụlọ ọgwụ doro anya ma e wezụga herpes mpaghara na mucosa ma ọ bụ akpụkpọ anụ nke ndị ọrịa ole na ole.Ebe ọ bụ na a na-amata herpes genital site na mwụfu nje na-adị ogologo ndụ na ịdị mfe ịmaliteghachi, ọ dị mkpa iji nyochaa nje ndị ahụ ozugbo enwere ike ma gbochie mgbasa ya.

Ọwa

FAM HSV1
CY5 HSV2
VIC(HEX) Njikwa ime

Nka nka

Nchekwa Mmiri: ≤-18℃ N'ọchịchịrị
Ndụ nchekwa Ọnwa iri na abụọ
Ụdị ụdị ihe nzuzo nke urethra, ihe nzuzo nke cervical
Ct ≤38
CV ≤5.0%
LoD 50 mbipụta/mmeghachi omume
Nkọwapụta Ọ dịghị ihe na-emeghachi omume na ọrịa STD ndị ọzọ dị ka Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, na Ureaplasma urealyticum.
Ngwa ndị ọdabara Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa.

Ngwa Biosystem 7500 ezigbo oge PCR

Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo

QuantStudio®Sistemụ PCR 5 ezigbo oge

Sistemụ PCR SLAN-96P ezigbo oge

LightCycler®Sistemụ PCR 480 ezigbo oge

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Reagent mmịpụta akwadoro: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka ahụ si dị.

Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke nwere ike iji na Macro & Micro-Test). Akpaaka Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị ịme mmịpụta nke ọma dịka ntuziaka ahụ si dị.Olu elution akwadoro kwesịrị ịbụ 80 μL.

Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP315) nke Tiangen Biotech(Beijing) Co., Ltd.Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka ahụ si dị.Olu elution akwadoro kwesịrị ịbụ 80 μL.


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