Ụdị nje virus Herpes Simplex 1/2 (HSV1/2) Nucleic Acid
Aha ngwaahịa
HWTS-UR018A-Herpes simplex nje ụdị 1/2, (HSV1/2) nucleic acid nchọpụta ngwa (Fluorescence PCR)
Epidemiology
Ọrịa ndị a na-ebute site ná mmekọahụ (STD) ka bụ otu n'ime ihe iyi egwu na nchekwa ahụike ọha zuru ụwa ọnụ.Ọrịa ndị dị otú ahụ nwere ike iduga na-amụghị nwa, nwa ebu n'afọ akaghi aka, etuto na nsogbu dị egwu dị iche iche.Enwere ọtụtụ ụdị ọrịa STD, gụnyere nje bacteria, nje, chlamydia, mycoplasma na spirochetes, n'ime ha Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, na Ureaplasma urealyticum na-adịkarị.
Herpes Genital bụ ọrịa a na-ebutekarị site na mmekọahụ site na HSV2, nke na-efe efe nke ukwuu.N'afọ ndị na-adịbeghị anya, ọnụ ọgụgụ nke herpes genital amụbawo nke ukwuu, na n'ihi mmụba nke omume mmekọahụ dị ize ndụ, ọnụọgụ nchọpụta maka HSV1 na herpes genital abawanyela ma kwuo na ọ dị elu dịka 20%-30%.Ọrịa mbụ nke nje herpes genital na-agbachikarị nkịtị na-enweghị akara ngosi ụlọ ọgwụ doro anya ma e wezụga herpes mpaghara na mucosa ma ọ bụ akpụkpọ anụ nke ndị ọrịa ole na ole.Ebe ọ bụ na a na-amata herpes genital site na mwụfu nje na-adị ogologo ndụ na ịdị mfe ịmaliteghachi, ọ dị mkpa iji nyochaa nje ndị ahụ ozugbo enwere ike ma gbochie mgbasa ya.
Ọwa
FAM | HSV1 |
CY5 | HSV2 |
VIC(HEX) | Njikwa ime |
Nka nka
Nchekwa | Mmiri: ≤-18℃ N'ọchịchịrị |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ụdị ụdị | ihe nzuzo nke urethra, ihe nzuzo nke cervical |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 mbipụta/mmeghachi omume |
Nkọwapụta | Ọ dịghị ihe na-emeghachi omume na ọrịa STD ndị ọzọ dị ka Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, na Ureaplasma urealyticum. |
Ngwa ndị ọdabara | Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Reagent mmịpụta akwadoro: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka ahụ si dị.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke nwere ike iji na Macro & Micro-Test). Akpaaka Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị ịme mmịpụta nke ọma dịka ntuziaka ahụ si dị.Olu elution akwadoro kwesịrị ịbụ 80 μL.
Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP315) nke Tiangen Biotech(Beijing) Co., Ltd.Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka ahụ si dị.Olu elution akwadoro kwesịrị ịbụ 80 μL.