Herpes Simplex Nje Virus Ụdị 2 Nucleic Acid

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative nke herpes simplex virus ụdị 2 nucleic acid na nwoke urethral swab na nwanyị cervical swab sample.

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HWTS-UR007A-Herpes Simplex Nje Virus Ụdị 2 Nchọpụta Nucleic Acid (Fluorescence PCR)

Ezubere iji

A na-eji ngwa a maka nchọpụta qualitative nke herpes simplex virus ụdị 2 nucleic acid na nwoke urethral swab na nwanyị cervical swab sample.

Epidemiology

Herpes Simplex Virus Type 2 (HSV2) bụ nje okirikiri arụpụtara ya na tegument, capsid, isi, na envelopu, ma nwee DNA linear nwere eriri abụọ.Nje virus Herpes nwere ike ịbanye n'ime ahụ site na kọntaktị kpọmkwem ma ọ bụ mmekọahụ na akpụkpọ anụ na akpụkpọ anụ mucous, ma kewara ya na nke mbụ na nke na-emekarị.Ọrịa traktị ọmụmụ na-ebutekarị site na HSV2, ndị nwoke na-arịa ọrịa na-egosipụtakwa dị ka ọnya penile, na ndị ọrịa nwanyị na-egosipụtakwa dị ka ọnya cervical, vulvar, na ikpu.Ọrịa mbụ nke nje herpes genital na-abụkarị ọrịa na-ebuteghachi azụ, ma e wezụga maka herpes obodo ole na ole nwere akpụkpọ anụ mucous ma ọ bụ akpụkpọ anụ, nke ọtụtụ n'ime ha enweghị ihe mgbaàmà ụlọ ọgwụ doro anya.Ọrịa herpes genital nwere njiri mara nje na-ebu ogologo ndụ yana ọ dị mfe nlọghachite, ma ndị ọrịa na ndị na-ebu ya bụ isi iyi nke ọrịa ahụ.Na China, ọnụọgụ serological ziri ezi nke HSV2 bụ ihe dịka 10.80% ruo 23.56%.Enwere ike kewaa ọkwa nke ọrịa HSV2 gaa na ọrịa mbụ na ọrịa na-alọghachi azụ, na ihe dị ka pasent 60 nke ndị ọrịa HSV2 butere nlọghachi azụ.

Epidemiology

FAM: Herpes Simplex Nje Virus Ụdị 2 (HSV2) ·

VIC(HEX): Njikwa ime

Ntọala nkwalite PCR

Nzọụkwụ	okirikiri	Okpomọkụ	Oge	AnakọtaFluorescentSignalsma ọ bụ ọ bụghị
1	1 okirikiri	50 ℃	5mins	No
2	1 okirikiri	95 ℃	Nkeji 10	No
3	40 okirikiri	95 ℃	nkeji iri na ise	No
4	40 okirikiri	58 ℃	31 sk	Ee

Nka nka

Nchekwa
Mmiri mmiri	≤-18℃ N'ọchịchịrị
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	Ọkpụkpụ cervical nwanyị, swab urethra nwoke
Ct	≤38
CV	≤5.0%
LoD	50Copy/mmeghachi omume
Nkọwapụta	Ọ dịghị cross-reactivity na ndị ọzọ STD pathogens, dị ka Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium na ndị ọzọ.
Ngwa ndị ọdabara	Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge.