Ngwa Nchọpụta Asịd Nucleic nke Ọrịa Ebola nke Bundybugyo (Fluorescence PCR)
Aha ngwaahịa
Ngwa Nchọpụta Asịd Nucleic nke Ọrịa Ebola nke Bundybugyo (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Filoviridae. Ọ bụ nje Ebola nke atọ nwere ike ibute nnukwu ọrịa na-efe efe nke mmadụ,na-esochi Zaire Ebola virus (EBOV) na Sudan Ebola virus (SUDV). BDBV bụ otu.nje RNA nke eriri na-adịghị mma nwere mkpụrụ ndụ ihe nketa nke ihe dị ka 19 kb, nke na-akọwapụta 7nhaziprotein na protein abụọ na-abụghị nke nhazi. Oge mmalite nke ọrịa BDBV bụ 2–21ụbọchị (nkezi ụbọchị 6.3). Mgbaàmà ya yiri nke nje Ebola ndị ọzọ nke ukwuua pụghịkwa ịmata ọdịiche dị n'etiti ha site na ngosipụta ahụike naanị. Na Ọgọst 2007, otuntiwapụ nke ọrịa ahụ ọkụ ọbara ọgbụgba nke nje virus a na-akọwaghị ihe kpatara ya mere na Bundibugyo District nkeọdịda anyanwụ Uganda. A chọpụtara na ọ bụ nje Ebola ọhụrụ ma kpọọ ya Bundibugyoebolavirus (BDBV). E nwere mmadụ 93 a na-enyo enyo na ha butere ọrịa ahụ, mmadụ 56 enwetara ikpe, na mmadụ 37 nwụrụ.(ọnụọgụgụ ndị nwụrụ anwụ ruru 25%). Na 2012, ọrịa ọzọ mere na Orientale Province nkeDemocratic Republic of Congo, nwere ikpe 38 enwetara na ọnwụ 13 (ọnwụ ikpeọnụego 34%). Na Mee 2026, WHO kwupụtara ọrịa BDBV dị ugbu a na mba ahụ.Mba Democratic Republic of Congo na Uganda bụ Nsogbu Ahụike Ọha nke MbaNchegbu Mba Nile (PHEIC). Ruo Mee 20, e nwere ikpe 246 a na-enyo enyo na ha nwere ikpe 80.ọnwụ.
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | 12ọnwa |
| Ụdị ihe atụ | ọbara dum, serum/plasma,oral swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Mbipụta/mL |
| Ngwa Ndị Dị Mkpa | Ụdị nke Mbụ:Sistemụ PCR ABI 7500/7500 Ngwa ngwa n'oge dị adị, Sistemụ PCR oge QuantStudio®5, Sistemụ PCR oge-oge QuantStudio®7, Sistemụ Nyocha PCR Ahụike Hongshi SLAN-96P/S nke Na-akpaghị Aka, Sistemụ PCR Oge Ntanetị LightCycler®480, Sistemụ PCR oge-oge nke Hangzhou Bioer FQD-96A, Sistemụ PCR oge Yarui MA-6000, Sistemụ PCR oge-oge BioRad CFX96, Sistemụ PCR BioRad CFX Opus 96 n'oge dị adị. Ụdị nke Abụọ: Sistemụ Nchọpụta Molekụla Akpaaka nke Eudemon AIO800 (HWTS-AIO800)
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Usoro ọrụ:
Ụdị I:
Ụdị nke Abụọ:
Ihe ndị na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe ghara ịdị mma: Saline physiological, ihe ndị na-ewepụta ihe na-eme ka a na-akpọ nucleic acid pụta
Ihe oriri ndị a kwadebere onwe ha: Tip paịpụt DNase/RNase na-enweghị, uwe aka a na-atụfu, 1.5 mL
Tubọọlụ centrifuge na-enweghị DNase/RNase, centrifuge
