Ngwa ihe nchọpụta Legionella Pneumophila Nucleic Acid
Aha ngwaahịa
HWTS-RT163-Legionella Pneumophila Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Legionella pneumophila bụ ọkọlọtọ, gram-adịghị mma, obere coccobacillus nke Legionella genus polymorphic. Legionella pneumophila bụ nje nje nje nwere ike ịwakpo amoeba ma ọ bụ macrophages mmadụ. A na-eme ka nje bacteria na-efe efe nke ukwuu na ọnụnọ nke ọgwụ nje na ihe mgbakwunye serum (mana ọnụnọ nke abụọ adịghị mkpa). Legionella pneumophila bụ nje dị mkpa na-akpata ọrịa na-efe efe na oyi baa n'obodo na-enweta na ụlọ ọgwụ na-enweta ihe dịka 80% nke oyi baa Legionella. Legionella pneumophila dị na mmiri na ala. Ịbanye mmiri na ala emetọọ n'ime ahụ mmadụ n'ụdị ikuku ikuku nwere ike ịbụ ụzọ isi ebute ọrịa Legionella. Ka ọ dị ugbu a, ụzọ bụ isi maka nchọpụta ụlọ nyocha na nyocha nke Legionella pneumophila bụ omenala nje na nyocha serological.
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | sputum |
| Ct | ≤38 |
| CV | 5.0% |
| LoD | 1000 oyiri/μL |
| Ngwa ndị ọdabara | Ọdabara na ụdị I nchọpụta reagent: Ngwa Biosystems 7500 Real-Time PCR Systems,QuantStudio®5 Real-Time PCR Systems,SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.),LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioimer0 technology),MA-Time Real-Time Bioimer0 technology,MA-Time Quant-Time Bioimer0. (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. |
| Ọdabara maka ụdị II reagent nchọpụta: EudemonTM AIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro ọrụ
Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-8 nwere ike iji E).TM AIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ihe nlele nke ewepụtara bụ 200μL yana olu elution akwadoro bụ 150μL.
