Middle East Respiratory Syndrome Coronavirus Nucleic Acid

Nkọwa dị mkpirikpi:

A na-eji ngwa ahụ maka nchọpụta qualitative nke MERS coronavirus nucleic acid na nasopharyngeal swabs nwere Middle East Respiratory Syndrome (MERS) coronavirus.

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HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit (Fluorescent PCR)

Epidemiology

Ọrịa iku ume iku ume Middle East coronavirus (MERS-CoV), β-coronavirus na-akpataọrịa iku ume na ụmụ mmadụ, nke mbụ chọpụtara na onye nwụrụ anwụ 60 dị afọ Saudi Arabian nwoke ọrịa na 24th July, 2012. The clinical ngosi nke MERS-CoV ọrịa ranges si asymptomatic ala ma ọ bụ nwayọọ iku ume mgbaàmà na oké nnukwu akụkụ okuku ume ọrịa ọbụna ọnwụ.

Ọwa

FAM	MERS nje virus RNA
VIC(HEX)	Njikwa ime

Nka nka

Nchekwa	≤-18℃ N'ọchịchịrị
Ndụ nchekwa	Ọnwa 9
Ụdị ụdị	swabs nasopharyngeal anakọtara ọhụrụ
CV	≤5.0%
Ct	≤38
LoD	1000 oyiri/ml
Nkọwapụta	Enweghị mmeghachi omume ọ bụla na coronaviruses mmadụ coronavirus SARSr-CoV na nje ndị ọzọ na-ahụkarị.
Ngwa ngwa:	Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Nhọrọ 1.

Reagent mmịpụta akwadoro: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd.

Nhọrọ 2.

Na-atụ aro mmịpụta reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).

Nke gara aga: SARS-CoV-2 dị iche iche

Osote: SARS-CoV-2 IgM/IgG mgbochi

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