Ọrịa Nkuume nke Middle East Coronavirus Nucleic Acid

Foto a egosipụtara maka ọrịa iku ume nke Middle East Coronavirus Nucleic Acid

Nkọwa Dị Mkpirikpi:

A na-eji ngwa a maka ịchọpụta ọnụọgụgụ nke MERS coronavirus nucleic acid na swabs nasopharyngeal nke nwere Middle East Respiratory Syndrome (MERS) coronavirus.

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Nkọwa Ngwaahịa

Akara Ngwaahịa

Aha ngwaahịa

Ngwa Nchọpụta Asịd Nucleic nke HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus (Fluorescent PCR)

Ọmụmụ ihe gbasara ọrịa na-efe efe

coronavirus nke Middle East Respiratory Syndrome coronavirus (MERS-CoV), nke bụ β-coronavirus nke na-akpataA chọpụtara ọrịa iku ume n'ime mmadụ, nke mbụ n'ime otu nwoke Saudi Arabia dị afọ iri isii nwụrụ anwụ na Julaị 24, 2012. Ngosipụta ahụike nke ọrịa MERS-CoV sitere na ọnọdụ enweghị ihe mgbaàmà ma ọ bụ obere ihe mgbaàmà iku ume ruo na ọrịa iku ume dị oke njọ ọbụna ọnwụ.

Ọwa

FAM	RNA nke nje MERS
VIC (HEX)	Njikwa Dị n'ime

Usoro Nkà na ụzụ

Nchekwa	≤-18℃ N'ọchịchịrị
Ndụ nchekwa	Ọnwa itoolu
Ụdị ihe atụ	Swabs nasopharyngeal a chịkọtara ọhụrụ
CV	≤5.0%
Ct	≤38
LoD	1000 Mpịakọta/mL
Nkọwapụta	Enweghị mmeghachi omume na-adịghị mma na coronavirus mmadụ SARSr-CoV na nje ndị ọzọ a na-ahụkarị.
Ngwa Ndị Dị Mkpa:	Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa Sistemụ PCR nke QuantStudio®5 n'oge dị adị Sistemụ PCR SLAN-96P Oge-N'ezie Sistemụ PCR oge-oge LightCycler®480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96

Usoro Ọrụ

Nhọrọ nke 1.

Ihe eji ewepụta ihe a tụrụ aro: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd.

Nhọrọ nke abụọ.

Ihe na-eme ka a na-ewepụta ihe ndị a tụrụ aro: Ngwakọta DNA/RNA nke Macro & Micro-Test General (HWTS-3017) na Ngwakọta Nucleic Acid nke Macro & Micro-Test Automatic (HWTS-3006C, HWTS-3006B).

Nke gara aga: Asịd Nuklia SARS-CoV-2

Osote: Ọgwụ nje SARS-CoV-2 IgM/IgG

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