Nje Virus Monkeypox na Mpempe Nucleic Acid

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative in vitro nke nje clade I, clade II na virus monkeypox universal nucleic acids na mmiri ọkụ ọkụ mmadụ, swabs oropharyngeal na serum sample.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-OT202-Nje Virus Monkeypox na Mpempe Nchọpụta Nucleic Acid (Fluorescence PCR)

Asambodo

CE

Epidemiology

Monkeypox (Mpox) bụ nnukwu ọrịa zoonotic na-efe efe nke Virus Monkeypox (MPXV) kpatara. MPXV bụ okirikiri gbara okirikiri ma ọ bụ oval n'ụdị, ọ bụkwa nje DNA nwere eriri abụọ nwere ogologo ihe dịka 197Kb.[1]. Anụmanụ na-ebutekarị ọrịa a, mmadụ nwekwara ike bute ya site n'ịta anụ ndị bu ọrịa ahụ ma ọ bụ site n'ịmekọrịta ọbara, mmiri ara na ihe ọkụ ọkụ nke anụmanụ ndị butere ya. Enwere ike ibunye nje a n'etiti ndị mmadụ, nke kachasị site na ụmụ irighiri mmiri na-eku ume n'oge ogologo oge, ihu na ihu ma ọ bụ site na kọntaktị kpọmkwem na mmiri ahụ onye ọrịa ma ọ bụ ihe emetọru.[2-3]. Nnyocha egosiwo na MPXV na-emepụta clades abụọ dị iche iche: clade I (nke a na-akpọbu Central African clade ma ọ bụ Congo Basin clade) na clade II (nke a na-akpọbu West African clade). E gosiputara n'ụzọ doro anya na mpox nke Congo Basin clade nwere ike ibufe n'etiti mmadụ ma nwee ike ibute ọnwụ, ebe mpox nke West African clade na-akpata mgbaàmà dị nro ma nwee obere nnyefe mmadụ na mmadụ.[4].

Achọghị nsonaazụ ule nke ngwa a ka ọ bụrụ naanị ihe ngosi maka nchoputa nke ọrịa MPXV na ndị ọrịa, nke a ga-ejikọta ya na njirimara ụlọ ọgwụ nke onye ọrịa na data nyocha ụlọ nyocha ndị ọzọ iji kpee ikpe ziri ezi nke ọrịa nke nje ahụ ma mepụta atụmatụ ọgwụgwọ ezi uche dị na ya iji hụ na ọgwụgwọ dị mma ma dị irè.

Ọwa

FAM MPXV clade II
ROX MPXV Universal nucleic acid
VIC/HEX MPXV clade I
CY5 njikwa ime

Nka nka

Nchekwa

≤-18℃

Ndụ nchekwa Ọnwa iri na abụọ
Ụdị ụdị mmiri ọkụ ọkụ mmadụ, swabs oropharyngeal na serum
Ct ≤38 (FAM, VIC/HEX, ROX), ≤35(IC)
LoD 200 mbipụta/ml
Ngwa ndị ọdabara Ụdị m nchọpụta reagent:

Ngwa Biosystem 7500 ezigbo oge PCR Sistemu

Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo

QuantStudio®Sistemụ PCR 5 ezigbo oge

SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.)

LightCycler®480 ezigbo oge PCR usoro

Sistemụ Nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer)

MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.)

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Ụdị II reagent nchọpụta:

EudemonTMAIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Usoro ọrụ


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