MTHFR Gene Polymorphic Nucleic Acid
Aha ngwaahịa
Ngwa Nchọpụta Acid Nucleic Polymorphic nke Mkpụrụ ndụ HWTS-GE004-MTHFR (ARMS-PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Folic acid bụ vitamin na-agbaze mmiri nke bụ ihe dị mkpa na usoro metabolic nke ahụ. N'afọ ndị na-adịbeghị anya, ọtụtụ nnyocha egosila na mgbanwe nke mkpụrụ ndụ ihe nketa enzyme folate metabolizing MTHFR ga-ebute ụkọ folic acid n'ahụ, mmebi a na-ahụkarị nke enweghị folic acid n'ahụ ndị okenye nwere ike ibute megaloblastic anaemia, mmebi endothelial vascular, wdg. Enweghị folic acid n'ime ụmụ nwanyị dị ime enweghị ike igbo mkpa onwe ha na nwa ebu n'afọ, nke nwere ike ibute ntụpọ tube akwara, anencephaly, ịmụ nwa nwụrụ anwụ, na ime ọpụpụ. Ọkwa folate serum na-emetụta site na polymorphisms 5,10-methylenetetrahydrofolate reductase (MTHFR). Mgbanwe 677C>T na 1298A>C na mkpụrụ ndụ ihe nketa MTHFR na-akpali mgbanwe nke alanine ka ọ bụrụ valine na glutamic acid, n'otu n'otu, na-ebute mbelata ọrụ MTHFR ma si otú a belata ojiji folic acid.
Ọwa
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC (HEX) | Njikwa dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Ọbara EDTA anticoagulated ọhụrụ a chịkọtara |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Ngwa Ndị Dị Mkpa: | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa Sistemụ PCR SLAN ®-96P n'oge a na-arụ ọrụ Sistemụ PCR QuantStudio™ 5 n'oge a Sistemụ PCR oge-oge LightCycler®480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1
Ihe ndị a tụrụ aro maka mwepụta: Ngwa DNA Genomic na Macro & Micro-Test (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Nhọrọ nke abụọ
Ihe ndị a tụrụ aro maka mwepụta: Ngwa Mwepụta DNA nke Blood Genomic (YDP348, JCXB20210062) nke Tiangen Biotech(Beijing) Co.,Ltd. Ngwa Mwepụta Mkpụrụ Ndụ Ọbara (A1120) nke Promega.
