Mycoplasma Hominis Nucleic Acid
Aha ngwaahịa
HWTS-UR004A-Mycoplasma Hominis Nucleic Acid Nchọpụta Ngwa (Fluorescence PCR)
Epidemiology
Ọrịa ndị a na-ebute site ná mmekọahụ (STD) ka bụ otu n'ime ihe ndị dị mkpa na-eyi nchebe ahụike ọha na eze gburugburu ụwa, nke nwere ike iduga na-amụghị nwa, ọmụmụ nwa ebu n'afọ, tumorigenesis na nsogbu dị iche iche dị egwu.Mycoplasma hominis dị na genitourinary tract ma nwee ike ime ka mmeghachi omume mkpasu iwe na tract genitourinary.MH ọrịa nke genitourinary tract nwere ike ịkpata ọrịa ndị dị ka urethritis na-abụghị gonococcal, epididymitis, wdg, na n'etiti ụmụ nwanyị, nke nwere ike ịkpata mbufụt nke usoro ọmụmụ nke na-agbasa na-adabere na cervix.N'otu oge ahụ, ihe mgbagwoju anya nke ọrịa MH bụ salpingitis, na ọnụ ọgụgụ dị nta nke ndị ọrịa nwere ike ịnwe endometritis na ọrịa pelvic inflammatory.
Ọwa
FAM | MH lekwasịrị anya |
VIC(HEX) | Njikwa ime |
Nka nka
Nchekwa | Mmiri: ≤-18℃ N'ọchịchịrị |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ụdị ụdị | ihe nzuzo nke urethra, ihe nzuzo nke cervical |
Ct | ≤38 |
CV | 5.0% |
LoD | 1000 oyiri/ml |
Nkọwapụta | Ọ dịghị cross-reactivity na ndị ọzọ STD nje pathogens, nke dị n'èzí nchọpụta nso, na ọ dịghị cross-reactivity na chlamydia trachomatis, ureaplasma urealyticum, neisseria gonorrhoeae, mycoplasma genitalium, herpes simplex virus ụdị 1, herpes simplex virus ụdị 2. , wdg. |
Ngwa ndị ọdabara | Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Reagent mmịpụta akwadoro: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ekwesịrị ịme mmịpụta ahụ n'ụzọ siri ike dabere na ntuziaka.
Nhọrọ 2.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke nwere ike iji na Macro & Micro-Test). Akpaaka Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị ịme mmịpụta nke ọma dịka ntuziaka ahụ si dị.Olu elution akwadoro kwesịrị ịbụ 80 μL.
Nhọrọ 3.
Na-atụ aro mmịpụta reagent: Nucleic Acid mmịpụta ma ọ bụ Purification Reagent (YDP302) site Tiangen Biotech (Beijing) Co., Ltd.. The mmịpụta kwesịrị-eduzi na-usoro siri na ntuziaka na-atụ aro elution olu bụ 80µL.