Kemgbe Mee 2022, a kọwapụtala ikpe mpox n'ọtụtụ mba na-adịghị agbasa n'ụwa site na mgbasa ozi obodo.
Na 26 Ọgọst, Healthtù Ahụ Ike (wa (WHO) weputara mba ụwaAtụmatụ Nkwadebe na Nzaghachi Atụmatụiji kwụsị mgbasa nke mpox mmadụ na mmadụ site n'usoro ejikọtara ọnụ zuru ụwa ọnụ, mpaghara na mba. Nke a na-esote nkwupụta mberede ahụike ọha na eze nke nchegbu mba ụwa nke Onye isi oche WHO na 14 Ọgọst.
Okwesiri iburu n'uche na ntiwapụ nke mpox oge a dị iche na nke 2022, bụ nke na-agbasa n'etiti ndị nwoke na-enwe mmekọahụ, na ọnụ ọgụgụ ndị butere ọrịa na-anwụ ihe na-erughị 1%.
Ụdi nke juru ebe niile na nso nso a "Clade Ib", nke bụ ihe dị iche iche nke Clade I, nwere ọnụ ọgụgụ ọnwụ dị elu. Ọdịiche ọhụrụ a malitere ịgbasa na DRC na Septemba gara aga, na mbụ n'etiti ndị na-eme mmekọahụ, ma gbasaa ugbu a na otu ndị ọzọ, ebe ụmụaka na-enwekarị ike.
Africa CDC kwuru n'ime akụkọ n'ọnwa gara aga na a chọpụtala ọrịa mpox na mba 10 n'Africa n'afọ a, gụnyere DRC, nke gosipụtara 96.3% nke ikpe niile n'Africa n'afọ a yana 97% nke ọnwụ. Ọ dị mma ịmara na ihe fọrọ nke nta ka ọ bụrụ 70% nke ikpe na DRC bụ ụmụaka na-erubeghị afọ 15, otu a na-akpata 85% nke ọnwụ na mba ahụ.
Mpox bụ zoonosis nke nje mpox na-ebute na oge nnabata nke ụbọchị 5 ruo 21, nke kariri ụbọchị 6 ruo 13. Onye bu ọrịa ahụ ga-enwe mgbaàmà dị ka ahụ ọkụ, isi ọwụwa na ọzịza nke lymph nodes, nke na-esote ya n'ihu na akụkụ ahụ ndị ọzọ, nke na-eji nwayọọ nwayọọ ghọọ pustules na-ewe ihe dị ka otu izu tupu akpụcha ya. Ikpe ahụ na-efe efe site na mmalite nke mgbaàmà ruo mgbe akpụkpa ahụ daa n'ụzọ nkịtị.
Macro & Micro-Test na-enye ule ngwa ngwa, ngwa ihe omimi na usoro nhazi usoro maka nchọpụta nje mpox, na-enyere aka ịchọpụta nje mpox n'ime oge, nlekọta nke mmalite ya, usoro ọmụmụ ya, nnyefe na ụdị genomic:
Ọrịa Monkeypox AntigenNgwa nchọpụta (Immunochromatography)
Nlele dị mfe (mmiri ọkụ ọkụ / akpịrị akpịrị) na nsonaazụ ngwa ngwa n'ime 10-15 min;
Mmetụta dị elu na LoD nke 20pg / ml na-ekpuchi Clade I & II;
Nkọwa dị elu na-enweghị mmeghachi omume na-akpata ọrịa kịtịkpa, nje varicella zoster, nje rubella, nje herpes simplex, wdg.
OPA nke 96.4% tụnyere NAAT;
Ngwa sara mbara dị ka kọstọm, CDC, ụlọ ahịa ọgwụ, ụlọ ọgwụ, ụlọ ọgwụ ma ọ bụ n'ụlọ.
Enwe-virus IgM/IgG Antibody Detection Kit(Immunochromatographhy)
Arụ ọrụ na-enweghị ngwa ngwa na nsonaazụ ngwa ngwa n'ime 10 min;
Mmetụta dị elu na nkọwapụta nke na-ekpuchi Clade I & II;
Na-achọpụta IgM na IgG ikpebi ọkwa ọrịa mpox;
Ngwa sara mbara dị ka kọstọm, CDC, ụlọ ahịa ọgwụ, ụlọ ọgwụ, ụlọ ọgwụ ma ọ bụ n'ụlọ;
Kwesịrị ekwesị maka nnukwu nyocha nke ọrịa mpox a na-enyo enyo.
Ihe nchọpụta Nucleic Acid Virus Monkeypox (Enzymatic Probe Isothermal Amplification)
Mmetụta dị elu na LoD nke 200 Copy/mL nwere IC, hà nhata florescence PCR;
Arụ ọrụ dị mfe: lysed sample agbakwunyere na lyophilized reagent tube maka nkwalite na-achọ ozugbo enyere site na modul nwere onwe ya nke Easy Amp System;
Nkọwa dị elu na-enweghị mmeghachi omume cross na nje kịtịkpa, nje vaccinia, nje cowpox, nje mousepox, nje virus herpes simplex, nje varicella-zoster, na genome mmadụ, wdg;
Nlele dị mfe (mmiri ọkụ ọkụ / oropharyngeal swab) na nsonaazụ dị mma ngwa ngwa n'ime 5 min;
Ezigbo ụlọ ọgwụ na-ekpuchi Clade I & II na PPA nke 100%, NPA nke 100%, OPA nke 100% na Kappa Value nke 1.000 ma e jiri ya tụnyere Fluorescence PCR kit;
Ụdị Lyophilized chọrọ naanị ibuga na nchekwa okpomọkụ na-enye ohere ịnweta na mpaghara niile;
Ọnọdụ na-agbanwe agbanwe na ụlọ ọgwụ, ebe nlekọta ahụike, yana Easy Amp maka ịchọpụta ihe achọrọ;
Ihe nchọpụta Nucleic Acid Virus Monkeypox (Fluorescence PCR)
Mkpụrụ ndụ abụọ edoro anya na uche dị elu na LoD nke 200 mbipụta / ml;
Nlele mgbanwe nke mmiri ọkụ ọkụ, akpịrị akpịrị na serum;
Nkọwa dị elu na-enweghị mmeghachi omume cross na nje kịtịkpa, nje vaccinia, nje cowpox, nje mousepox, nje virus herpes simplex, nje varicella-zoster, na genome mmadụ, wdg;
Mfe ọrụ: ngwa ngwa sample lysis site sample ntọhapụ reagent na-agbakwunyere na mmeghachi omume tube;
Nchọpụta ngwa ngwa: nsonaazụ n'ime 40 min;
Achọpụtara nke ọma site na njikwa ime na-ahụ maka usoro nchọpụta niile;
Ọmarịcha arụmọrụ ụlọ ọgwụ na-ekpuchi Clade I & II na PPA nke 100%, NPA nke 99.40%, OPA nke 99.64% na Kappa Uru nke 0.9923 ma e jiri ya tụnyere usoro;
Ụdị Lyophilized chọrọ naanị ibuga na nchekwa okpomọkụ na-enye ohere ịnweta na mpaghara niile;
Dakọtara na sistemu Fluorescence PCR nke bụ isi;
Ọnọdụ mgbanwe maka ụlọ ọgwụ, CDC na ụlọ nyocha;
Nje Virus Orthopox Universal Ụdị/Ngwunye Nchọpụta Nucleic Acid (Fluorescence PCR)
mkpuchi zuru oke: na-anwale nje virus 4 orthopox niile nke nwere ike ibunye mmadụ na mpox juru ebe niile (Clade I&II gụnyere) na otu ule iji zere nchọpụta efu;
Mmetụta dị elu na LoD nke 200 mbipụta / ml;
Nkọwa dị elu na-enweghị nzaghachi na-emeghachi omume na nje ndị ọzọ na-akpata rashes dị ka nje virus herpes simplex, varicella-zoster virus, na genome mmadụ, wdg;
Mfe ọrụ: ngwa ngwa sample lysis site sample ntọhapụ reagent ka agbakwunyere na otu tube mmeghachi omume nchekwa;
Nchọpụta ngwa ngwa: mmụba ngwa ngwa na nsonaazụ n'ime 40 min;
Achọpụtara nke ọma site na njikwa ime na-ahụ maka usoro nchọpụta niile;
Dakọtara na sistemu Fluorescence PCR nke bụ isi;
Ọnọdụ mgbanwe maka ụlọ ọgwụ, CDC na ụlọ nyocha;
Ọrịa enweVirus TypingNucleicAcidDechicheKya (Fluorescence PCR)
N'otu oge na-akọwapụta Clade I na Clade II, nke dị mkpa maka ịghọta njirimara ọrịa na-efe efe nke nje a, ịchọpụta mgbasa ya, na imepụta usoro mgbochi na njikwa ezubere iche.
Mmetụta dị elu na LoD nke 200 mbipụta / ml;
Nleba anya na-agbanwe agbanwe nke mmiri ọkụ ọkụ, swab oropharyngeal na serum;
Nkọwa dị elu na-enweghị mmeghachi omume n'etiti Clade I na II, nje ndị ọzọ na-akpata rashes dị ka nje virus herpes simplex, nje varicella-zoster, na genome mmadụ, wdg;
Mfe ọrụ: ngwa ngwa sample lysis site sample ntọhapụ reagent ka agbakwunyere na otu tube mmeghachi omume nchekwa;
Nchọpụta ngwa ngwa: nsonaazụ n'ime 40 min;
Achọpụtara nke ọma site na njikwa ime na-ahụ maka usoro nchọpụta niile;
Ụdị Lyophilized chọrọ naanị ibuga na nchekwa okpomọkụ na-enye ohere ịnweta na mpaghara niile;
Dakọtara na sistemu Fluorescence PCR nke bụ isi;
Ọnọdụ mgbanwe maka ụlọ ọgwụ, CDC na ụlọ nyocha;
Enwe Nje Virus Universal dum GenomeNchọpụtaKit (Multi-PCR NGS)
Ngwa Monkeypox Virus Whole Genome Detection Kit nke Macro & Micro-Test emepụtara ọhụrụ maka ọnọdụ dị iche iche, jikọtara ya na usoro nanopore ONT, nwere ike nweta MPXV usoro genome dum yana mkpuchi na-erughị 98% n'ime awa 8.
Ọ dị mfe ịrụ ọrụ: teknụzụ nkwalite otu nzọụkwụ nwere ikike, enwere ike nweta usoro genome niile nke nje mpox site na nkwalite otu okirikiri;
Mmetụta na nke ziri ezi: na-achọpụta ihe nlele dị ala na 32CT, na 600bp amplicon nanopore sequencing nwere ike izute mgbakọ genome dị elu;
Ultra-ngwa ngwa: ONT nwere ike mezue mgbakọ genome n'ime awa 6-8;
Ndakọrịta zuru oke: ya na ONT, Qi Carbon, SALUS Pro, lllumina, MGI na ndị ọzọ 2 isi.ndna 3rdọgbọ usoro.
Ultra-enwe mmetụtaNje Virus Monkey dumNchọpụtaKit-Ilumina/MGI(Multi-PCR NGS)
Banyere ọnụ ọgụgụ buru ibu nke 2 dị adịndọgbọ usoro n'ụwa nile, Macro & Micro-Test ewepụtala ultra-sensitive kits adapting ka mainstream sequencers iji nweta ala-concentration sample viral genome sequencing;
Ngosipụta nke ọma: 1448 ụzọ abụọ nke 200bp amplicon ultra-dense primer imewe maka nrụpụta nkwalite dị elu na mkpuchi otu;
Ịrụ ọrụ dị mfe: Enwere ike nweta ọbá akwụkwọ Mpox virus lumina/MGI site na mmụba gburugburu abụọ n'ime awa 4, na-ezere usoro iwu ụlọ akwụkwọ dị mgbagwoju anya na ụgwọ reagent;
Mmetụta dị elu: na-achọpụta ihe nlele dị ala ruo 35CT, na-ezere nsonaazụ na-adịghị mma nke ọma site na mmebi iberibe ma ọ bụ ọnụ ọgụgụ dị ala;
Ndakọrịta sara mbara na mainstream 2ndusoro ọgbọ dịka lllumina, Salus Pro ma ọ bụ MGI;Ruo ugbu a, agwụla ihe karịrị 400 ụlọ ọgwụ.
Oge nzipu: Ọgọst-28-2024