Otu B Streptococcus nucleic acid nchọpụta ngwa (Enzymatic Probe Isothermal Amplification)
1.Detection pụtara
Otu B streptococcus (GBS) bụ nke a na-achịkwakarị n'ime ikpu na ikensi ụmụ nwanyị, nke nwere ike ibute ọrịa mbufụt n'oge (GBS-EOS) n'ime ụmụ amụrụ ọhụrụ site na nnyefe kwụ ọtọ site na nne gaa n' nwa, ọ bụkwa isi ihe na-ebute ọrịa oyi baa ọhụrụ, meningitis, septicemia na ọbụna ọnwụ. N'afọ 2021, nkwenye ndị ọkachamara na nlekọta ụlọ ọgwụ nke ụmụ amụrụ ọhụrụ nwere nnukwu ihe ize ndụ nwere ọrịa mmalite mmalite n'otu ọnụ ụlọ nke ndị nne na ụmụ ọhụrụ nke China Maternal and Child Health Association tụrụ aro na nyocha GBS 35-37 izu tupu nnyefe na mgbochi intrapartum ọgwụ nje (IAP) bụ usoro dị irè iji gbochie GBS-EOS n'ime ụmụ ọhụrụ.
2. Ihe ịma aka chere ụzọ nchọpụta ugbu a ihu
Mgbawa nke membranes (PROM) na-aga n'ihu na-ezo aka na mgbawa nke membranes tupu arụ ọrụ, nke bụ ihe mgbagwoju anya na-emekarị n'oge ime nwa. Mgbawa nke akpụkpọ ahụ n'oge na-adịbeghị anya N'ihi mgbawa nke akpụkpọ ahụ, GBS dị na ikpu nke ụmụ nwanyị na-enwe mmekọahụ na-agbasawanye elu, na-ebute ọrịa intrauterine. Ihe ize ndụ nke ibute ọrịa bụ n'ụzọ ziri ezi na uto n'oge mgbawa nke membranes (> 50% nke ndị inyom dị ime na-amụ nwa n'ime 1-2h awa mgbe mgbawa nke membranes, ma ọ bụ ọbụna 1-2 awa).
Ụzọ nchọpụta ndị dị ugbu a enweghị ike imezu ihe achọrọ nke oge (<1h), izi ezi na nchọta GBS na-akpọ oku n'oge nnyefe.
| Ngwa nchọpụta | Omenala nje bacteria | Oge omenala: 18-24hỌ bụrụ na nnwale nnabata ọgwụ: mụbaa 8-16h | 60 % ezigbo nchọpụta ọnụọgụ ; N'oge usoro nlele, ọ na-enwe ike ibute nje bacteria dị ka Enterococcus faecalis gburugburu ikpu na ike, na-ebute nsonaazụ ọjọọ / ụgha ụgha. |
| Immunochromatography | Oge nchọpụta: 15min. | Mmetụta ahụ dị ala, ọ dịkwa mfe ịchọta nchọpụta, karịsịa mgbe ọnụọgụ nje bacteria dị ntakịrị, ọ na-esiri ike ịchọpụta, na ụkpụrụ nduzi adịghị akwado ya. | |
| PCR | Oge nchọpụta: 2-3h | Oge nchọpụta bụ ihe karịrị awa 2, na ngwaọrụ PCR kwesịrị ka anwale ya na batches, ma ọ gaghị ekwe omume ịgbaso ule ahụ. |
3. Macro & Micro-Test ngwaahịa pụta ìhè
Nchọpụta ngwa ngwa: N'iji usoro nyocha mgbaze enzyme akwadoro na usoro mmụba okpomọkụ mgbe niile, ndị ọrịa nwere ike ịmata nsonaazụ ya ozugbo nkeji ise.
Nchọpụta n'oge ọ bụla, ọ dịghị mkpa ichere: ọ na-onwem na okpomọkụ mgbe nile nucleic acid amplification analyzer Easy Amp, na anọ modul na-agba ọsọ onwe ha, na samples na-enyocha ka ha na-abata, n'ihi ya, ọ dịghị mkpa ichere maka samples.
Ụdị ihe atụ dị iche iche: enwere ike ịchọta swab swab, rectal swab ma ọ bụ agwakọta swab, nke na-ezute nkwenye nke ụkpụrụ GBS, na-eme ka ọnụọgụ nchọpụta dị mma ma na-ebelata ọnụ ọgụgụ na-ezighị ezi.
Ọmarịcha arụmọrụ: nnukwu ihe nlele nyocha ụlọ ọgwụ (> ikpe 1000), uche 100%, nkọwapụta 100%.
Mepee reagent: dakọtara na ngwa PCR dị ugbu a nke fluorescence quantitative.
4. Ngwaahịa ozi
| Nọmba ngwaahịa | Aha ngwaahịa | Nkọwapụta | Nọmba akwụkwọ ndebanye aha |
| HWTS-UR033C | Otu B Streptococcus nucleic acid ngwa nchọpụta (Enzymatic Probe Isothermal Amplification) | 50 ule/kit | Ndebanye aha igwe igwe China 20243400248 |
| HWTS-EQ008 | Sistemụ Nchọpụta Isothermal dị mfe Amp Real-time Fluorescence | HWTS-1600P (4 ọwa) | Ndebanye aha igwe igwe China 20233222059 |
| HWTS-1600S (2 ọwa) | |||
| HWTS-EQ009 |
Oge nzipu: Mar-07-2024