Ngwa nchọpụta Streptococcus nucleic acid nke otu B (Enzymatic Probe Isothermal Amplification)
1. Nchọpụta dị mkpa
A na-ahụkarị nje streptococcus Group B (GBS) n'ime ikpu na rectum ụmụ nwanyị, nke nwere ike ibute ọrịa mbuso agha n'oge (GBS-EOS) n'ime ụmụ amụrụ ọhụrụ site na nnyefe kwụ ọtọ site na nne ruo na nwa, ọ bụkwa isi ihe na-akpata oyi baa n'ahụ ụmụ amụrụ ọhụrụ, meningitis, septicemia na ọbụna ọnwụ. Na 2021, Nkwekọrịta Ọkachamara na Njikwa Ahụike nke Ụmụ amụrụ ọhụrụ dị oke ize ndụ nwere ọrịa mbido n'otu ọnụ ụlọ nke Ndị Nne na Ụmụ Infants nke China Maternal and Child Health Association tụrụ aro na nyocha GBS izu 35-37 tupu a mụọ nwa na mgbochi ọgwụ nje intrapartum (IAP) bụ usoro dị irè iji gbochie GBS-EOS n'ime ụmụ amụrụ ọhụrụ.
2. Ihe ịma aka ndị usoro nchọpụta dị ugbu a na-eche ihu
Mgbawa akpụkpọ ahụ tupu oge eruo (PROM) na-ezo aka na mgbawa akpụkpọ ahụ tupu oge eruo, nke bụ nsogbu a na-ahụkarị n'oge ọmụmụ nwa. Mgbawa akpụkpọ ahụ tupu oge eruo N'ihi mgbawa akpụkpọ ahụ, GBS n'ime ikpu ụmụ nwanyị na-amụ nwa nwere ike ịgbasa elu, na-ebute ọrịa intrauterine. Ihe ize ndụ nke ibute ọrịa dị kpọmkwem na uto n'oge mgbawa akpụkpọ ahụ (ihe karịrị 50% nke ụmụ nwanyị dị ime na-amụ nwa n'ime awa 1-2 mgbe akpụkpọ ahụ gbawara, ma ọ bụ ọbụna awa 1-2).
Usoro nchọpụta dị ugbu a enweghị ike imezu ihe achọrọ maka oge (< 1h), izi ezi na nchọpụta GBS n'oge a na-ebuga ya.
| Ngwa nchọpụta | Omenala nje bacteria | Oge Omenala: awa 18-24Ọ bụrụ na nnwale mmetụta ọgwụ ahụ: mụbaa awa 8-16 | Ọkwa nchọpụta dị mma nke pasentị 60; n'oge a na-enyocha ihe nlele ahụ, ọ na-adị mfe ịnweta nje bacteria dịka Enterococcus faecalis gburugburu ikpu na ike, na-ebute nsonaazụ ụgha na-adịghị mma / ụgha. |
| Immunochromatography | Oge nchọpụta: nkeji iri na ise. | Mmetụta ahụ dị obere, ọ dịkwa mfe ịmata ihe ọ na-eme, ọkachasị mgbe ọnụọgụ nje bacteria dị obere, ọ na-esiri ike ịchọpụta ya, a naghịkwa atụ aro ka e nye ntuziaka ndị a. | |
| PCR | Oge nchọpụta: awa 2-3 | Oge nchọpụta ahụ karịrị awa abụọ, a ga-anwale ngwa PCR n'otu n'otu, ọ gaghịkwa ekwe omume ịgbaso ule ahụ. |
3. Isi ihe dị mkpa na ngwaahịa Macro na Micro-Test
Nchọpụta ngwa ngwa: Site na iji usoro nyocha enzyme patented digestion probe na-agbanwe agbanwe, ndị ọrịa nwere ezi ahụ nwere ike ịmata nsonaazụ ya ozugbo nkeji ise.
Nchọpụta oge ọ bụla, ọ dịghị mkpa ichere: e nwere ihe nyocha olu nucleic acid nke na-agbanwe agbanwe nke na-arụ ọrụ n'otu oge, Easy Amp, modulu anọ ahụ na-arụkwa ọrụ n'onwe ha, a na-enyochakwa ihe nlele ndị ahụ ka ha na-abata, yabụ ọ dịghị mkpa ichere ihe nlele.
Ụdị ihe nlele dị iche iche: swab ikpu, swab rectal ma ọ bụ swab ikpu agwakọtara agwakọta nwere ike ịchọpụta, nke na-emezu ndụmọdụ nke ntuziaka GBS, na-eme ka ọnụego nchọpụta dị mma ka mma ma na-ebelata ọnụego nchọpụta na-ezighi ezi.
Arụmọrụ dị mma: nkwenye ahụike dị ukwuu nke ọtụtụ ebe (> ikpe 1000), mmetụta uche 100%, nkọwapụta 100%.
Ihe na-emeghe: dakọtara na ngwa PCR nke na-emepụta fluorescence quantitative dị ugbu a.
4. Ozi ngwaahịa
| Nọmba Ngwaahịa | Aha Ngwaahịa | Nkọwapụta | Nọmba asambodo ndebanye aha |
| HWTS-UR033C | Ngwa nchọpụta asịd Streptococcus nucleic nke otu B (Ịkwalite Ọkwa Isothermal nke Enzymatic Probe) | Nnwale 50/ngwa | Ndebanye aha igwe China 20243400248 |
| HWTS-EQ008 | Sistemụ Nchọpụta Isothermal Fluorescence dị mfe nke oge a na-akpọ Easy Amp | HWTS-1600P(Ọwa anọ) | Ndebanye aha igwe China 20233222059 |
| HWTS-1600S(ọwa abụọ) | |||
| HWTS-EQ009 |
Oge ozi: Maachị-07-2024