Ngwa ọgụ ọhụrụ maka nchọpụta ụkwara nta na nchọpụta mgbochi ọgwụ: Usoro nke ezubere iche nke ọgbọ ọhụrụ (tNGS) jikọtara ya na mmụta igwe maka ọrịa ụkwara nta na nchọpụta hypersensitivity.
Akuko akwukwo: CCa: ihe nleba anya nyocha nke dabere na tNGS na mmụta igwe, nke dabara ndi mmadu nwere obere ukwara nta nke nje na ụkwara nta maningitis.
Thesis title: Tuberculous-ezubere iche ọgbọ na-abịa usoro usoro na igwe mmụta igwe: atụmatụ ultra-sensitive nchọpụta nchọpụta maka paucific pulmonary tubulars na tubular meningitis.
Oge: 《Clinica Chimica Acta》
Ọ bụrụ: 6.5
Ụbọchị mbipụta: Jenụwarị 2024
Ejikọtara ya na Mahadum nke Chinese Academy of Sciences na Beijing Chest Hospital nke Capital Medical University, Macro & Micro-Test guzosie ike a ụkwara nta nchoputa nlereanya dabere na ọgbọ ọhụrụ nke ezubere iche sequencing (tNGS) technology na igwe mmụta usoro, nke nyere ultra-elu nchọpụta sensitivity maka ụkwara nta na ole na ole nje bacteria na ụkwara nta meningitis, nyere a ọhụrụ hypersensitivity nke ụdị ọrịa tuber nchoputa nke abụọ ụzọ nchoputa nke nchoputa nke tuberculosis. nchọpụta na-eguzogide ọgwụ na ọgwụgwọ ụkwara nta. N'otu oge ahụ, a chọpụtara na plasma cfDNA nke onye ọrịa nwere ike iji dị ka ụdị nlele kwesịrị ekwesị maka nlele ụlọ ọgwụ na nyocha nke TBM.
N'ime ọmụmụ ihe a, a na-eji 227 plasma sample na cerebrospinal fluid sample iji guzobe ndị otu ụlọ ọgwụ abụọ, bụ nke a na-eji nyocha nyocha ụlọ nyocha iji guzobe usoro mmụta igwe nke nchoputa ụkwara nta, na-eji nyocha nyocha nke ụlọ ọgwụ iji nyochaa ụdị nchọpụta nchọpụta e guzobere. Ebu ụzọ lekwasịrị anya nlele niile site na ọdọ mmiri nyocha echere echere maka ụkwara nta Mycobacterium. Mgbe ahụ, dabere na data usoro nke TB-tNGS, a na-eji ihe atụ osisi mkpebi mee nkwenye 5-fold cross-validation na ọzụzụ na nkwado nke ụlọ nyocha nyocha ụlọ nyocha, na-enwetakwa ọnụ ụzọ nyocha nke ihe nlele plasma na nlele mmiri cerebrospinal. A na-ewebata ọnụ ụzọ enwetara n'ime usoro nyocha abụọ nke kwụ n'ahịrị nyocha ụlọ ọgwụ maka nchọpụta, yana ROC curve na-enyocha arụmọrụ nke onye mmụta. N'ikpeazụ, e nwetara ụdị nchoputa nke ụkwara nta.
Fig. 1 schematic eserese nke ime nyocha
Nsonaazụ: Dị ka ọnụ ụzọ dị iche iche nke CSF DNA sample (AUC = 0.974) na plasma cfDNA sample (AUC = 0.908) kpebisiri ike na ọmụmụ a, n'etiti 227 samples, uche nke CSF sample bụ 97.01%, kpọmkwem bụ 95.65%, na mmetụta uche 1% na plasma bụ 8.3%. Na nyocha nke 44 paired samples nke plasma cfDNA na cerebrospinal fluid DNA sitere na ndị ọrịa TBM, usoro nyocha nke ọmụmụ a nwere oke ngbanwe nke 90.91% (40/44) na plasma cfDNA na cerebrospinal fluid DNA, na uche bụ 95.45% (42/44). N'ime ụmụaka nwere ụkwara nta nke akpa ume, usoro nyocha nke ọmụmụ a na-enwe mmetụta nke ọma na ihe nlele plasma karịa ihe nchọpụta Xpert nke ihe ọṅụṅụ gastric si n'otu ndị ọrịa (28.57% VS 15.38%).
Fig. 2 Ọrụ nyocha nke ụdị nyocha ụkwara nta maka nlele ọnụ ọgụgụ mmadụ
Fig. 3 Nsonaazụ nchọpụta nke ihe atụ jikọtara ọnụ
Mkpebi: E guzobere usoro nchọpụta hypersensitive maka ụkwara nta n'ime ọmụmụ ihe a, nke nwere ike inye ngwá ọrụ nyocha nke nwere mmetụta nchọpụta kachasị elu maka ndị ọrịa na-agwọ ọrịa na-arịa ọrịa oligobacillary tuberculosis (omenala na-adịghị mma). Nchọpụta ụkwara nta hypersensitive dabere na plasma cfDNA nwere ike ịbụ ụdị nlele kwesịrị ekwesị maka nchọpụta ụkwara nta na-arụ ọrụ na ụkwara nta maningitis (ntụpọ plasma dị mfe ịnakọta karịa mmiri cerebrospinal maka ndị ọrịa a na-enyo enyo na ọ bụ ụkwara nta ụbụrụ).
Njikọ mbụ: https://www.sciencedirect.com/science/article/pii/s0009898123004990? site na%3Dihub
Mwebata dị nkenke nke ngwaahịa nchọpụta usoro ụkwara nta nke Macro & Micro-Test
N'iburu n'uche ọnọdụ nlele mgbagwoju anya nke ndị ọrịa ụkwara nta na mkpa dị iche iche, Macro & Micro-Test na-enye ngwọta NGS zuru ezu maka mmịpụta liquefaction site na sputum sample, ụlọ akwụkwọ Qualcomm na nhazi usoro, na nyocha data. Ngwaahịa a na-ekpuchi nyocha ngwa ngwa nke ndị ọrịa ụkwara nta, nchọpụta ọgwụ na-eguzogide ụkwara nta, ụdị ụkwara nta mycobacterium na NTM, nchọpụta hypersensitivity nke ọrịa ụkwara nta na-adịghị mma na ndị nwere ụkwara nta, wdg.
Ngwa nchọpụta usoro maka ụkwara nta na mycobacteria:
| Ihe Mba | aha ngwaahịa | ọdịnaya ule ngwaahịa | ụdị sample | ihe nlereanya |
| HWTS-3012 | Ihe ntọhapụ sample | Ejiri ya na ọgwụgwọ liquefaction nke sputum sample, enwetala nọmba ndekọ nke mbụ, Sutong Machinery Equipment 20230047. | sputum | |
| HWTS-NGS-P00021 | Ngwa nchọpụta ọnụọgụ nke Qualcomm maka ụkwara nta nwere mmetụta (usoro nyocha) | Nchọpụta nke na-adịghị emerụ ahụ (mmiri mmiri mmiri) nchọpụta hypersensitivity maka bacteria-adịghị mma ụkwara nta pulmonary na ụbụrụ nodules; A na-enyocha ihe nlele nke ndị a na-enyo enyo na ha nwere ụkwara nta ma ọ bụ ndị na-abụghị ụkwara nta mycobacteria site na usoro metagenomics dị elu dị elu, na ozi nchọpụta nke ma ụkwara nta ma ọ bụ na-abụghị ụkwara nta mycobacteria ebutere ya na isi ihe mbụ na-eguzogide ọgwụ na-eguzogide ọgwụ nke mycobacterium ụkwara nta. | Ọbara dị n'akụkụ, mmiri mmiri lavage alveolar, hydrothorax na ascites, ihe nlele nleba anya, mmiri cerebrospinal. | Ọgbọ nke abụọ |
| HWTS-NGS-T001 | Mpịnye mycobacterium na ngwa nchọpụta nguzogide ọgwụ (usoro nkwuwapụta ọtụtụ) | Nnwale pịnye mycobacterium, gụnyere MTBC na 187 NTM;Nchọpụta nguzogide ọgwụ ọjọọ nke ụkwara nta Mycobacterium na-ekpuchi ọgwụ iri na atọ na saịtị 16 bụ isi nke mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ. | Sputum, mmiri mmiri lavage alveolar, hydrothorax na ascites, ihe nleba anya nhụsianya, mmiri mmiri cerebrospinal. | Nke abụọ / atọ ọgbọ abụọ ikpo okwu |
Isi ihe: HWTS-NGS-T001 Mycobacterium pịnye na ngwa nchọpụta na-eguzogide ọgwụ (usoro mmụba multiplex)
Ntinye ngwaahịa
Ngwaahịa a dabere na isi ọgwụ nke mbụ na nke abụọ akọwapụtara na ntuziaka ọgwụgwọ TB nke WHO, macrolides na aminoglycosides nke a na-ejikarị na ntuziaka ọgwụgwọ NTM, saịtị ndị na-eguzogide ọgwụ na-ekpuchi otu saịtị metụtara ọgwụ mgbochi ọgwụ na WHO Mycobacterium tuberculosis complex mutation katalọgụ, yana ndị ọzọ akọwara mkpụrụ ndụ ọgwụ na-eguzogide ọgwụ na saịtị mmụgharị dị ka nyocha nke ụlọ na scostat dị na mba ofesi.
Ngosipụta akara sitere na ụdị NTM nke achịkọtara na ntuziaka NTM nke Chinese Journal of Tuberculosis and Respiratory Diseases bipụtara na nkwenye nke ndị ọkachamara. Mpempe mpempe akwụkwọ ndị a haziri nwere ike ịbawanye, usoro na nkọwapụta karịa ụdị 190 NTM.
Site na teknụzụ mmụba PCR multiplex ezubere iche, mkpụrụ ndụ ihe nketa genotyping na mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ nke Mycobacterium bụ PCR multiplex na-abawanye, na ngwakọta amplicon nke mkpụrụ ndụ ihe nketa a ga-achọpụta. Enwere ike iwulite ngwaahịa ndị a gbasasịrị n'ọbá akwụkwọ nke ọgbọ nke abụọ ma ọ bụ ọgbọ nke atọ, yana usoro nhazi usoro ọgbọ nke abụọ na ọgbọ nke atọ nwere ike idobe usoro usoro omimi dị elu iji nweta ozi usoro nke mkpụrụ ndụ ihe nketa. Site n'iji atụnyere ngbanwe ndị a ma ama dị na nchekwa data arụnyere arụnyere (gụnyere WHO Mycobacterium tuberculosis complex mutation katalọgụ na mmekọrịta ya na nguzogide ọgwụ), achọpụtara ngbanwe ndị metụtara nguzogide ọgwụ ma ọ bụ ike nke ọgwụ mgbochi ụkwara nta. Ejikọtara ya na ngwọta ọgwụgwọ sputum nke mepere emepe nke Macro & Micro-Test, nsogbu nke obere nucleic acid amplification arụmọrụ nke nlele sputum ụlọ ọgwụ (ugboro iri dị elu karịa nke usoro ọdịnala) edoziziri, nke mere na a ga-etinye nrịanrịa nkwụsi ike ọgwụ ozugbo na nlele sputum ụlọ ọgwụ.
Oke nchọpụta ngwaahịa
34mkpụrụ ndụ ihe nketa metụtara ọgwụ18ọgwụ mgbochi ụkwara nta na6Achọpụtara ọgwụ NTM, na-ekpuchi297Ebe ndị na-eguzogide ọgwụ; Ụdị ụkwara nta nke mycobacterium iri na karịa190Achọpụtara ụdị NTM dị iche iche.
Tebụl 1: Ozi nke ọgwụ 18+6 +190+NTM
Uru ngwaahịa
Mgbanwe ụlọ ọgwụ siri ike: enwere ike ịchọpụta ihe nlele sputum ozugbo site na onye na-aṅụ mmanya onwe ya na-enweghị omenala.
Ọrụ nnwale ahụ dị mfe: agba nke mbụ nke ime nkwalite dị mfe, a na-ewu ụlọ ọba akwụkwọ n'ime awa 3, nke na-eme ka arụmọrụ ọrụ dịkwuo mma.
Mpempe akwụkwọ zuru oke na nguzogide ọgwụ: na-ekpuchi saịtị ntinye na ọgwụ na-eguzogide ọgwụ nke MTB na NTM, nke bụ isi ihe na-echegbu ụlọ ọgwụ, ntinye ziri ezi na nchọpụta nkwụsị ọgwụ, na-akwado ngwanrọ nyocha onwe onye, na ịmepụta akụkọ nyocha na otu ọpịpị.
Ndakọrịta: ndakọrịta ngwaahịa, ime mgbanwe na nyiwe ILM na MGI/ONT.
Nkọwapụta ngwaahịa
| Koodu ngwaahịa | aha ngwaahịa | ikpo okwu nchọpụta | nkọwa |
| HWTS-NGS-T001 | Mpempe akwụkwọ mycobacterium na ngwa nchọpụta nguzogide ọgwụ (usoro mmụba multiplex) | ONT, Illumina, MGI, Salus pro | 16/96rxn |
Oge nzipu: Jan-23-2024