Mgbanwe ntụnyere na mkpụrụ ndụ ihe nketa KRAS na-etinye aka n'ọtụtụ etuto mmadụ, yana ngbanwe dị ihe dịka 17%-25% n'ofe ụdị etuto ahụ, 15%-30% na ọrịa kansa akpa ume, yana 20%-50% n'ọrịa cancer colorectal. Mgbanwe ndị a na-akwalite nguzogide ọgwụgwọ na ọganihu etuto site na usoro isi: protein P21 nke KRAS na-arụ ọrụ na ala nke ụzọ akara EGFR. Ozugbo a gbanwere KRAS, ọ na-eme ka akara ngosi mgbada na-arụ ọrụ mgbe niile, na-eme ka usoro ọgwụgwọ EGFR gbagoro elu ghara ịdị irè ma na-eduga n'ịbawanye mkpụrụ ndụ ọjọọ. N'ihi ya, mmụgharị KRAS jikọtara ya na iguzogide EGFR tyrosine kinase inhibitors na ọrịa cancer akpa ume yana na ọgwụgwọ mgbochi EGFR na ọrịa cancer colorectal.

Na 2008, National Comprehensive Cancer Network (NCCN) hiwere ụkpụrụ ụlọ ọgwụ na-akwado nnwale mgbanwe KRAS maka ndị ọrịa niile nwere ọrịa kansa metastatic colorectal (mCRC) tupu ọgwụgwọ. Ntuziaka a na-eme ka ọ pụta ìhè na ihe ka ọtụtụ n'ime mgbanwe KRAS na-arụ ọrụ na-eme na codons 12 na 13 nke exon 2. Ya mere, nchọpụta mmụgharị KRAS ngwa ngwa na nke ziri ezi dị mkpa maka iduzi ọgwụgwọ ahụike kwesịrị ekwesị.

Ihe kpatara Nnwale KRAS ji dị mkpa naMetastaticCelerectalCancer(mCRC)

Ọrịa cancer agba (CRC) abụghị otu ọrịa kama nchịkọta nke ụdị subtypes dị iche iche. Mgbanwe nke KRAS-nke dị na ihe dịka 40-45% nke ndị ọrịa CRC-na-eme dị ka mgbanwe "na" mgbe niile, na-akwalite uto cancer na-adabere na mgbama mpụga. Maka ndị ọrịa nwere mCRC, ọkwa KRAS na-ekpebi ịdị irè nke mgbochi EGFR monoclonal antibodies dị ka Cetuximab na Panitumumab:

Ụdị anụ ọhịa KRAS:O yikarịrị ka ndị ọrịa ga-erite uru na ọgwụgwọ mgbochi EGFR.

Mgbanwe KRAS:Ndị ọrịa na-enwetaghị uru ọ bụla site na ndị ọrụ ndị a, na-etinye aka na mmetụta ndị na-adịghị mkpa, mmụba na-akwụ ụgwọ, na igbu oge na ọgwụgwọ dị irè.

Nnwale KRAS ziri ezi na nke nwere mmetụta bụ isi nkuku nke atụmatụ ọgwụgwọ ahaziri onwe ya.

Ihe ịma aka achọpụta: ikewapụ akara ngbanwe

Ụzọ ọdịnala na-enwekarị mmetụta maka mmụgharị dị ala, ọkachasị n'omume nwere ọdịnaya etuto dị ala ma ọ bụ mgbe emechara ya. Ihe isi ike dị n'ịmata ọdịiche dị n'ụdị DNA nke na-adịghị ike megide ụdị ụdị anụ ọhịa dị elu-dị ka ịchọta agịga n'ime ahịhịa. Nsonaazụ na-ezighi ezi nwere ike ibute ọgwụgwọ ezighi ezi yana nsonaazụ mebie.

Ngwọta anyị: Emepụtara nkenke maka nchọpụta mmụgharị obi siri ike

Ngwa nchọpụta Mgbanwe KRAS anyị na-ejikọta teknụzụ dị elu iji merie njedebe ndị a, na-ebuga izi ezi na ntụkwasị obi maka nduzi ọgwụgwọ mCRC.

Kedu ka Teknụzụ Anyị si agba mbọ hụ na arụmọrụ kacha mma

• Teknụzụ ARMS emelitere (Sistemụ Mgbanwe Refractory Amplification): Na-ewulite n'elu teknụzụ ARMS, na-etinye teknụzụ nkwalite nke nwe ya iji welie nchọta nkọwapụta.
• Enzymatic Enrichment: Na-eji mmachi endonucleases gbarie ihe ka ọtụtụ n'ime ụdị anụ ọhịa genome nke mmadụ, na-echekwa ụdị mutant, si otú a na-eme ka nchọpụta nchọpụta dịkwuo elu ma na-ebelata mmụba na-abụghị nke a kapịrị ọnụ n'ihi nzụlite genomic dị elu.
• Mgbochi okpomoku: Na-ewebata usoro okpomọkụ akọwapụtara na usoro PCR, na-eme ka ndakọrịta dị n'etiti primers mutant na ụdị anụ ọhịa, si otú a na-ebelata ndabere ụdị anụ ọhịa na imezi mkpebi nchọpụta.
• Mmetụta dị elu: Na-achọpụta nke ọma ka DNA mutant 1% dị ala.
• Ezigbo mma: Na-eji ụkpụrụ ime yana enzyme UNG iji gbochie nsonaazụ ụgha na nke na-adịghị mma.
• Dị Mfe na Ọsọ: Na-emecha nnwale n'ime ihe dị ka nkeji 120, na-eji tubes mmeghachi omume abụọ iji kwado nchọpụta mmụkọ asatọ dị iche iche, na-arụpụta ebumnuche na nsonaazụ ntụkwasị obi.
• Ngwa ndakọrịta: Na-adaba na ngwa PCR dị iche iche.

Ọgwụ nke ọma na ọrịa kansa colorectal na-amalite site na nyocha nke mkpụrụ ndụ. Site na ịnakwere ngwa nchọpụta Mgbanwe KRAS anyị, ụlọ nyocha gị nwere ike wepụta nsonaazụ ga-arụ ọrụ nke na-akpụzi ụzọ ọgwụgwọ onye ọrịa ozugbo.

Kwado ụlọ nyocha gị site na teknụzụ a pụrụ ịdabere na ya - ma mee ka nlekọta ahaziri onwe ya n'ezie.

Kpọtụrụ anyị: marketing@mmtest.Com

Mụtakwuo maka ijikọ ihe ngwọta a dị elu n'ime usoro ọrụ nyocha gị.

#Colorectal #Cancer #DNA #Mutation #Precision #Ezubere # Ọgwụgwọ #Cancer

Imeghe ọgwụ ziri ezi na ọrịa kansa colorectal

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