Ụdị itoolu nke nje virus na-eku ume
Aha ngwaahịa
HWTS-RT185A-ụdị itoolu nke ngwa iku ume Nucleic Acid (Fluorescence PCR)
Epidemiology
Ọrịa iku ume bụ ụdị ọrịa a na-ahụkarị nke mmadụ, nke nwere ike ime na okike, afọ na mpaghara ọ bụla, ma bụrụ otu n'ime ihe kacha akpata ọrịa na ọnwụ n'ụwa.[1]. Ọrịa iku ume na-ahụkarị na-agụnye nje influenza A (IFV A), nje influenza B (IFV B), coronavirus novel (SARS-CoV-2), nje syncytial respiratory, adenovirus, metapneumovirus mmadụ, rhinovirus, nje parainfluenza (I/II/III) na mycoplasma pneumoniae, wdg.[2,3]. Mgbaàmà nke ụlọ ọgwụ na ihe ịrịba ama nke ọrịa iku ume na-akpata yiri nke ahụ, ma ọrịa nke ọrịa dị iche iche na-akpata nwere usoro ọgwụgwọ dị iche iche, mmetụta ọgwụgwọ na usoro ọrịa.[4,5]. Ka ọ dị ugbu a, ụzọ bụ isi nke nchọpụta ụlọ nyocha nke ọrịa iku ume gụnyere: iche iche nje, nchọpụta antigen na nchọpụta nucleic acid. Ngwa a na-achọpụta ma na-achọpụta ụfọdụ acid nucleic viral kpọmkwem n'ime ndị mmadụ nwere akara na mgbaàmà nke ọrịa iku ume, yana njikọ ụlọ ọgwụ ndị ọzọ na ụlọ nyocha iji nyere aka na nyocha nke ọrịa nje iku ume.
Ọwa
| FAM | MP nucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | 2-8 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 mbipụta/ml |
| Nkọwapụta | Cross reactivity: Ọ dịghị cross reactivity n'etiti kit na Boca nje, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, mmadụ coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, pneptococcusiella, Streptococcusiella. ka oyi baa, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium ụkwara nta, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptoansssalicoccus nephrine catarrh, Lactobacillus, Corynebacterium, DNA genomic mmadụ. Nnwale nnyonye anya: họrọ mucin (60mg / ml), ọbara mmadụ (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL, sodium chloride na 5% preservative (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mtone) (2mg/ml), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupiromcin (1mg/mL), mupiromcin (20mg/mL), oseltamivir (60ng / mL), ribavirin (10mg / L), Nsonaazụ gosiri na ihe ndị na-etinye aka na ntinye uche dị n'elu enweghị mmeghachi omume na-egbochi nchoputa nke ngwa ahụ. |
| Ngwa ndị ọdabara | Ọdabara na ụdị m nchọpụta reagent: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler), Co.RzhouTime Mo.9 Sistemụ PCR, BioRad CFX Opus 96 Sistemụ PCR ezigbo oge. Ọdabara na ụdị II reagent nchọpụta: EudemonTM AIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
| Nchekwa | 2-8 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 mbipụta/ml |
| Nkọwapụta | Cross reactivity: Ọ dịghị cross reactivity n'etiti kit na Boca nje, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, mmadụ coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, pneptococcusiella, Streptococcusiella. ka oyi baa, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium ụkwara nta, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptoansssalicoccus nephrine catarrh, Lactobacillus, Corynebacterium, DNA genomic mmadụ. Nnwale nnyonye anya: họrọ mucin (60mg / ml), ọbara mmadụ (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL, sodium chloride na 5% preservative (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mtone) (2mg/ml), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupiromcin (1mg/mL), mupiromcin (20mg/mL), oseltamivir (60ng / mL), ribavirin (10mg / L), Nsonaazụ gosiri na ihe ndị na-etinye aka na ntinye uche dị n'elu enweghị mmeghachi omume na-egbochi nchoputa nke ngwa ahụ. |
| Ngwa ndị ọdabara | Ọdabara na ụdị m nchọpụta reagent: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler), Co.RzhouTime Mo.9 Sistemụ PCR, BioRad CFX Opus 96 Sistemụ PCR ezigbo oge. Ọdabara na ụdị II reagent nchọpụta: EudemonTM AIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro ọrụ
Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-8 nwere ike iji E).TM AIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ihe nlele nke ewepụtara bụ 200μL yana olu elution akwadoro bụ 150μL.
