Otu B Streptococcus Nucleic Acid
Aha ngwaahịa
HWTS-UR010A-Nucleic Acid Nchọpụta Ngwa dabere na Enzymatic Probe Isothermal Amplification (EPIA) maka Group B Streptococcus
Epidemiology
Group B Streptococcus (GBS), nke a makwaara dị ka streptococcus agalcatiae, bụ nje na-akpata gram-positive nke na-ebikarị na tract digestive dị ala na urogenital nke ahụ mmadụ. Ihe dị ka 10%-30% nke ụmụ nwanyị dị ime nwere ebe obibi GBS. Ụmụ nwanyị dị ime na-enwe ike ịnweta GBS n'ihi mgbanwe na gburugburu ebe obibi nke usoro ọmụmụ nke mgbanwe na ọkwa hormone dị na ahụ, nke nwere ike ịkpata nsogbu ime ime dị ka ịmụ nwa, mgbawa nke akpụkpọ ahụ, na ịmụ nwa, ma nwekwara ike ibute ọrịa puerperal n'ime ụmụ nwanyị dị ime. Na mgbakwunye, 40%-70% nke ndị inyom bu ọrịa GBS ga-ebufe GBS nye ụmụ amụrụ ọhụrụ ha n'oge a na-amụ nwa site na oghere ọmụmụ, na-ebute ọrịa na-efe efe nke nwa ọhụrụ dị ka sepsis ọhụrụ na meningitis. Ọ bụrụ na ụmụ amụrụ ọhụrụ na-ebu GBS, ihe dịka 1%-3% n'ime ha ga-ebute ọrịa na-efe efe n'oge, na 5% ga-eduga ọnwụ. Otu nwa amụrụ ọhụrụ B streptococcus jikọtara ya na ọrịa na-efe efe na-efe efe na ọ bụ ihe dị mkpa na-ebute ọrịa na-efe efe siri ike dị ka sepsis ọhụrụ na meningitis. Ngwa a na-achọpụta n'ụzọ ziri ezi ọrịa nke otu B streptococcus iji belata ọnụọgụ na mmerụ ahụ ya na ụmụ nwanyị dị ime na ụmụ amụrụ ọhụrụ yana ibu arọ akụ na ụba na-enweghị isi nke mmerụ ahụ kpatara.
Ọwa
| FAM | GBS nucleic acid |
| ROX | ntinye aka n'ime |
Nka nka
| Nchekwa | Mmiri: ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Genital tract na rectal secretions |
| Tt | .30 |
| CV | ≤10.0% |
| LoD | 500 oyiri/ml |
| Nkọwapụta | Ọ dịghị cross-reactivity na ndị ọzọ genital tract na rectal swab samples dị ka Candida albicans, Trichomonas vaginalis, Chlamydia trachomatis, Ureaplasma urealyticum, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus, Garacpidella virus, Humanalis pallomavirus. Staphylococcus aureus, ntụaka na-adịghị mma nke mba N1-N10 (Streptococcus pneumoniae, Pyogenic streptococcus, Streptococcus thermophilus, Streptococcus mutans, Streptococcus pyogenes, Lactobacillus acidophilus, Lactobacillus acidophilus, Lactobacillus reutericous, DH5scherichia, na Alfarobiccharic, DH5scheric, na Alfascheric. DNA nke genomic |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR SistemuNgwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®480 ezigbo oge PCR usoro Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
