Acid Nucleic nke Orientia
Aha ngwaahịa
HWTS-OT002-Orientia tsutsugamushi Ngwa nchọpụta Nucleic Acid(Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Scrub typhus bụ ọrịa ọkụ nke ọrịa Orientia tsutsugamushi (Ot) kpatara. Ọrịa Orientia scrub typhus bụ nje nje nke na-akpata nje bacteria dị n'ime sel nke na-akpata nje Gram-negative. Ọrịa Orientia scrub typhus bụ nke ụdị Orientia dịka Rickettsiales si dị, ezinụlọ Rickettsiaceae, na ụdị Orientia. A na-ebutekarị Scrub typhus site na nta nke ụmụ ahụhụ chigger na-ebu nje. A na-amata ya site na oke ọkụ mberede, eschar, lymphadenopathy, hepatosplenomegaly, na leukopenia ọbara dị n'akụkụ, wdg. N'ọnọdụ ndị siri ike, ọ nwere ike ibute meningitis, ọdịda imeju na akụrụ, ọdịda akụkụ ahụ dị iche iche, na ọbụna ọnwụ.
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | ihe nlele ọbara |
| CV | ≤5.0% |
| LoD | Mbipụta 500/mL |
| Ngwa Ndị Dị Mkpa | Ọ dabara na ihe nchọpụta ụdị I:Sistemụ PCR Oge-N'ezie nke Biosystems 7500, QuantStudio®Sistemụ PCR 5 n'oge a, Sistemụ PCR SLAN-96P n'oge a na-ahụ anya (Hongshi Medical Technology Co., Ltd.), Sistemụ Nchọpụta PCR LineGene 9600 Plus nke oge a (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.), Sistemụ PCR oge-oge BioRad CFX96, Sistemụ PCR BioRad CFX Opus 96 n'oge dị adị. Ọ dabara na ihe nchọpụta ụdị II: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro Ọrụ
Ngwa Ngwuputa DNA/RNA nke Macro & Micro-Test General (HWTS-3019) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. A ga-eme mwepụta ahụ dịka ntuziaka maka ojiji nke ihe mwepụta a si dị. Olu ihe nlele ewepụtara bụ 200μL, olu mwepụta akwadoro bụ 100μL.
