Orientia tsutsugamushi Nucleic acid
Aha ngwaahịa
HWTS-OT002-Orientia tsutsugamushi Ngwa nchọpụta Nucleic Acid(Fluorescence PCR)
Epidemiology
Scrub typhus bụ nnukwu ọrịa febrile nke Orientia tsutsugamushi (Ot) na-ebute. Orientia scrub typhus bụ microorganism parasitic intracellular gram-adịghị mma. Orientia scrub typhus bụ nke genus Orientia n'usoro Rickettsiales, ezinụlọ Rickettsiaceae, na genus Orientia. A na-ebutekarị typhus scrub site na ata nke chigger larvae na-ebu nje. Ọ na clinically e ji na mberede elu ahụ ọkụ, eschar, lymphadenopathy, hepatosplenomegaly, na mpụta ọbara leukopenia, wdg Na oké njọ ikpe, ọ pụrụ ime ka meningitis, imeju na akụrụ ọdịda, systemic multi-organ ọdịda, na ọbụna ọnwụ.
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | serum samples |
| CV | ≤5.0% |
| LoD | 500 oyiri/μL |
| Ngwa ndị ọdabara | Ọdabara na ụdị I nchọpụta reagent: Ngwa Biosystem 7500 ezigbo oge PCR, QuantStudio®Sistemụ PCR 5 ezigbo oge, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 PCR ezigbo oge. Ọdabara maka ụdị II reagent nchọpụta: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro ọrụ
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. A ga-eduzi mmịpụta dị ka ntuziaka maka iji nke a mmịpụta rea. Ihe nlele nke ewepụtara bụ 200μL, na olu elution akwadoro bụ 100μL.
