Orientia tsutsugamushi
Aha ngwaahịa
HWTS-OT002B Orientia tsutsugamushiNgwa Nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Scrub typhus bụ nnukwu ọrịa febrile nke Orientia tsutsugamushi (Ot) na-ebute. Orientia scrub typhus bụ microorganism parasitic intracellular gram-adịghị mma. Orientia scrub typhus bụ nke genus Orientia n'usoro Rickettsiales, ezinụlọ Rickettsiaceae, na genus Orientia. A na-ebutekarị typhus scrub site na ata nke chigger larvae na-ebu nje. Ọ na clinically e ji na mberede elu ahụ ọkụ, eschar, lymphadenopathy, hepatosplenomegaly, na mpụta ọbara leukopenia, wdg Na oké njọ ikpe, ọ pụrụ ime ka meningitis, imeju na akụrụ ọdịda, systemic multi-organ ọdịda, na ọbụna ọnwụ.
Ọwa
| FAM | Orientia tsutsugamushi |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | ọbara ọhụrụ |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 oyiri/μL |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 ezigbo oge PCR usoro Sistemụ Nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 ezigbo oge PCR Sistemu |
Usoro ọrụ
Reagenti mmịpụta akwadoro: Macro & Micro-TestIzugbeNgwa DNA/RNA (HWTS-3019) (nke nwere ike iji na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) site Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The mmịpụta ga-eduzi dị ka ntuziaka maka ojiji nke a mmịpụta reagent. Olu nlele ewepụtara bụ 200µL, na olu elution akwadoro bụ100µL.
