Orientia tsutsugamushi
Aha ngwaahịa
HWTS-OT002B Orientia tsutsugamushiNgwa Nchọpụta Asịd Nucleic (PCR Fluorescence)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Scrub typhus bụ ọrịa ọkụ nke ọrịa Orientia tsutsugamushi (Ot) kpatara. Ọrịa Orientia scrub typhus bụ nje nje nke na-akpata nje bacteria dị n'ime sel nke na-akpata nje Gram-negative. Ọrịa Orientia scrub typhus bụ nke ụdị Orientia dịka Rickettsiales si dị, ezinụlọ Rickettsiaceae, na ụdị Orientia. A na-ebutekarị Scrub typhus site na nta nke ụmụ ahụhụ chigger na-ebu nje. A na-amata ya site na oke ọkụ mberede, eschar, lymphadenopathy, hepatosplenomegaly, na leukopenia ọbara dị n'akụkụ, wdg. N'ọnọdụ ndị siri ike, ọ nwere ike ibute meningitis, ọdịda imeju na akụrụ, ọdịda akụkụ ahụ dị iche iche, na ọbụna ọnwụ.
Ọwa
| FAM | Orientia tsutsugamushi |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | serum ọhụrụ |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Mbipụta 500/μL |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR BioRad CFX96 n'oge nkịtị, Sistemụ PCR BioRad CFX Opus 96 n'oge nkịtị |
Usoro Ọrụ
Ihe na-eme ka a na-ewepụta ihe a tụrụ aro: Macro na Micro-TestIzugbeNgwa DNA/RNA (HWTS-301)9) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. A ga-eme mwepụta ahụ dịka ntuziaka maka ojiji nke ihe mwepụta a si dị. Olu ihe nlele ewepụtara bụ 200µL, olu mwepụta akwadoro bụ100µL.
