Ihe Ndị Na-akpata Ọrịa Iku Oku Ejikọtara

Nkọwa Dị Mkpirikpi:

A na-eji ngwa a achọpụta nje influenza A, nje influenza B, nje respiratory syncytial, adenovirus, nje rhinovirus mmadụ na mycoplasma pneumoniae nucleic acids n'ime swabs nasopharyngeal mmadụ na ihe nlele swab oropharyngeal. Enwere ike iji nsonaazụ nnwale ahụ nyere aka na nchọpụta nke nje na-efe efe, ma nye ntọala nchọpụta molekul maka nchọpụta na ọgwụgwọ nke nje na-efe efe.

Zitere anyị ozi-e

Nkọwa Ngwaahịa

Akara Ngwaahịa

Vidiyo ndị yiri ya

Nzaghachi (2)

Iji mezuo ihe ndị ahịa chọrọ nke ọma, a na-arụ ọrụ anyị niile nke ọma dịka ụkpụrụ anyị si dị, Ọkwa Asọmpi Dị Elu, Ọrụ Dị Ngwa Ngwa makaDenver-4, Ngwa Nchọpụta Vitamin D, Ọrịa Kansa nke KrasIji mụtakwuo maka ihe anyị nwere ike imere gị, kpọtụrụ anyị n'oge ọ bụla. Anyị na-atụ anya ịmalite mmekọrịta azụmaahịa dị mma na nke ogologo oge na gị.

Nkọwa nke Ọrịa Iku Oku:

Aha ngwaahịa

Ngwa Nchọpụta Acid Nucleic nke HWTS-RT050-Ụdị Isii nke Ọrịa Iku Oku(PCR fluorescence)

Ọmụmụ ihe gbasara ọrịa na-efe efe

Influenza, nke a na-akpọkarị 'influenza', bụ ọrịa na-efe efe nke nje influenza na-akpata, nke na-efe efe nke ukwuu ma a na-ebutekarị ya site na ụkwara na imi.

Nje virus nke na-akpata mmeghachi omume iku ume (RSV) bụ nje RNA, nke sitere na ezinụlọ paramyxoviridae.

Ọrịa nje HIV nke mmadụ (HAdV) bụ nje DNA nke nwere njikọ abụọ na-enweghị envelopu. Achọpụtala ma ọ dịkarịa ala ụdị mkpụrụ ndụ ihe nketa 90, nke enwere ike kewaa n'ime 7 subgenera AG.

Ọrịa rhinovirus mmadụ (HRV) bụ otu n'ime ezinụlọ Picornaviridae na ụdị Enterovirus.

Mycoplasma pneumoniae (MP) bụ nje nje nke na-akpata ọrịa nke dị n'etiti nje bacteria na nje virus n'ogo.

Ọwa

Ọwa	PCR-Mix A	PCR-Mix B
Ọwa FAM	IFV A	HAdV
Ọwa VIC/HEX	HRV	IFV B
Ọwa CY5	RSV	MP
Ọwa ROX	Njikwa Dị n'ime	Njikwa Dị n'ime

Usoro Nkà na ụzụ

Nchekwa	-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ihe atụ	Swab nke oropharyngeal
Ct	≤35
LoD	500Mpempe/mL
Nkọwapụta	1.Nnwale nnwale mmeghachi omume gosiri na enweghị mmeghachi omume mmegharị ahụ n'etiti kit ahụ na nje SARSr-CoV nke mmadụ, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, ụdị nje Parainfluenza 1, 2, na 3, Chlamydia pneumoniae, nje metapneumovirus nke mmadụ, Enterovirus A, B, C, D, nje Epstein-Barr, nje Measles, nje cytomegalovirus nke mmadụ, Rotavirus, Norovirus, nje Mumps, nje Varicella-zoster, Legionella, Bordetella pertussis, influenza nke Haemophilus, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans na acids genomic genomic nke mmadụ. 2.Ike igbochi ihe mgbochi: Mucin (60mg/mL), 10% (v/v) ọbara mmadụ, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (nwere ihe nchekwa) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir A họọrọ (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxacin (10μg/mL), na tobramycin (0.6mg/mL) maka ule nnyonye anya, nsonaazụ ya gosikwara na ihe ndị na-egbochi ndị mmadụ na njupụta dị n'elu enweghị mmeghachi omume nnyonye anya na nsonaazụ ule nke nje.
Ngwa Ndị Dị Mkpa	Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio^®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler^®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR BioRad CFX96 n'oge nkịtị, Sistemụ PCR BioRad CFX Opus 96 n'oge nkịtị

Ngwọta PCR zuru oke

Ụdị ihe nchọpụta ihe na-akpata iku ume isii (Ngwa nchọpụta ihe na-akpata iku ume)

Foto nkọwa ngwaahịa:

Nduzi Ngwaahịa Ndị Yiri Ya:

Maka ọnụ ahịa dị oke njọ, anyị kwenyere na ị ga-achọ ihe ọ bụla ga-aka anyị mma. Anyị nwere ike ikwu n'ụzọ doro anya na maka ụdị mma dị otú a, anyị bụ ndị kacha ala maka ọrịa iku ume, nke a ga-enye n'ụwa niile, dịka: Sevilla, Yuropu, Mauritius. Ọ bụrụ na ị nwere mmasị na ngwaahịa anyị ọ bụla mgbe ị lere ndepụta ngwaahịa anyị anya, biko kpọtụrụ anyị maka ajụjụ. Ị ga-enwe ike izipu anyị ozi-e ma kpọtụrụ anyị maka ndụmọdụ, anyị ga-azakwa gị ozugbo anyị nwere ike. Ọ bụrụ na ọ dị mma, ị nwere ike ịchọta adreesị anyị na weebụsaịtị anyị wee bịa na ụlọ ọrụ anyị ma ọ bụ ozi ndị ọzọ gbasara ngwaahịa anyị n'onwe gị. Anyị dị njikere iwulite mmekọrịta ogologo na nke na-adịgide adịgide na ndị ahịa ọ bụla nwere ike ịzụta ihe n'ọhịa ndị metụtara ya.

zụta ngwa nchọpụta nje iku ume