Ejikọtara Ọrịa iku ume
Aha ngwaahịa
HWTS-RT050-ụdị isii nke ihe nchọpụta ihe nrịba ama n'ime ume.(Pluorescence PCR)
Epidemiology
Influenza, nke a na-akpọkarị 'flu', bụ nnukwu ọrịa na-efe efe nke iku ume na-ebute site na nje influenza, nke na-efe efe nke ukwuu ma na-ebute ya site na ụkwara na imi.
Nje virus syncytial iku ume (RSV) bụ nje RNA, nke sitere na ezinụlọ paramyxoviridae.
Adenovirus mmadụ (HAdV) bụ nje DNA nwere eriri abụọ na-enweghị envelopu.Opekempe, achọtala genotypes 90, nke enwere ike kewaa na 7 subgenera AG.
Human rhinovirus (HRV) bụ otu n'ime ezinụlọ Picornaviridae na genus Enterovirus.
Mycoplasma pneumoniae (MP) bụ microorganism na-akpata ọrịa nke dị n'etiti nje bacteria na nje.
Ọwa
| Ọwa | PCR-Mix A | PCR-Mix B |
| Ọwa FAM | IFV A | HADV |
| Ọwa VIC/HEX | HRV | IFV B |
| Ọwa CY5 | RSV | MP |
| Ọwa ROX | Njikwa ime | Njikwa ime |
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Oropharyngeal swab |
| Ct | ≤35 |
| LoD | 500 oyiri/ml |
| Nkọwapụta | 1.Nsonaazụ nzaghachi mmeghachi omume gosipụtara na enweghị mmeghachi omume n'etiti ngwa ahụ na coronavirus mmadụ SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, Parainfluenza nje ụdị 1, 2, na 3, Chlamydia pneumoniae, metapneumovirus mmadụ, Enterovirus A, B, C, D, nje Epstein-Barr, nje Measles, mmadụ cytomegalovirus, Rotavirus, Norovirus, Mumps virus, Varicella-zoster virus, Legionella, Bordetella pertussis, Haemophilus influencozae. aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium ụkwara nta, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans na mmadụ genomic acid nucleic. 2.Ikike mgbochi nnyonye anya: Mucin (60mg / ml), 10% (v / v) ọbara mmadụ, phenylephrine (2mg / mL), oxymetazoline (2mg / mL), sodium chloride (ya na ihe nchekwa) (20mg / mL), beclomethasone ( 20mg / ml), dexamethasone (20mg / ml), flunisolide (20μg / ml), triamcinolone acetonide (2mg / ml), budesonide (2mg / ml), mometasone (2mg / ml), Fluticasone (2mg / ml), histamine hydrochloride. (5mg/mL), alpha-interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), Ritonavir (60mg / ml), mupirocin (20mg / ml), azithromycin (1mg / ml), cefprozil (40μg / ml), Meropenem (200mg / ml), levofloxacin (10μg / ml), na tobramycin (0.6mg/mL). ahọpụtara maka nyocha nnyonye anya, nsonaazụ ya gosipụtara na ihe ndị na-etinye aka na ntinye aka n'elu enweghị mmeghachi omume nnyonye anya na nsonaazụ ule nke nje. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 ezigbo oge PCR Sistemu |
Ngwọta PCR zuru oke
