Ụdị isii nke ọrịa iku ume

Ụdị nje nje iku ume isii egosipụtara onyogho

Nkọwa dị mkpirikpi:

Enwere ike iji ngwa a chọpụta nucleic acid nke SARS-CoV-2, nje influenza A, nje influenza B, adenovirus, mycoplasma pneumoniae na nje syncytial respiratory in vitro.

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HWTS-OT058A/B/C/Z-Ezigbo fluorescent RT-PCR kit maka ịchọpụta ụdị ọrịa iku ume isii.

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Epidemiology

Ọrịa Corona Virus 2019, nke akpọrọ "COVID-19", na-ezo aka na oyi baa nke ọrịa SARS-CoV-2 kpatara.SARS-CoV-2 bụ coronavirus nke β genus.COVID-19 bụ ọrịa na-efe efe na-efe efe na-efe efe na-efe efe, ndị mmadụ na-enwekarịkwa ike.Ka ọ dị ugbu a, isi ihe butere ọrịa bụ ndị ọrịa SARS-CoV-2 butere, yana ndị butere ọrịa asymptomatic nwekwara ike bụrụ isi iyi butere ọrịa.Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7.Ahụ ọkụ, ụkwara akọrọ na ike ọgwụgwụ bụ isi ihe ngosi.Ndị ọrịa ole na ole nwere mkpọchi imi, imi na-agba agba, akpịrị akpịrị, myalgia na afọ ọsịsa.

Influenza, nke a na-akpọkarị "flu", bụ nnukwu ọrịa na-efe efe nke iku ume nke nje influenza kpatara.Ọ na-efe efe nke ukwuu.A na-ebutekarị ya site na ụkwara na imi.Ọ na-apụtakarị na mmiri na oyi.A na-ekewa nje virus influenza n'ime influenza A, IFV A, influenza B, IFV B, na influenza C, IFV C ụdị atọ, ha niile bụ nje virus na-egbuke egbuke, na-ebute ọrịa mmadụ karịsịa maka nje nje A na B, ọ bụ otu eriri. segmented RNA nje.Influenza A bụ nnukwu ọrịa iku ume, gụnyere H1N1, H3N2 na ụdị ndị ọzọ, nke na-enwekarị mmụgharị na ntiwapụ n'ụwa nile."Shift" na-ezo aka na ngbanwe nke nje influenza A, na-ebute mpụta nke nje ọhụrụ "subtype".A na-ekewa nje virus influenza B ụzọ abụọ, Yamagata na Victoria.Nje virus influenza B nwere naanị ihe mgbochi antigenic, ọ na-ezere nleba anya na mkpochapụ nke sistemu ahụ ji alụso ọrịa ọgụ site na ngbanwe ya.Otú ọ dị, evolushọn ọsọ nke nje influenza B dị nwayọọ karịa nke nje influenza A mmadụ.Influenza B nwekwara ike bute ọrịa iku ume mmadụ ma bute ọrịa na-efe efe.

Adenovirus (AdV) bụ nke mammalian adenovirus, nke bụ nje DNA nwere eriri abụọ na-enweghị envelopu.Opekempe, a chọtara genotypes 90, nke enwere ike kewaa n'ime AG 7 subgenera.Ọrịa AdV nwere ike ibute ọrịa dị iche iche, gụnyere oyi baa, bronchitis, cystitis, conjunctivitis anya, ọrịa eriri afọ na encephalitis.Adenovirus oyi baa bụ otu n'ime ụdị oyi oyi ndị obodo na-enweta n'ime ụmụaka, na-aza ihe dịka 4%-10% nke oyi oyi ndị obodo nwetara.

Mycoplasma pneumoniae (MP) bụ ụdị obere prokaryotic microorganism, nke dị n'etiti nje bacteria na nje, nwere nhazi cell ma enweghị mgbidi cell.MP na-ebutekarị ọrịa iku ume mmadụ, ọkachasị ụmụaka na ndị ntorobịa.Ọ nwere ike ịkpata mycoplasma oyi baa mmadụ, ọrịa akụkụ iku ume ụmụaka na oyi baa nkịtị.Ihe ngosi ụlọ ọgwụ dị iche iche, ọtụtụ n'ime ha bụ ụkwara siri ike, ahụ ọkụ, oyi, isi ọwụwa, akpịrị akpịrị.Ọrịa elu akụkụ iku ume na oyi baa na bronchial bụ ihe a na-ahụkarị.Ụfọdụ ndị ọrịa nwere ike ịmalite site na ọrịa akụkụ iku ume elu ruo na oyi baa siri ike, nnukwu nsogbu iku ume na ọnwụ nwere ike ime.

Nje virus syncytial iku ume (RSV) bụ nje RNA, nke sitere na ezinụlọ paramyxoviridae.A na-ebute ya site na ụmụ irighiri mmiri ikuku na nso nso a na ọ bụ isi ihe na-akpata ọrịa iku ume n'ime ụmụ ọhụrụ.Ụmụaka nwere RSV nwere ike ịmalite bronchiolitis siri ike (nke a na-akpọ bronchiolitis) na oyi baa, bụ nke metụtara ụkwara ume ọkụ na ụmụaka.Ụmụaka na-enwe mgbaàmà siri ike, gụnyere nnukwu ahụ ọkụ, rhinitis, pharyngitis na laryngitis, na mgbe ahụ bronchiolitis na oyi baa.Ole na ole ụmụaka na-arịa ọrịa nwere ike mgbagwoju anya na otitis media, pleurisy na myocarditis, wdg. elu akụkụ okuku ume na tract ọrịa bụ isi ihe mgbaàmà nke ọrịa na ndị okenye na ndị okenye ụmụaka.

Ọwa

Aha nke ọwa	Ihe nchekwa mmeghachi omume R6 A	Ihe nchekwa mmeghachi omume R6 B
FAM	SARS-CoV-2	HADV
VIC/HEX	Njikwa ime	Njikwa ime
CY5	IFV A	MP
ROX	IFV B	RSV

Nka nka

Nchekwa	Mmiri: ≤-18℃ N'ọchịchịrị;Lyophilized: ≤30℃ N'ọchịchịrị
Ndụ nchekwa	Mmiri mmiri: ọnwa 9;Lyophilized: ọnwa 12
Ụdị ụdị	Ọbara zuru oke, Plasma, Serum
Ct	≤38
CV	≤5.0％
LoD	300 oyiri/ml
Nkọwapụta	Nsonaazụ mmeghachi omume gosipụtara na enweghị mmeghachi omume n'etiti ngwa ahụ na coronavirus mmadụ SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, nje parainfluenza ụdị 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus mmadụ, enterovirus A, B, C, D, virus pulmonary mmadụ, nje epstein-barr, nje measles, nje cytomegalo mmadụ, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium ụkwara nta, anwụrụ ọkụ aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci na amụrụ ọhụrụ cryptococcus na mmadụ genomic nucleic acid.
Ngwa ndị ọdabara	Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịaSistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR ABI 7500 ezigbo oge ABI 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio^®Sistemụ PCR 5 ezigbo oge LightCycler^®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 PCR ezigbo oge