Ureaplasma Parvum Nucleic Acid
Aha ngwaahịa
HWTS-UR046-Ureaplasma Parvum Nucleic Acid Nchọpụta Ngwa (Fluorescence PCR)
Epidemiology
A na-ekewa ụdị ureaplasma ugbu a na pathogenesis mmadụ n'ime biogroups 2 na serotypes 14. Biogroup Ⅰ bụ Ureaplasma urealyticum, nke na-agụnye serotypes: 2, 4, 5, 7, 8, 9, 10, 11, 12, na 13. Biogroup Ⅱ bụ Ureaplasma parvum, nke na-agụnye serotypes: 1, 3, 6, parasites a na-ahụkarị na parasites nwanyị bụ 14. usoro ọmụmụ na otu n'ime ihe ndị dị mkpa na-akpata ọrịa na-efe efe na usoro genitourinary. Na mgbakwunye na ịkpata ọrịa genitourinary, ụmụ nwanyị nwere ọrịa Ureaplasma nwekwara ike ibunye ndị ha na ha na-enwe mmekọahụ. Ọrịa Ureaplasma bụkwa otu n'ime ihe ndị dị mkpa na-akpata enweghị ọmụmụ. Ọ bụrụ na ụmụ nwanyị dị ime na-ebute Ureaplasma, ọ nwekwara ike iduga mgbawa nke membranes akaghi aka, nnyefe akaghi aka, ọrịa nhụsianya iku ume ọhụrụ, ọrịa ịmụ nwa na nsonaazụ ndị ọzọ dị njọ nke afọ ime, nke chọrọ nlebara anya dị ukwuu.
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | akụkụ mmamiri nwoke, akụkụ ọmụmụ nwanyị |
| Ct | ≤38 |
| CV | 5.0% |
| LoD | 400 oyiri/ml |
| Ngwa ndị ọdabara | Ọdabara na ụdị I nchọpụta reagent: Ngwa Biosystem 7500 ezigbo oge PCR, QuantStudio®Sistemụ PCR 5 ezigbo oge, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 PCR ezigbo oge. Ọdabara maka ụdị II reagent nchọpụta: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro ọrụ
Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-8 nwere ike iji E).TM AIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ihe nlele nke ewepụtara bụ 200μL yana olu elution akwadoro bụ 150μL.
