Ụdị Ọrịa Iku Oku Iri na Otu

Foto a na-egosipụta ụdị ọrịa iku ume iri na otu

Nkọwa Dị Mkpirikpi:

A na-eji ngwa a maka ịchọpụta nje ndị na-akpata iku ume n'ime ọbara mmadụ, gụnyere Haemophilus influenzae (HI), Streptococcus pneumoniae (SP), Acinetobacter baumannii (ABA), Pseudomonas aeruginosa (PA), Klebsiella Pneumoniae (KPN), Stenotrophomonas maltophilia (Smet), Bordetella pertussis (BP), Bacillus parapertusss (Bpp), Mycoplasma pneumoniae (MP), Chlamydia pneumoniae (Cpn), Legionella pneumophila (Leg). A pụrụ iji nsonaazụ ya mee ihe dị ka enyemaka n'ịchọpụta ndị ọrịa nọ n'ụlọ ọgwụ ma ọ bụ ndị ọrịa siri ike nwere ọrịa nje bacteria nke akụkụ iku ume.A na-eji ngwa a maka ịchọpụta nje ndị na-akpata iku ume n'ime ọbara mmadụ, gụnyere Haemophilus influenzae (HI), Streptococcus pneumoniae (SP), Acinetobacter baumannii (ABA), Pseudomonas aeruginosa (PA), Klebsiella Pneumoniae (KPN), Stenotrophomonas maltophilia (Smet), Bordetella pertussis (BP), Bacillus parapertusss (Bpp), Mycoplasma pneumoniae (MP), Chlamydia pneumoniae (Cpn), Legionella pneumophila (Leg). A pụrụ iji nsonaazụ ya mee ihe dị ka enyemaka n'ịchọpụta ndị ọrịa nọ n'ụlọ ọgwụ ma ọ bụ ndị ọrịa siri ike nwere ọrịa nje bacteria nke akụkụ iku ume.

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Nkọwa Ngwaahịa

Akara Ngwaahịa

Aha ngwaahịa

Ngwa Nchọpụta Acid Nucleic nke HWTS-RT162A Ụdị Ọrịa Ikuku Iri na Otu(PCR fluorescence)

Ọwa

靶标				Ọwa
核酸反应液A	核酸反应液B	核酸反应液C	核酸反应液D	Ọwa
HI	Smet	MP	Ụkwụ	FAM
SP	PA	Bp	/	CY5
KPN	ABA	Bpp	Cpn	ROX
内参	内参	内参	内参	VIC/HEX

Ọmụmụ ihe gbasara ọrịa na-efe efe

Ọrịa iku ume bụ otu n'ime ọrịa ndị dị mkpa nke na-eyi ahụike mmadụ egwu nke ukwuu, ọmụmụ ihe egosila na ọtụtụ ọrịa iku ume na-esite na nje bacteria na nje virus na-ebute ọrịa ahụ, nke na-ebute mmụba nke ọrịa ahụ na ọbụna ọnwụ, yabụ ịkọwa ihe kpatara ọrịa ahụ na-enye ohere maka ọgwụgwọ ezubere iche ma melite ọnụego ndụ nke onye ọrịa.^[1,2].

Usoro Nkà na ụzụ

Nchekwa	≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ihe atụ	Sputum
Ct	HI, SP, KPN, PA, ABA, Smet: Ct≤33BP, Bpp, MP, Cpn, Ụkwụ: Ct≤38
CV	<5.0%
LoD	Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonas.aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia na LegionellaPneumophila: 1000 CFU/mL； Bordetella pertussis na Bacillus parapertussis: 500 CFU/mL; Mkpụrụ ọgwụ Mycoplasma pneumoniae na Chlamydia pneumoniae: 200 mbipụta/mL.
Nkọwapụta	Nnwale nnwale mmeghachi omume gosiri na enweghị mmeghachi omume mmegharị ahụ n'etiti ngwa a na Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, ụdị Mycobacterium tuberculosis attenuated, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivaryus, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiraveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii na asịd nucleic genomic nke mmadụ.
Ngwa Ndị Dị Mkpa	Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio^®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler^®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96

Usoro Ọrụ

Ngwa Nleba Anya Ngwa DNA/RNA nke Ngwa ...physiologicalsaline na ihe e ji eme ihe, a ga-emekwa ihe ndị na-esote dịka ntuziaka si dị. Olu mmiri a tụrụ aro bụ 100 µL.

Nke gara aga: Mgbanwe Mkpụrụ Ndụ Ihe Njikọ PML-RARA nke Mmadụ

Osote: ALDH Mkpụrụ ndụ ihe nketa Polymorphism

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