Ọrịa Ebola nke Zaire
Aha ngwaahịa
HWTS-FE008 Ngwa ihe nchọpụta nje virus nke Zaire Ebola (Fluorescence PCR)
Epidemiology
Ọrịa Ebola bụ nke Filoviridae, nke bụ nje RNA na-adịghị mma nke nwere eriri na-adịghị mma.Nje Virus bụ ogologo filaments nwere nkezi virion ogologo nke 1000nm na dayameta nke ihe dịka 100nm.Ọrịa Ebola genome bụ eriri RNA na-adịghị mma nke nwere oke 18.9kb, na-edobe protein 7 na protein anaghị arụ ọrụ.Enwere ike kewaa nje Ebola n'ụdị dịka Zaire, Sudan, Bundibugyo, Tai Forest na Reston.N'ime ha, ụdị Zaire na ụdị Sudan ka akọọrọ na-ebute ọrịa na-egbu ọtụtụ mmadụ.EHF (Ebola Hemorrhagic Fever) bụ nnukwu ọrịa hemorrhagic na-efe efe nke nje Ebola kpatara.Ndị mmadụ na-ebutekarị ọrịa site na kọntaktị na mmiri ara, ihe nzuzo na excreta nke ndị ọrịa ma ọ bụ anụmanụ ndị bu ọrịa, na ngosipụta ụlọ ọgwụ na-ebutekarị ahụ ọkụ, ọbara ọgbụgba na ọtụtụ mmebi akụkụ ahụ.EHF nwere ọnụ ọgụgụ ọnwụ dị elu nke 50%-90%.
Ọwa
| FAM | MP nucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Ọhụrụ serum, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 oyiri/μL |
| Nkọwapụta | Jiri ngwa ahụ iji nwalee nrụtụ aka ọjọọ nke ụlọ ọrụ, nsonaazụ ya na-emezu ihe achọrọ. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistemụ PCR Real-Time BioRad CFX96, yana BioRad CFX Opus 96 Ezigbo PCR Sistemu |
Usoro ọrụ
Nhọrọ 1.
Reagent mmịpụta akwadoro: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd.Ekwesịrị ịwepụta ya n'ụzọ siri ike dịka ntuziaka ahụ si dị, na oke mmịpụta akwadoro nke sample bụ 140μL na oke elution akwadoro bụ 60μL.
Nhọrọ 2.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).It. kwesiri ibuputa dika ntuziaka.The mmịpụta sample olu bụ 200μL , na akwadoro elution olu bụ 80μL.
Nhọrọ 3.
Na-atụ aro mmịpụta reagents: Nucleic Acid Extraction Reagent (1000020261) na High throughput Automated Sample Preparation System(MGISP-960) nke BGI kwesịrị ka ewepụtara dị ka ntuziaka ahụ si dị.Olu mmịpụta bụ 160μL, na olu elution akwadoro bụ 60μL.
