Ihe isii na-akpata iku ume nke a kpọrọ nkụ na oyi
Aha ngwaahịa
HWTS-RT177-Ngwa Nchọpụta Agụụ Itoolu nke a kpọrọ nkụ nke akpọnwụrụ akpọnwụ. Ngwa Nchọpụta Agụụ Itoolu (Fluorescence PCR)
Nkwado Ngwaahịa
IVDR
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa iku ume bụ ụdị ọrịa mmadụ kachasị na-ebutekarị, nke nwere ike ime na ihe ọ bụla dị na ya.okike, afọ na mpaghara, ọ bụkwa otu n'ime ihe ndị kacha mkpa na-akpata ọrịa na ọnwụ n'ụwa [1]. Ọrịa iku ume a na-ahụkarị n'ụlọ ọgwụ gụnyere nje syncytial respiratory, adenovirus, nje metapneumovirus mmadụ, nje rhinovirus, nje parainfluenza (I/II/III) na mycoplasma pneumoniae, wdg. [2,3]. Ihe mgbaàmà na ihe ịrịba ama nke ọrịa iku ume na-akpata yiri nke ahụ, mana ọrịa nke nje dị iche iche na-akpata nwere ụzọ ọgwụgwọ dị iche iche, mmetụta ọgwụgwọ na usoro ọrịa [4,5]. Ugbu a, ụzọ ndị bụ isi nke nchọpụta ụlọ nyocha nke nje iku ume gụnyere mwepụta nje, nchọpụta antigen na nchọpụta nucleic acid, wdg. Ngwa a na-achọpụta ma na-achọpụta ụfọdụ nucleic acid nje na ndị mmadụ nwere ihe ịrịba ama na ihe mgbaàmà nke ọrịa iku ume, yana nsonaazụ ụlọ ọgwụ na ụlọ nyocha ndị ọzọ iji nyere aka na nchọpụta nke ọrịa iku ume. Ekwesighi iji nsonaazụ nke ule a dị ka naanị ntọala maka nchọpụta, ọgwụgwọ, ma ọ bụ mkpebi njikwa ndị ọrịa ndị ọzọ.
Usoro Nkà na ụzụ
| Nchekwa | 2-28℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | swab oropharyngeal mmadụ, swab nasopharyngeal |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Mbipụta 200/mL |
| Nkọwapụta | Mmeghachi omume n'etiti kit na nje virus nke Human boca, Cytomegalovirus, Herpes simplex virus type 1, Varicella zoster virus, Epstein-barr virus, Bacillus pertussis, Chlamydia pneumoniae, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, Ụdị ụkwara nta mycobacterium attenuated, Neisseria meningitidis, Neisseria spp. (Neisseria gonorrhoeae), Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, IFV A, IFV B, Parainfluenza IV, SARS-CoV-2, Coronavirus (HKU1), Coronavirus (OC43), Coronavirus (NL63). |
| Ngwa Ndị Dị Mkpa | Ụdị nke Mbụ: Sistemụ PCR nke Ejirila Mee 7500 Real-Time, SLAN-96P Sistemụ PCR nke Oge A Na-ahụ Anya (Hongshi Medical Technology Co., Ltd.). Nnwale Ụdị nke Abụọ: EudemonTM AIO800 (HWTS-EQ007) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro Ọrụ
Ụdị nke Mbụ: Na sistemụ PCR nkịtị
Ụdị nke Abụọ: Na EudemonTMAIO800
Mkpa maka reagents/ngwa ọrụ mana enyeghị ya
Ngwa Ngwuputa DNA/RNA nke Macro na Micro-Test Viral (HWTS-3017) (nke enwere ike iji ya na Macro & MicroTest Automatic Nucleic Acid Extractor (HWTS-EQ011)) nke Jiangsu Macro & Micro-Test MedTech Co., Ltd. na Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (eji ya naEudemonTM AIO800) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.Ngwọta nchekwa ihe nlele: 0.9% ngwọta sodium chloride, Ngwa Nnwale Nje Virus (MT0301-1) site naỤlọ ọrụ teknụzụ Yocon Biology (Beijing), UTM® Universal Transport Medium™ (GXB No.: 20191277) site n'aka Copan Italia SpA, Ngwa Nchịkọta Ihe Nlereanya, Ozi na Mbupu (HWTS-3002) site n'aka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
A chọrọ ihe oriri mana enyeghị ha:Ntuziaka na-enweghị DNase/RNase, uwe aka a na-atụfu atụfu, tube EP nke na-enweghị DNase/RNase, ihe na-ewepụta bead magnet ma ọ bụ rack magnet.
