Ụdị 14 nke Human Papillomavirus nwere nnukwu ihe ize ndụ (16/18/52 Mpempe akwụkwọ)
Aha ngwaahịa
HWTS-CC019A-Ụdị 14 nke Papillomavirus mmadụ nwere nnukwu ihe ize ndụ (16/18/52) Ngwa nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Nnyocha egosiwo na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa na-efe efe bụ otu n'ime isi ihe na-ebute ọrịa kansa cervical.Ugbu a, ọgwụgwọ ndị a ma ama dị irè ka enweghị maka ọrịa kansa cervical nke HPV kpatara, yabụ n'oge nchọpụta na mgbochi nke ọrịa cervical nke HPV kpatara bụ isi ihe na-egbochi ọrịa kansa cervical.Ọ dị oke mkpa iji guzobe ule nyocha ọrịa etiology dị mfe, akọwapụtara na ngwa ngwa maka nyocha ụlọ ọgwụ na ọgwụgwọ ọrịa kansa cervical.
Ọwa
| Ọwa | Ụdị |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| Afọ 705/CY5.5 | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Urine, Cervical Swab, ikpu swab |
| Ct | ≤28 |
| LoD | 300 oyiri/ml |
| Nkọwapụta | Ọ dịghị cross-reactivity na ndị ọzọ akụkụ okuku ume samples dị ka Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echomo2 virus, Metapneu. B1/B2, nje syncytial respiratory A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, wdg na mmadụ genomic DNA. |
| Ngwa ndị ọdabara | MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistemụ PCR nke BioRad CFX96 na BioRad CFX Opus 96 ezigbo oge PCR Sistemu |
Usoro ọrụ
1.Urine sample
A: Were1.4mL nke ihe nlele urine ga-anwale na centrifuge na 12000rpm maka nkeji 5;tụfuo ihe dị elu (a na-atụ aro ka idobe 10-20μL supernatant site na ala nke tube centrifuge), gbakwunye 200μL nke reagent ntọhapụ, na mmịpụta na-esote ga-eduzi dịka ntuziaka maka iji Macro & Micro-Test Sample Release. Reagent (HWTS-3005-8).
B: Were1.4mL nke ihe nlele mmamịrị a ga-anwale na centrifuge na 12,000rpm maka nkeji 5;tụfuo ihe dị elu (a na-atụ aro ka idobe 10-20μL nke supernatant site na ala nke tube centrifuge), ma tinye 200μL nke saline nkịtị ka ọ maliteghachi, dị ka ihe nlele a ga-anwale.Enwere ike iduzi mmịpụta na-esote na Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. dị ka ntuziaka siri dị.s maka ojiji.Oke elution akwadoro bụ 80μL.
C: Were1.4mL nke ihe nlele mmamịrị a ga-anwale na centrifuge na 12,000rpm maka nkeji 5;tụfuo ihe dị elu (a na-atụ aro ka idobe 10-20μL nke supernatant site na ala nke tube centrifuge), ma tinye 200μL nke saline nkịtị ka ọ maliteghachi, dị ka ihe nlele a ga-anwale.Enwere ike ịme mmịpụta na-esote ya naNgwa QIAamp DNA Obere (51304) nke QIAGEN ma ọ bụ Makiro & Micro-Test Viral DNA/RNA kọlụm (HWTS-3020-50).Ekwesịrị ịhazi mmịpụta dịka ntuziaka maka ojiji.Ihe nlele nke mmịpụta bụ 200μL, na olu elution akwadoro bụ 80μL.
2. Cervical swab / ikpu swab sample
A: Were 1ml nke ihe nlele ka nwalee na 1.5mLof tube nke centrifuge,nacentrifuge na 12000rpm maka nkeji 5. Discard the supernatant (a na-atụ aro ka idobe 10-20μL nke supernatant site na ala nke tube centrifuge), tinye 100μL nke reagent ntọhapụ sample, wee wepụ dị ka ntuziaka maka ojiji nke Macro & Micro-Test Sample Release Reagent ( HWTS-3005-8).
B: Enwere ike ịme mmịpụta ahụ na Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji ya na Macro). & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. na-agbaso ntuziaka maka ojiji.Ihe nlele nke ewepụtara bụ 200μL, na olu elution akwadoro bụ 80μL.
C: Enwere ike iji QIAamp DNA Mini Kit (51304) mee mmịpụta ahụ site na QIAGEN ma ọ bụ Makiro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ekwesịrị ịhazi mmịpụta dịka ntuziaka maka ojiji.Ihe nlele nke mmịpụta bụ 200 μL, na olu elution akwadoro bụ80 μl.
3, Cervical Swab/Ikpu Swab
Tupu ị na-eme nlele, jiri swab owu jiri nwayọọ hichapụ ihe nzuzo na-adịghị na cervix, ma jiri swab owu ọzọ nke etinyere na ngwọta nchekwa cell ma ọ bụ brush cervical exfoliated cell sampling sampling ka ịrapara na mucosa cervical wee tụgharịa elekere 3-5 na elekere iji nweta. cell exfoliated cervical.Jiri nwayọ wepụta swab ma ọ bụ ahịhịa owu,natinye ya na tube nke nwere 1 ml nke saline nkịtị na-adịghị ọcha. AMgbe ịsachara nke ọma, kpochaa swab owu ma ọ bụ ahịhịa na mgbidi tube wee tụfuo, mee ka okpu tube sie ike, ma kaa aha nlele (ma ọ bụ nọmba) wee pịnye na tube sample.
