Mycobacterium ụkwara nta Nucleic Acid na Rifampicin Nguzogide
Aha ngwaahịa
HWTS-RT074B-Mycobacterium ụkwara nta Nucleic Acid na Rifampicin Mgbochi Nchọpụta Ngwa (Melting Curve)
Asambodo
CE
Epidemiology
Mycobacterium ụkwara nta, obere oge dị ka Tubercle bacillus, TB, bụ nje nje na-akpata ụkwara nta.Ugbu a, ọgwụ mgbochi ụkwara nta nke mbụ a na-ejikarị gụnyere isoniazid, rifampicin na hexambutol, wdg. Ọgwụ mgbochi ụkwara nta nke abụọ gụnyere fluoroquinolones, amikacin na kanamycin, wdg. Ọgwụ ọhụrụ emepụtara bụ linezolid, bedaquiline na delamani, wdg. Otú ọ dị, n'ihi iji ọgwụ mgbochi ụkwara nta na-ezighị ezi na njirimara nke mgbidi cell nke ụkwara nta nke mycobacterium, ụkwara nta mycobacterium na-emepụta ọgwụ na-eguzogide ọgwụ mgbochi ụkwara nta, nke na-eweta nnukwu nsogbu na mgbochi na ọgwụgwọ ụkwara nta.
A na-eji Rifampicin na-agwọ ndị ọrịa ụkwara nta nke akpa ume kemgbe afọ 1970, ma nwee mmetụta dị ukwuu.Ọ bụ nhọrọ mbụ iji belata ọgwụgwọ ọgwụgwọ nke ndị ọrịa ụkwara nta.Ihe na-akpata nguzogide Rifampicin bụ ngbanwe nke mkpụrụ ndụ ihe nketa rpoB.Ọ bụ ezie na ọgwụ ọhụrụ na-egbochi ụkwara nta na-apụta mgbe nile, na ịdị irè ụlọ ọgwụ nke ndị ọrịa ụkwara nta na-aga n'ihu na-agakwa n'ihu, a ka nwere ụkọ ọgwụ mgbochi ụkwara nta, na ọdịdị nke iji ọgwụ na-enweghị isi eme ihe na ụlọ ọgwụ dị oke elu.N'ụzọ doro anya, ọrịa ụkwara nta nke Mycobacterium nke ndị ọrịa nwere ụkwara nta akpa ume enweghị ike igbu kpamkpam n'oge kwesịrị ekwesị, nke na-emecha na-eduga n'ọkwa dị iche iche nke nguzogide ọgwụ n'ime ahụ onye ọrịa, na-agbatị usoro nke ọrịa ahụ, na-abawanye ohere nke ọnwụ nke onye ọrịa.
Ọwa
| Ọwa | Ọwa na Fluorophores | Ihe nchekwa mmeghachi omume A | Ihe nchekwa mmeghachi omume B | Ihe nchekwa mmeghachi omume C |
| Ọwa FAM | Onye nta akụkọ: FAM, Quencher: Ọ dịghị | rpoB 507-514 | rpoB 513-520 | 38KD na IS6110 |
| Ọwa CY5 | Onye nta akụkọ: CY5, Quencher: Ọ dịghị | rpoB 520-527 | rpoB 527-533 | / |
| Ọwa HEX (VIC). | Onye nta akụkọ: HEX (VIC), Quencher: Ọ dịghị | Njikwa ime | Njikwa ime | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ N'ọchịchịrị |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Sputum |
| CV | ≤5.0% |
| LoD | mycobacterium ụkwara nta 50 bacteria / ml Ụdị anụ ọhịa rifampicin na-eguzogide: 2x103nje bacteria/ml mutant homozygous: 2x103nje bacteria/ml |
| Nkọwapụta | Ọ na-achọpụta ụkwara nta mycobacterium nke anụ ọhịa na ebe ngbanwe nke mkpụrụ ndụ ihe nketa ọgwụ ndị ọzọ dị ka katG 315G>C\A, InhA-15C> T, nsonaazụ ule egosighi nguzogide rifampicin, nke pụtara na ọ dịghị cross-reactivity. |
| Ngwa ngwa: | Sistemụ PCR SLAN-96P ezigbo oge BioRad CFX96 ezigbo oge PCR sistemụ LightCycler480® Real-Time PCR System |
Usoro ọrụ
Ọ bụrụ na-eji Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (nke enwere ike iji ya na Macro & Micro-Test Automatic. Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ma ọ bụ Macro & Micro-Test Viral DNA/RNA kọlụm (HWTS-3022-50) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. maka mmịpụta, tinye 200μL nke njikwa dị mma, njikwa na-adịghị mma na nhazi sputum sample ga-anwale n'usoro, ma tinye 10μL nke njikwa n'ime ya iche n'ime njikwa ziri ezi, njikwa na-adịghị mma na nhazi sputum sample ga-anwale, na usoro ndị na-esote kwesịrị ime nke ọma. dị ka ntuziaka mmịpụta.Ihe nlele nke ewepụtara bụ 200μL, na olu elution akwadoro bụ 100μL.
