Ụdị iri na anọ nke HPV Nucleic Acid Type

Ụdị iri na anọ nke HPV Nucleic Acid Ide Ihe Foto A Na-egosipụta

Nkọwa Dị Mkpirikpi:

Ọrịa Papillomavirus nke mmadụ (HPV) sitere n'ezinụlọ Papillomaviridae nke obere molekul, nke na-abụghị nke e kpuchiri ekpuchi, nke nwere DNA gbara okirikiri nke nwere eriri abụọ, nke nwere ogologo genome nke ihe dị ka pairs isi 8000 (bp). Ọrịa HPV na-ebute ọrịa site na kọntaktị kpọmkwem ma ọ bụ na-apụtaghị ìhè na ihe ndị ruru unyi ma ọ bụ nnyefe mmekọahụ. Ọrịa a abụghị naanị nke onye ọbịa, kamakwa nke metụtara anụ ahụ, ọ nwere ike ibute naanị akpụkpọ ahụ mmadụ na mkpụrụ ndụ epithelial mucosal, na-akpata ọtụtụ papillomas ma ọ bụ warts na akpụkpọ ahụ mmadụ ma na-emebi epithelium tract tract.

Ngwa a dabara adaba maka nchọpụta ụdị n'ime vitro nke ụdị nje papillomavirus iri na anọ nke mmadụ (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) nucleic acids dị na mmamịrị mmadụ, ihe nlele swab cervical nwanyị, na ihe nlele swab ikpu nwanyị. Ọ nwere ike inye naanị ụzọ enyemaka maka nchọpụta na ọgwụgwọ nke ọrịa HPV.

Zitere anyị ozi-e

Nkọwa Ngwaahịa

Akara Ngwaahịa

Aha ngwaahịa

Ụdị HWTS-CC012A-14 nke Ngwa Nchọpụta Mpempe Asịd HPV Nucleic (Fluorescence PCR)

HWTS-CC021-Kpọchara akpọnwụ Ụdị Ngwa Nchọpụta Ngụkọta Acid Nucleic nke Papillomavirus Mmadụ Iri na Anọ (Fluorescence PCR)

Asambodo

Ọmụmụ ihe gbasara ọrịa na-efe efe

Ọrịa kansa olu bụ otu n'ime etuto ọjọọ kachasị na-apụta n'ime ụmụ nwanyị. Ọmụmụ ihe ndị a egosila na ọrịa na-adịgide adịgide na ọtụtụ ọrịa nke papillomavirus mmadụ bụ otu n'ime ihe ndị dị mkpa na-akpata kansa olu. Ugbu a, enwere enweghị usoro ọgwụgwọ dị irè a ma ama maka HPV. Ya mere, nchọpụta ngwa ngwa na mgbochi HPV olu bụ isi ihe na-egbochi kansa. Ịtọlite usoro nchọpụta ọrịa dị mfe, nke a kapịrị ọnụ na nke ngwa ngwa dị oke mkpa na nchọpụta ọrịa kansa olu.

Ọwa

FAM	HPV16, 58, ntụaka dị n'ime
VIC (HEX)	HPV18, 33, 51, 59
CY5	HPV35, 45, 56, na 68
ROX	HPV31, 39, 52, 66

Usoro Nkà na ụzụ

Nchekwa	≤-18℃ N'ọchịchịrị
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ihe atụ	Mmamịrị, swab nke eriri afọ, swab nke ikpu
Ct	≤28
CV	<5.0%
LoD	300Mpempe akwụkwọ/mL
Ngwa Ndị Dị Mkpa	Ọ nwere ike ịha nhata ngwa PCR fluorescent kachasị ewu ewu n'ahịa.Sistemụ PCR SLAN-96P Oge-N'ezie Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere QuantStudio^®Sistemụ PCR 5 n'oge a na-arụ ọrụ LightCycler^®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya