Ụdị Ngwa Nchọpụta Asịd HPV Nucleic 28 (Fluorescence PCR)
Aha ngwaahịa
Ụdị Ngwa Nchọpụta Asịd HPV Nucleic 28 (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Papillomavirus nke mmadụ (HPV) sitere n'ezinụlọ Papillomaviridae nke obere molekul, nke na-abụghị nke e kpuchiri ekpuchi, nke nwere DNA gbara okirikiri nke nwere eriri abụọ, nke nwere ogologo genome nke ihe dị ka pairs isi 8000 (bp). Ọrịa HPV na-ebute ọrịa site na kọntaktị kpọmkwem ma ọ bụ na-apụtaghị ìhè na ihe ndị ruru unyi ma ọ bụ nnyefe mmekọahụ. Ọrịa a abụghị naanị nke onye ọbịa, kamakwa nke metụtara anụ ahụ, ọ nwere ike ibute naanị akpụkpọ ahụ mmadụ na mkpụrụ ndụ epithelial mucosal, na-akpata ọtụtụ papillomas ma ọ bụ warts na akpụkpọ ahụ mmadụ ma na-emebi epithelium tract tract.
Ọrịa kansa olu bụ otu n'ime etuto ọjọọ kachasị na-eme n'ime ụmụ nwanyị. Nnyocha egosila na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa bụ otu n'ime ihe ndị bụ isi na-akpata ọrịa kansa olu. Ugbu a, a ka nwere ọgwụgwọ dị irè a ma ama maka kansa olu nke HPV kpatara, yabụ nchọpụta mbụ na mgbochi nke ọrịa olu nke HPV kpatara bụ isi ihe na-egbochi kansa olu. Ọ dị oke mkpa ịmepụta ule nchọpụta etiology dị mfe, kpọmkwem na ngwa ngwa maka nchọpụta ahụike na ọgwụgwọ nke kansa olu.
Ọwa
| S/N | Ọwa | Ụdị |
| PCR-Mix1 | FAM | 16, 18, 31, 56 |
| VIC (HEX) | Njikwa Dị n'ime | |
| CY5 | 45, 51, 52, 53 | |
| ROX | 33, 35, 58, 66 | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC (HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| ROX | 39, 59, 68, 73 |
Usoro Nkà na ụzụ
| Nchekwa | ≤ -18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Ihe nlele mmamịrị, ihe nlele akpụkpọ ahụ nke nwanyị, ihe nlele akpụkpọ ahụ nke nwanyị |
| CV | <5.0% |
| LoD | Mbipụta 300/mL |
| Nkọwapụta | Enweghị mmeghachi omume cross-reactions na nje ndị ọzọ metụtara usoro ọmụmụ na DNA genomic mmadụ. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'oge Ejiri Eme Ihe nke Biosystems 7500, Sistemụ PCR Oge-N'oge Ngwa Ngwa nke Biosystems 7500, Sistemụ PCR Oge-N'oge Ngwa Ngwa nke QuantStudio®5, Sistemụ PCR Oge-N'oge Ngwa nke SLAN-96P (Hongshi Medical Technology Co., Ltd.), Sistemụ PCR Oge-N'oge Ngwa nke LightCycler®480, Sistemụ Nchọpụta Oge-N'oge Ngwa nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ PCR Oge-N'oge Ngwa nke BioRad CFX96, Sistemụ PCR Oge-N'oge Ngwa nke BioRad CFX Opus 96. |
Usoro ọrụ: Lee Kit IFU maka nkọwa ndị ọzọ
Ngwọta nke 1 (Mwepụta akpaghị aka nke nucleic acid)
Ngwọta nke 2: (Macro & Micro-Ule Sample Release Reagent)
Ihe ndị na-eme ka ihe na-eme ka ihe na-eme ka ihe dị mma chọrọ mana enyeghị ya
Ngwa Mwepụta Ihe Nlereanya Maka Nlereanya Macro na Micro (HWTS-3005-8), Ngwa Ngwa DNA/RNA Maka Nlereanya Macro na Micro (HWTS-3017) (nke enwere ike iji ya na Ngwa ...
A chọrọ ihe oriri mana a naghị enye ha
1.5mL nke ọkpọkọ DNase/RNase na-enweghị, tips na-enweghị DNase/RNase, mpempe tube 8 maka PCR, centrifuge tebụl, igwekota ihe na-agwakọta tebụl.
