Ụdị 15 nke nje virus mmadụ nwere ike ibute nnukwu nsogbu E6/E7 mRNA
Aha ngwaahịa
Ụdị HWTS-CC005A-15 nke Papillomavirus mmadụ dị oke egwu E6/E7 Gene Detection Kit (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa kansa olu bụ otu n'ime ụdị kansa ụmụ nwanyị kachasị ahụkarị n'ụwa niile, ihe na-eme ya na-ejikọkwa ya na nje virus papilloma mmadụ (HPV), mana naanị obere akụkụ nke ọrịa HPV nwere ike ịghọ kansa. HPV dị oke ize ndụ na-ebute ọrịa na mkpụrụ ndụ epithelial olu ma na-emepụta oncoproteins abụọ, E6 na E7. Protein a nwere ike imetụta ọtụtụ protein sel (dịka protein ndị na-egbochi etuto ahụ pRB na p53), na-agbatị usoro sel, na-emetụta njikọ DNA na nkwụsi ike genome, ma na-egbochi nzaghachi mgbochi nje na mgbochi etuto ahụ.
Ọwa
| Ọwa | Akụkụ | A nwalere ụdị mkpụrụ ndụ ihe nketa |
| FAM | Nchekwa Mmeghachi Omume HPV 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Mkpụrụ ndụ ihe nketa β-actin mmadụ | |
| FAM | Nchekwa Mmeghachi Omume HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Mkpụrụ ndụ ihe nketa mmadụ (INS) |
Usoro Nkà na ụzụ
| Nchekwa | Mmiri mmiri: ≤-18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | Swab nke akpanwa |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Mbipụta 500/mL |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyereSistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa Sistemụ PCR nke QuantStudio®5 n'oge dị adị Sistemụ PCR SLAN-96P Oge-N'ezie Sistemụ PCR oge-oge LightCycler®480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Ihe na-eme ka a na-ewepụta ihe a tụrụ aro: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. A ga-eme mwepụta ahụ nke ọma dịka ntuziaka maka ojiji si dị. Olu mwepụta a tụrụ aro bụ 50μL. Ọ bụrụ na ihe nlele ahụ agbazeghị kpamkpam, weghachite ya na nzọụkwụ nke 4 maka ịmịnyeghachi ya. Wee nwalee ya dịka ntuziaka maka ojiji si dị.
Ihe na-eme ka a na-ewepụta ihe: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). A ga-eme mwepụta ahụ dịka ntuziaka maka ojiji siri dị (Na nzọụkwụ nke 5, jiri okpukpu abụọ nke ihe ngwọta DNaseI na-arụ ọrụ, ya bụ, were 20μL nke ihe ngwọta RNase-Free DNaseI (1500U) n'ime tube centrifuge RNase-Free ọhụrụ, tinye 60μL nke ihe nchekwa RDD, wee gwakọta ya nwayọ). Olu mgbapụta akwadoro bụ 60μL. Ọ bụrụ na ihe nlele ahụ agbazeghị kpamkpam, weghachite ya na nzọụkwụ nke 5 maka ịmịgharị ya ọzọ. Wee nwalee ya dịka ntuziaka maka ojiji si dị.
