Ụdị 15 nke Papillomavirus mmadụ nwere nnukwu ihe ize ndụ E6/E7 Gene mRNA
Aha ngwaahịa
HWTS-CC005A-15 Ụdị nke Papillomavirus mmadụ nwere nnukwu ihe ize ndụ E6/E7 Gene mRNA nchọpụta ngwa (Fluorescence PCR)
Epidemiology
Ọrịa kansa cervical bụ otu n'ime ụdị ọrịa kansa ụmụ nwanyị na-ahụkarị n'ụwa niile, na ihe na-eme ya nwere njikọ chiri anya na nje papillomavirus mmadụ (HPV), mana ọ bụ naanị obere akụkụ nke ọrịa HPV nwere ike ịmalite ịghọ kansa. HPV nwere nnukwu ihe ize ndụ na-ebute mkpụrụ ndụ epithelial cervical ma mepụta oncoproteins abụọ, E6 na E7. Protein a nwere ike imetụta ụdị protein cellular dị iche iche (dị ka protein suppressor pRB na p53), mee ka usoro cell dịkwuo ogologo, na-emetụta njikọ DNA na nkwụsi ike genome, ma na-egbochi nzaghachi mgbochi nje na antitumor.
Ọwa
| Ọwa | Akụkụ | A nwalere Genotype |
| FAM | Ihe nchekwa mmeghachi omume HPV 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Mkpụrụ ndụ β-actin mmadụ | |
| FAM | Ihe nchekwa mmeghachi omume HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Mkpụrụ ndụ mmadụ INS |
Nka nka
| Nchekwa | Mmiri: ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Cervical swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 oyiri/ml |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCRNgwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR Real-Time QuantStudio®5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The mmịpụta ga-eduzi nke ọma dị ka ntuziaka maka ojiji. Oke elution akwadoro bụ 50μL. Ọ bụrụ na ihe nlele ahụ adịghị agbaze kpamkpam, weghachite ya na nzọụkwụ 4 maka rerigesting. Ma nwalee dịka ntuziaka maka ojiji.
Reagent mmịpụta akwadoro: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Ekwesịrị ịme mmịpụta ahụ dị ka ntuziaka maka ojiji siri ike (Na nzọụkwụ 5, okpukpu abụọ nke itinye uche nke DNaseI na-arụ ọrụ ngwọta, ya bụ, were 20μL nke RNase-Free DNaseI (1500U) ngwaahịa ngwaahịa n'ime tube centrifuge ọhụrụ RNase-Free, tinye 60μL nke ihe nchekwa RDD, wee gwakọta nwayọ). Oke elution akwadoro bụ 60μL. Ọ bụrụ na ihe nlele ahụ agbazeghị kpamkpam, weghachite ya na nzọụkwụ 5 maka ịmegharị. Ma nwalee dịka ntuziaka maka ojiji.
