15dị ụdị iri na ise nke ihe egwu na-egwu mmadụ
Aha
HWT-CC005A-15 nke ihe dị egwu na-egwu mmadụ papillomavirus e6 / E7 Gene Mrna nchọpụta Kit (Fluorescence Pc)
Mpidermiology
Cersical cercer bụ otu n'ime ụdị ndị a na-ahụkarị n'ụwa niile, ihe a na-ahụkarị metụtara ya na ọrịa papillomavirs (HPV), mana ọ bụ naanị obere ọrịa HPV nwere ike ịta ahụhụ kansa. HPV na-eme ka akwara dị elu ma na-emepụta oncroprotein abụọ, E6 na E7. Protein a nwere ike imetụta protein protein (dị ka ndị nka ime protein proteins pscle, metụta DNA na-akwụsi ike, ma na-egbochi nzaghachi antiviral na ihe ndị a na-ahụ maka antiviral na nke antitation na antiline.
Chaneelu
| Chaneelu | Anuahu | Anwalele ule |
| M | HPV mmeghachi omume 1 | HPV16,31,33,35,51,52,52 |
| Vic / hex | Mmadụ β-actin Gene | |
| M | HPV mmeghachi omume 2 | HPV 18,39,45,53,56,6,6,6,68 |
| Vic / hex | Onyinye Onyinye |
Nrụta Nka na ụzụ
| Storaji | Mmiri: ≤-18 ℃ |
| Ebe ndu | Ọnwa 9 |
| Ụdị ụdị | Swab swab |
| Ct | ≤38 |
| CV | <5.0% |
| Loo | 500 mbipụta / ml |
| Ngwá agha | Etinyere Biosystems 7500 ezigbo oge pcr sistemụNgwa Biosystems na-etinye 7500 ngwa ngwa usoro pcr Systrudio®5 ezigbo oge pcr Slan-96p ezigbo oge pcr Lightcycler®480 Real System System Linegene 9600 Pụnyere Real Chemical System Ma-6000 Real-Oge lyr Beired cfx96 ezigbo oge pcr sistemụ Beired cfx opus 96 Real System |
Na-arụ ọrụ
Mwepu a na-atụ aro ya: macro & micro-nwa agbọghọ (HWT-3020-50-HPV15) site n'aka Jiangsu & Micro-Tech-Tech-Tecro-Tech-Tech. A ga-eduzi mmịpụta dị ka ntuziaka maka iji ya . Olu akwadoro aro bụ 50μL. Ọ bụrụ na ihe nlele ahụ agbarila, weghachi ya na nzọụkwụ 4 maka ime mgbanwe. Wee nwalee dị ka ntuziaka maka iji.
Akwadoro mmịpụta ntụgharị: RNAPREP dị ọcha anụmanụ na-ahụ maka mkpụrụ akụ Rna (DP431). A ga-eduzi mmịpụta ahụ dịka ntuziaka maka iji ya (na nzọụkwụ 5, okpukpu abụọ nke ngwọta Dnasei (1500U) na-enweghị atụ na-enweghị atụ, Tinye 60μL nke RDD Buffer, ma gwakọta nwayọ). Olu akwadoro aro bụ 60μL. Ọ bụrụ na ihe nlele ahụ agbarila, weghachi ya na nzọụkwụ 5 maka ime mgbanwe. Wee nwalee dị ka ntuziaka maka iji.
