Ụdị 15 nke Papillomavirus mmadụ nwere nnukwu ihe ize ndụ E6/E7 Gene mRNA
Aha ngwaahịa
HWTS-CC005A-15 Ụdị nke Papillomavirus mmadụ nwere nnukwu ihe ize ndụ E6/E7 Gene mRNA nchọpụta ngwa (Fluorescence PCR)
Epidemiology
Ọrịa kansa cervical bụ otu n'ime ụdị ọrịa kansa ụmụ nwanyị na-ahụkarị n'ụwa niile, na ihe na-eme ya nwere njikọ chiri anya na nje papillomavirus mmadụ (HPV), mana ọ bụ naanị obere akụkụ nke ọrịa HPV nwere ike ịmalite ịghọ kansa.HPV nwere nnukwu ihe ize ndụ na-ebute mkpụrụ ndụ epithelial cervical ma mepụta oncoproteins abụọ, E6 na E7.Protein a nwere ike imetụta ụdị protein cellular dị iche iche (dị ka protein suppressor pRB na p53), mee ka usoro cell dịkwuo ogologo, na-emetụta njikọ DNA na nkwụsi ike genome, ma na-egbochi nzaghachi antiviral na antitumor.
Ọwa
| Ọwa | Akụkụ | A nwalere Genotype |
| FAM | Ihe nchekwa mmeghachi omume HPV 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Mkpụrụ ndụ β-actin mmadụ | |
| FAM | Ihe nchekwa mmeghachi omume HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Mkpụrụ ndụ mmadụ INS |
Nka nka
| Nchekwa | Mmiri: ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Cervical swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 oyiri/ml |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCRNgwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The mmịpụta ga-eduzi nke ọma dị ka ntuziaka maka ojiji. .Oke elution akwadoro bụ 50μL.Ọ bụrụ na ihe nlele ahụ adịghị agbaze kpamkpam, weghachite ya na nzọụkwụ 4 maka rerigesting.Ma nwalee dịka ntuziaka maka ojiji.
Reagent mmịpụta akwadoro: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Ekwesịrị ịme mmịpụta ahụ dị ka ntuziaka maka ojiji siri ike (Na nzọụkwụ 5, okpukpu abụọ nke itinye uche nke DNaseI na-arụ ọrụ ngwọta, ya bụ, were 20μL nke RNase-Free DNaseI (1500U) ngwọta ngwaahịa n'ime tube centrifuge ọhụrụ RNase-free, tinye 60μL nke ihe nchekwa RDD, ma gwakọta nwayọọ).Oke elution akwadoro bụ 60μL.Ọ bụrụ na ihe nlele ahụ adịghị agbaze kpamkpam, weghachite ya na nzọụkwụ 5 maka rerigesting.Ma nwalee dịka ntuziaka maka ojiji.
