Ụdị 18 nke Nje Virus Nucleic Acid Papilloma nke mmadụ nwere nnukwu ihe ize ndụ
Aha ngwaahịa
Ụdị HWTS-CC018B-18 nke ngwa ihe nchọpụta papilloma nke mmadụ nwere ihe ize ndụ dị elu (Fluorescence PCR)
Asambodo
CE
Epidemiology
Ọrịa kansa cervical bụ otu n'ime etuto ọjọọ na-adịkarị na traktị ọmụmụ ụmụ nwanyị.Nnyocha e mere egosiwo na ọrịa na-adịgide adịgide na ọtụtụ ọrịa papillomavirus mmadụ bụ otu n'ime ihe ndị dị mkpa na-ebute ọrịa kansa cervical.
Ọrịa HPV na-amụ nwa bụ ihe a na-ahụkarị n'etiti ụmụ nwanyị nwere ndụ mmekọahụ.Dị ka ọnụ ọgụgụ si kwuo, pasent 70 ruo 80 nke ụmụ nwanyị nwere ike ịrịa ọrịa HPV otu ugboro ma ọ dịkarịa ala n'oge ndụ ha, mana ọtụtụ ọrịa na-ebute onwe ha, ihe karịrị 90% nke ndị inyom bu ọrịa ga-emepụta nzaghachi dị mma nke na-adịghị ahụkebe nke nwere ike ikpochapụ ọrịa ahụ. n'etiti ọnwa 6 na 24 na-enweghị enyemaka ahụike ogologo oge.Ọrịa HPV nwere nnukwu ihe ize ndụ na-adịgide adịgide bụ isi ihe kpatara neoplasia intraepithelial cervical na ọrịa kansa cervical.
Nsonaazụ ọmụmụ ihe zuru ụwa ọnụ gosiri na ahụrụ ọnụnọ nke HPV DNA dị ize ndụ na 99.7% nke ndị ọrịa cancer cervical.Ya mere, nchọpụta mmalite na mgbochi nke HPV cervical bụ isi ihe na-egbochi ọrịa cancer.Ịmepụta usoro nchọpụta ọrịa dị mfe, kpọmkwem na ngwa ngwa dị oke mkpa na nchọpụta ụlọ ọgwụ nke ọrịa cancer cervical.
Ọwa
| FAM | HPV 18 |
| VIC (HEX) | HPV 16 |
| ROX | HPV 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82 |
| CY5 | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ n'ọchịchịrị |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Cervical swab, ikpu swab, mmamịrị |
| Ct | ≤28 |
| CV | ≤5.0％ |
| LoD | 300 oyiri/ml |
| Nkọwapụta | (1) Ihe na-akpakọrịta Jiri ngwa ahụ nwalee ihe ndị a na-etinye aka na ya, nsonaazụ ya niile adịghị mma: hemoglobin, ọbara ọcha, imi cervical, metronidazole, ude mmiri Jieryin, Fuyanjie ude mmiri, mmanu mmadụ.(2) Cross-reactivity Jiri ngwa ahụ nwalee nje ndị ọzọ metụtara traktị ọmụmụ ọmụmụ yana DNA genomic mmadụ nwere ike inwe mmeghachi omume na ngwa ngwa, nsonaazụ ya niile adịghị mma: ihe nlele HPV6 dị mma, ihe nlele HPV11 dị mma, ihe nlele HPV40 dị mma, ihe nlele HPV42 dị mma, ihe nlele HPV43 dị mma. , HPV44 nti samples, HPV54 mma samples, HPV67 mma samples, HPV69 ziri ezi samples, HPV70 ziri ezi samples, HPV71 ziri ezi samples, HPV72 nti samples, HPV81 nti samples, HPV83 ziri ezi samples, herpes simplex nje ụdị Ⅱ, treponema pallidum, ureacoplasma ureal hominis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, chlamydia trachomatis na mmadụ genomic DNA. |
| Ngwa ndị ọdabara | Sistemụ PCR SLAN-96P ezigbo oge Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Ngwọta PCR zuru oke
Nhọrọ 1.
1. Nlereanya
2. Nucleic acid mmịpụta
3. Tinye ihe nlele na igwe
Nhọrọ 2.
1. Nlereanya
2. Na-enweghị mmịpụta
3. Tinye ihe nlele na igwe
