Ụdị 28 nke HPV Nucleic Acid
Aha ngwaahịa
Ụdị HWTS-CC003A-28 nke HPV Nucleic Acid Detection Kit (Fluorescence PCR)
Asambodo
CE
Epidemiology
Ọrịa kansa cervical bụ otu n'ime etuto ọjọọ na-adịkarị na traktị ọmụmụ ụmụ nwanyị.Ọmụmụ ihe ahụ egosila na ọrịa na-aga n'ihu na ọtụtụ ọrịa papillomavirus mmadụ bụ otu n'ime ihe ndị dị mkpa na-ebute ọrịa kansa cervical.Ka ọ dị ugbu a, a ka enwebeghị usoro ọgwụgwọ dị mma maka HPV.Ya mere, nchọpụta mmalite na mgbochi mbụ nke HPV cervical bụ isi ihe na-egbochi ọrịa cancer.Ịmepụta usoro nchọpụta ọrịa dị mfe, kpọmkwem na ngwa ngwa dị oke mkpa na nchọpụta ụlọ ọgwụ nke ọrịa cancer cervical.
Ọwa
| S/N | Ọwa | Ụdị |
| PCR-Mix1 | FAM | 16, 18, 31, 56 |
| VIC(HEX) | Njikwa ime | |
| CY5 | 45, 51, 52, 53 | |
| ROX | 33, 35, 58, 66 | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| ROX | 39, 59, 68, 73 |
Nka nka
| Nchekwa | ≤-18℃ n'ọchịchịrị |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Selụ exfoliated cervical |
| Ct | ≤28 |
| CV | ≤5.0 nkeji |
| LoD | 300 oyiri/ml |
| Ngwa ndị ọdabara | Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. SLAN ® -96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), Ngwa Biosystem 7500 ezigbo oge PCR, QuantStudio™ 5 Sistemụ PCR ezigbo oge, LightCycler® 480 PCR ezigbo oge, Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 PCR ezigbo oge. |
Ngwọta PCR zuru oke
Nhọrọ 1.
Nhọrọ 2.
