Carbapem iguzogide (KPC / NDM / OXA 48 / Onela 23 / Vim / Mkpu)
Aha
HWT-OT045 na-eguzogide (KPC / NDM / OXA 48 / OXA 23 / Vim / Mkpu)
Mpidermiology
Carbapem nje ọgwụ na-arịginical Β-lactam nke nwere ihe dị iche iche a na-ahụkarị nje na-egbochi nje. N'ihi nkwụsi ike ya na β-lactame na oke oke egwu, ọ ghọrọ otu n'ime ọgwụ nje kachasị mkpa maka ọgwụgwọ nke ọrịa nje siri ike. Carbapen na-akwụsi ike na plasmid - atted gbatịrị-iche iche Β-lactames (esbls), na cephaloporinases (ampy enerated).
Chaneelu
| Pcr-agwakọta 1 | Pcr-mix 2 | |
| M | Iksi ume | Vim |
| Vic / hex | Nchịkwa ime | Nchịkwa ime |
| Pur5 | Mdm | Kpc |
| Rox | OXA48
| Oxa23 |
Nrụta Nka na ụzụ
| Storaji | ≤-18 ℃ |
| Ebe ndu | 12 ọnwa |
| Ụdị ụdị | Sputum, ógbè dị ọcha, agbanyeghị swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Loo | 103Cfu / ml |
| Nnoshi | A) Kit na-achọpụta ihe ndị na-adịghị mma ụlọ ọrụ na-adịghị mma, nsonaazụ ahụ na-emezu ihe ndị a chọrọ nke ederede kwekọrọ. b) Nsonaazụ nke nnwale a na-aga n'ihu na-egosi na ngwa a enweghị mmeghachi omume na nje ndị ọzọ na-efe efe, Pseudoconas Augunosa, Pseudoconas Bereugididis, onye staphyloccolus, klebsicolaella oxytoca, , Prometobacs na-emetụta, na-eme ihe nkiri, na-eme ihe ngosi, ma ọ bụ ihe nlele nke ndị ọzọ na-eguzogide ọgwụ na-adịgide adịgide, na-eme ya, SHV, tem, wdg. c) Ntụgharị aka: Mucin, Minocycline, Minicycinline, Mozis, Clindipenem, Clanehrominine, Clanehrominine, na nsonaazụ na-egosi na ihe ndị a kpọtụrụ aha enweghị mmeghachi omume na nchọpụta nke CABAPAPEM na-emegide mkpụrụ ndụ mkpụrụ ndụ mkpụrụ ndụ mkpụrụ ndụ mkpụrụ ndụ mkpụrụ osisi. |
| Ngwá agha | Ngwa Biosystems na-etinye 7500 ezigbo oge pcr Systrudio®5 ezigbo oge pcr Oge Slan-96p Real-96p Systems (Hongshili Medical Teknụzụ CO., LTD.) Lightcycler®480 Real System System Linegene 9600 gbakwunyere ezigbo oge Pcr nchọpụta (FQD-96A,HangzhouTeknụzụ Bioeer) Ma-6000 Real-Oge gwọike Cycler (Suzhou Molerray Co., LTD.) Beired cfx96 ezigbo oge pcr sistemụ Beired cfx opus 96 Real System |
Na-arụ ọrụ
Nke 1.
A na-atụ aro mmịpụta akwụkwọ ntụgharị: macro & micro-nnwale General DNA / RNA-3019-50, hwts-3019-32, HWT-3019-48, HWT-3019-96) (nke enwere ike iji ya macro-micro-mebere nnwale acid na-emepụta (HWT-3006C, HWT-3007B)) site na 3006b)) na-eme ka ndị na-eme egwuregwu. Usoro ndị sochirinụ kwesịrị iso ntuziaka maka mmịpụta, yana ụda olu na-atụ aro100μl.
Nke 2.
A na-atụ aro mmịpụta ntụgharị: acid acid ma ọ bụ mkpụrụ ndụ dị ọcha (YDP302 Biotech (Beijing) Co. , na ịma jijiji ruo mgbe a na-akwụsịkarị Thallus kpamkpam). Jiri Rnase / Dnates Free maka ịtụgharị uche, Olu econded na-enye ihe dị 100μl.
Nke 3.
A na-atụ aro mmịpụta ntụgharị: macro & micro-lever lever. Ihe atụ nke sputum kwesịrị ịsacha site na ịgbakwunye 1ml nke saline zuru oke na-emeso Thallus Fored Forwaipinned, centrant / minit na-atụfu (Debe 10-20μL nke oke). Maka mpaghara dị ọcha na mpaghara swab, gbakwunye 50μL nke semple depụtara ozugbo na usoro ndị a ga-ewepụta na-emepụta ihe dị ka ntuziaka ahụ si dị.
