Mkpụrụ ndụ ihe nketa Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Aha ngwaahịa
Ngwa Nchọpụta HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọgwụ nje Carbapenem bụ ọgwụ nje β-lactam a na-ahụkarị nke nwere ụdị nje bacteria kachasị obosara na ọrụ nje bacteria kachasị ike. N'ihi nkwụsi ike ya na β-lactamase na obere nsí, ọ aghọọla otu n'ime ọgwụ nje bacteria kachasị mkpa maka ọgwụgwọ ọrịa nje bacteria siri ike. Carbapenems na-adịgide nke ukwuu na plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, na plasmid-mediated cephalosporinases (enzymes AmpC).
Ọwa
| Ngwakọta PCR-1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Njikwa Dị n'ime | Njikwa Dị n'ime |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Agụụ, eriri afọ dị ọcha, swab rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Nkọwapụta | a) Ngwa ahụ na-achọpụta ntụaka ụlọ ọrụ a na-ahazi nke ọma, nsonaazụ ya na-emezukwa ihe achọrọ maka ntụaka ndị kwekọrọ. b) Nsonaazụ nke ule mmeghachi omume na-egosi na ngwa a enweghị mmeghachi omume mmegharị ahụ na nje ndị ọzọ na-akpata iku ume, dịka Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, ma ọ bụ ihe atụ nwere mkpụrụ ndụ ihe nketa ndị ọzọ na-eguzogide ọgwụ CTX, mecA, SME, SHV, TEM, wdg. c) Mgbochi mgbochi: A na-ahọrọ Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin maka nnwale nnyonye anya, nsonaazụ ya na-egosikwa na ihe ndị a kpọtụrụ aha n'elu enweghị mmeghachi omume na nchọpụta nke mkpụrụ ndụ ihe nketa ndị na-eguzogide carbapenem KPC, NDM, OXA48, OXA23, VIM, na IMP. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR nke QuantStudio®5 n'oge dị adị Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR oge-oge LightCycler®480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A,Hangzhouteknụzụ biore) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1.
Ihe na-ewepụta ihe a tụrụ aro: Ngwa DNA/RNA nke izugbe nke Macro & Micro-Test (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Tinye 200μL nke saline nkịtị na thallus precipitate. Nzọụkwụ ndị na-esote kwesịrị ịgbaso ntuziaka maka mwepụta, olu mgbapụta akwadoro bụ100μL.
Nhọrọ nke abụọ.
Ihe na-eme ka a na-ewepụta ihe a tụrụ aro: Mwepụ ma ọ bụ Mwepụ ihe na-eme ka ihe dị ọcha nke Nucleic Acid (YDP302) nke Tiangen Biotech (Beijing) Co., Ltd. A ga-amalite mwepụ ahụ dịka usoro nke abụọ nke ntuziaka maka ojiji si dị (tinye 200μL nke ihe na-eme ka ihe dị n'ime GA na thallus precipitate, ma maa jijiji ruo mgbe thallus kwụsịrị kpamkpam). Jiri mmiri na-enweghị RNase/DNase maka mwepụ, olu mgbapụta ahụ dịkwa 100μL.
Nhọrọ nke 3.
Ihe na-eme ka a na-ewepụta ihe: Ihe na-eme ka ihe nlele Macro & Micro-Test dị nro sachaa ihe nlele sputum site na ịgbakwunye 1mL nke saline nkịtị na ihe na-eme ka ihe dị n'ime thallus a gwọrọ agwọ, nke a na-etinye n'ime 13000r/min maka nkeji ise, a na-atụfu ihe na-eme ka ihe dị n'ime ya dị ntakịrị (dobe 10-20µL nke ihe na-eme ka ihe dị n'ime ya dị ọcha). Maka swab dị ọcha na nke dị n'akụkụ aka nri, tinye 50μL nke ihe na-eme ka ihe dị n'ime ya dị kpọmkwem na ihe na-eme ka ihe dị n'ime thallus a gwọrọ agwọ, a ga-ewepụtakwa usoro ndị na-esote dịka ntuziaka maka ojiji si dị.
