Mycobacterium ụkwara nta Nucleic Acid na Rifampicin (RIF) ， Isoniazid Resistance (INH)

Nkọwa dị mkpirikpi:

Ngwaahịa a dabara adaba maka nchọpụta qualitative nke Mycobacterium ụkwara nta DNA na sputum sample mmadụ in vitro, yana ngbanwe homozygous na 507-533 amino acid codon region (81bp, rifampicin resistance determining region) nke rpoB gene na-akpata Mycobacterium ụkwara nta. nguzogide rifampicin.

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HWTS-RT147 Mycobacterium ụkwara nta Nucleic Acid na Rifampicin(RIF), Ngwa nchọpụta Isoniazid Resistance (INH) (Melting Curve)

Epidemiology

Mycobacterium ụkwara nta, obere oge dị ka Tubercle bacillus (TB), bụ nje nje na-akpata ụkwara nta.Ugbu a, ọgwụ mgbochi ụkwara nta nke mbụ a na-ejikarị gụnyere isoniazid, rifampicin na ethambutol, wdg. Ọgwụ mgbochi ụkwara nta nke abụọ gụnyere fluoroquinolones, amikacin na kanamycin, wdg. Ọgwụ ọhụrụ emepụtara bụ linezolid, bedaquiline na delamani, wdg. Otú ọ dị, n'ihi iji ọgwụ mgbochi ụkwara nta na-ezighị ezi na njirimara nke mgbidi cell nke ụkwara nta nke mycobacterium, ụkwara nta mycobacterium na-emepụta ọgwụ na-eguzogide ọgwụ mgbochi ụkwara nta, nke na-eweta nnukwu nsogbu na mgbochi na ọgwụgwọ ụkwara nta.

Ọwa

Aha ebumnuche			Onye nta akụkọ	Quencher
Nchekwa mmeghachi omumeA	Nchekwa mmeghachi omumeB	Nchekwa mmeghachi omumeC	Onye nta akụkọ	Quencher
rpoB 507-514	rpoB 513-520	IS6110	FAM	Ọ dịghị
rpoB 520-527	rpoB 527-533	/	CY5	Ọ dịghị
/	/	Njikwa ime	HEX (VIC)	Ọ dịghị

Nchekwa mmeghachi omumeD	Onye nta akụkọ	Quencher
Mpaghara ndị na-akwalite InhA -15C>T, -8T>A, -8T>C	FAM	Ọ dịghị
KatG 315 codon 315G>A,315G>C	CY5	Ọ dịghị
AhpC mpaghara nkwalite -12C>T, -6G>A	ROX	Ọ dịghị

Nka nka

Nchekwa	≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	sputum
CV	≤5.0%
LoD	LoD nke mycobacterium ụkwara nta nke mba bụ 50 bacteria / ml.LoD nke ụdị anụ ọhịa na-eguzogide rifampicin bụ 2 × 10³bacteria/ml, na LoD nke ụdị mutant bụ 2 × 10³nje bacteria/ml.LoD nke ụdị anụ ọhịa isoniazid na-eguzogide nje bacteria bụ 2x10³nje bacteria/ml, na LoD nke nje bacteria mutant bụ 2x10³nje bacteria/ml.
Nkọwapụta	Nsonaazụ ule cross gosiri na ọ dịghị cross mmeghachi omume na nchọpụta nke mmadụ genome, ndị ọzọ na-abụghị ụkwara nta mycobacteria na oyi baa na ngwa ngwa;Enweghị mmeghachi omume ọ bụla achọpụtara na saịtị mmụgharị nke mkpụrụ ndụ ihe nketa ndị ọzọ na-eguzogide ọgwụ na ụkwara nta Mycobacterium nke anụ ọhịa.
Ngwa ndị ọdabara	SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), Sistemụ PCR nke BioRad CFX96, Teknụzụ Hangzhou Bioer QuantGene 9600 PCR ezigbo oge, QuantStudio^®Sistemụ PCR 5 ezigbo oge.